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Q12WX1

- Q12WX1_METBU

UniProt

Q12WX1 - Q12WX1_METBU

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Protein

CCA-adding enzyme

Gene
cca, Mbur_1130
Organism
Methanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 / ACE-M)
Status
Unreviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate By similarity.UniRule annotation

Catalytic activityi

A tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei52 – 521ATP or CTP By similarityUniRule annotation
Binding sitei55 – 551ATP or CTP By similarityUniRule annotation
Metal bindingi64 – 641Magnesium By similarityUniRule annotation
Metal bindingi66 – 661Magnesium By similarityUniRule annotation
Metal bindingi118 – 1181Magnesium By similarityUniRule annotation
Binding sitei141 – 1411ATP or CTP By similarityUniRule annotation
Binding sitei160 – 1601ATP or CTP By similarityUniRule annotation
Binding sitei169 – 1691ATP or CTP By similarityUniRule annotation

GO - Molecular functioni

  1. ATP:3'-cytidine-cytidine-tRNA adenylyltransferase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-HAMAP
  3. CTP:3'-cytidine-tRNA cytidylyltransferase activity Source: UniProtKB-EC
  4. CTP:tRNA cytidylyltransferase activity Source: UniProtKB-EC
  5. magnesium ion binding Source: UniProtKB-HAMAP
  6. tRNA adenylyltransferase activity Source: UniProtKB-HAMAP
  7. tRNA binding Source: UniProtKB-HAMAP
  8. tRNA cytidylyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. RNA repair Source: UniProtKB-KW
  2. tRNA 3'-terminal CCA addition Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

NucleotidyltransferaseUniRule annotationImported, Transferase

Keywords - Biological processi

RNA repairUniRule annotation, tRNA processingUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding, RNA-bindingUniRule annotation

Enzyme and pathway databases

BioCyciMBUR259564:GHBZ-1151-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
CCA-adding enzymeUniRule annotation (EC:2.7.7.72UniRule annotation)
Alternative name(s):
CCA tRNA nucleotidyltransferase
tRNA CCA-pyrophosphorylase
tRNA adenylyl-/cytidylyl- transferase
tRNA nucleotidyltransferase
tRNA-NT
Gene namesi
Name:ccaUniRule annotationImported
Ordered Locus Names:Mbur_1130Imported
OrganismiMethanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 / ACE-M)Imported
Taxonomic identifieri259564 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanococcoides
ProteomesiUP000001979: Chromosome

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi259564.Mbur_1130.

Structurei

3D structure databases

ProteinModelPortaliQ12WX1.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1746.
HOGENOMiHOG000226400.
KOiK07558.
OMAiDRTPFHN.

Family and domain databases

HAMAPiMF_01264. CCA_arch.
InterProiIPR008229. CCA-adding_arc.
IPR002934. Nucleotidyltransferase.
IPR011068. NuclTrfase_I_C.
IPR015329. tRNA_NucTransf2.
[Graphical view]
PfamiPF01909. NTP_transf_2. 1 hit.
PF09249. tRNA_NucTransf2. 1 hit.
[Graphical view]
PIRSFiPIRSF005335. CCA_arch. 1 hit.
SUPFAMiSSF55003. SSF55003. 1 hit.
TIGRFAMsiTIGR03671. cca_archaeal. 1 hit.

Sequencei

Sequence statusi: Complete.

Q12WX1-1 [UniParc]FASTAAdd to Basket

« Hide

MESLKKSILE KIKPTEKERE HLTKVADELM YKIDCLTFKV GLVGVKTQLV    50
GSAARGTWIS GTHDLDIFIT FPDDTSREDL ESYGLSVGRQ VAKEAEHWDE 100
HYAEHPYVKM RYKGFDVDLV PCYSVSSASS IISAVDRTPF HNEFIKANLN 150
GLEDEVLLLK QFMRGTGVYG SELKTEGFSG YLTELLVINY GSFEKVLEAA 200
SNWRPGLLLD ILEHGKLKFE DPLVVIDPTD PKRNVAAALS LDKFCTFVDM 250
ARSFLCNPDE SMFFSSLIPP ISDNEIVERL EKKGSSLLAI VFKTPDVVDD 300
IFYPQFAKME NSVVPMLDKN EFTVLKAGKW SGEDSVVMLE LMSGTLPNVK 350
MHRGPPVWVR DHAEKFRDKY VDNEDAYSFT IKDGFYVAEI PRKHISAVEL 400
LKKELANCSL GKHISKSVKE GFEILEGEQV LELKEDGFRS YLKSWL 446
Length:446
Mass (Da):50,467
Last modified:August 22, 2006 - v1
Checksum:i5B4AD00060D50EEB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000300 Genomic DNA. Translation: ABE52055.1.
RefSeqiYP_565805.1. NC_007955.1.

Genome annotation databases

EnsemblBacteriaiABE52055; ABE52055; Mbur_1130.
GeneIDi3997737.
KEGGimbu:Mbur_1130.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000300 Genomic DNA. Translation: ABE52055.1 .
RefSeqi YP_565805.1. NC_007955.1.

3D structure databases

ProteinModelPortali Q12WX1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 259564.Mbur_1130.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABE52055 ; ABE52055 ; Mbur_1130 .
GeneIDi 3997737.
KEGGi mbu:Mbur_1130.

Phylogenomic databases

eggNOGi COG1746.
HOGENOMi HOG000226400.
KOi K07558.
OMAi DRTPFHN.

Enzyme and pathway databases

BioCyci MBUR259564:GHBZ-1151-MONOMER.

Family and domain databases

HAMAPi MF_01264. CCA_arch.
InterProi IPR008229. CCA-adding_arc.
IPR002934. Nucleotidyltransferase.
IPR011068. NuclTrfase_I_C.
IPR015329. tRNA_NucTransf2.
[Graphical view ]
Pfami PF01909. NTP_transf_2. 1 hit.
PF09249. tRNA_NucTransf2. 1 hit.
[Graphical view ]
PIRSFi PIRSF005335. CCA_arch. 1 hit.
SUPFAMi SSF55003. SSF55003. 1 hit.
TIGRFAMsi TIGR03671. cca_archaeal. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 6242 / NBRC 107633 / OCM 468 / ACE-M.

Entry informationi

Entry nameiQ12WX1_METBU
AccessioniPrimary (citable) accession number: Q12WX1
Entry historyi
Integrated into UniProtKB/TrEMBL: August 22, 2006
Last sequence update: August 22, 2006
Last modified: June 11, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Miscellaneous

A single active site specifically recognizes both ATP and CTP and is responsible for their addition By similarity.UniRule annotation

Keywords - Technical termi

Complete proteome

External Data

Dasty 3

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