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Q12WX1

- Q12WX1_METBU

UniProt

Q12WX1 - Q12WX1_METBU

Protein

CCA-adding enzyme

Gene

cca

Organism
Methanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 / ACE-M)
Status
Unreviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 51 (01 Oct 2014)
      Sequence version 1 (22 Aug 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate.UniRule annotation

    Catalytic activityi

    A tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei52 – 521ATP or CTPUniRule annotation
    Binding sitei55 – 551ATP or CTPUniRule annotation
    Metal bindingi64 – 641MagnesiumUniRule annotation
    Metal bindingi66 – 661MagnesiumUniRule annotation
    Metal bindingi118 – 1181MagnesiumUniRule annotation
    Binding sitei141 – 1411ATP or CTPUniRule annotation
    Binding sitei160 – 1601ATP or CTPUniRule annotation
    Binding sitei169 – 1691ATP or CTPUniRule annotation

    GO - Molecular functioni

    1. ATP:3'-cytidine-cytidine-tRNA adenylyltransferase activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-HAMAP
    3. CTP:3'-cytidine-tRNA cytidylyltransferase activity Source: UniProtKB-EC
    4. CTP:tRNA cytidylyltransferase activity Source: UniProtKB-EC
    5. magnesium ion binding Source: UniProtKB-HAMAP
    6. tRNA adenylyltransferase activity Source: UniProtKB-HAMAP
    7. tRNA binding Source: UniProtKB-HAMAP
    8. tRNA cytidylyltransferase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. RNA repair Source: UniProtKB-KW
    2. tRNA 3'-terminal CCA addition Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    NucleotidyltransferaseUniRule annotationImported, Transferase

    Keywords - Biological processi

    RNA repairUniRule annotation, tRNA processingUniRule annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding, RNA-bindingUniRule annotation

    Enzyme and pathway databases

    BioCyciMBUR259564:GHBZ-1151-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    CCA-adding enzymeUniRule annotation (EC:2.7.7.72UniRule annotation)
    Alternative name(s):
    CCA tRNA nucleotidyltransferaseUniRule annotation
    tRNA CCA-pyrophosphorylaseUniRule annotation
    tRNA adenylyl-/cytidylyl- transferaseUniRule annotation
    tRNA nucleotidyltransferaseUniRule annotation
    tRNA-NTUniRule annotation
    Gene namesi
    Name:ccaUniRule annotationImported
    Ordered Locus Names:Mbur_1130Imported
    OrganismiMethanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 / ACE-M)Imported
    Taxonomic identifieri259564 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanococcoides
    ProteomesiUP000001979: Chromosome

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi259564.Mbur_1130.

    Structurei

    3D structure databases

    ProteinModelPortaliQ12WX1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Archaeal CCA-adding enzyme subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1746.
    HOGENOMiHOG000226400.
    KOiK07558.
    OMAiDRTPFHN.

    Family and domain databases

    HAMAPiMF_01264. CCA_arch.
    InterProiIPR008229. CCA-adding_arc.
    IPR002934. Nucleotidyltransferase.
    IPR011068. NuclTrfase_I_C.
    IPR015329. tRNA_NucTransf2.
    [Graphical view]
    PfamiPF01909. NTP_transf_2. 1 hit.
    PF09249. tRNA_NucTransf2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005335. CCA_arch. 1 hit.
    SUPFAMiSSF55003. SSF55003. 1 hit.
    TIGRFAMsiTIGR03671. cca_archaeal. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q12WX1-1 [UniParc]FASTAAdd to Basket

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    MESLKKSILE KIKPTEKERE HLTKVADELM YKIDCLTFKV GLVGVKTQLV    50
    GSAARGTWIS GTHDLDIFIT FPDDTSREDL ESYGLSVGRQ VAKEAEHWDE 100
    HYAEHPYVKM RYKGFDVDLV PCYSVSSASS IISAVDRTPF HNEFIKANLN 150
    GLEDEVLLLK QFMRGTGVYG SELKTEGFSG YLTELLVINY GSFEKVLEAA 200
    SNWRPGLLLD ILEHGKLKFE DPLVVIDPTD PKRNVAAALS LDKFCTFVDM 250
    ARSFLCNPDE SMFFSSLIPP ISDNEIVERL EKKGSSLLAI VFKTPDVVDD 300
    IFYPQFAKME NSVVPMLDKN EFTVLKAGKW SGEDSVVMLE LMSGTLPNVK 350
    MHRGPPVWVR DHAEKFRDKY VDNEDAYSFT IKDGFYVAEI PRKHISAVEL 400
    LKKELANCSL GKHISKSVKE GFEILEGEQV LELKEDGFRS YLKSWL 446
    Length:446
    Mass (Da):50,467
    Last modified:August 22, 2006 - v1
    Checksum:i5B4AD00060D50EEB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000300 Genomic DNA. Translation: ABE52055.1.
    RefSeqiYP_565805.1. NC_007955.1.

    Genome annotation databases

    EnsemblBacteriaiABE52055; ABE52055; Mbur_1130.
    GeneIDi3997737.
    KEGGimbu:Mbur_1130.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000300 Genomic DNA. Translation: ABE52055.1 .
    RefSeqi YP_565805.1. NC_007955.1.

    3D structure databases

    ProteinModelPortali Q12WX1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 259564.Mbur_1130.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABE52055 ; ABE52055 ; Mbur_1130 .
    GeneIDi 3997737.
    KEGGi mbu:Mbur_1130.

    Phylogenomic databases

    eggNOGi COG1746.
    HOGENOMi HOG000226400.
    KOi K07558.
    OMAi DRTPFHN.

    Enzyme and pathway databases

    BioCyci MBUR259564:GHBZ-1151-MONOMER.

    Family and domain databases

    HAMAPi MF_01264. CCA_arch.
    InterProi IPR008229. CCA-adding_arc.
    IPR002934. Nucleotidyltransferase.
    IPR011068. NuclTrfase_I_C.
    IPR015329. tRNA_NucTransf2.
    [Graphical view ]
    Pfami PF01909. NTP_transf_2. 1 hit.
    PF09249. tRNA_NucTransf2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005335. CCA_arch. 1 hit.
    SUPFAMi SSF55003. SSF55003. 1 hit.
    TIGRFAMsi TIGR03671. cca_archaeal. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 6242 / NBRC 107633 / OCM 468 / ACE-MImported.

    Entry informationi

    Entry nameiQ12WX1_METBU
    AccessioniPrimary (citable) accession number: Q12WX1
    Entry historyi
    Integrated into UniProtKB/TrEMBL: August 22, 2006
    Last sequence update: August 22, 2006
    Last modified: October 1, 2014
    This is version 51 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Miscellaneous

    A single active site specifically recognizes both ATP and CTP and is responsible for their addition.UniRule annotation

    Keywords - Technical termi

    Complete proteome, Reference proteomeImported

    External Data

    Dasty 3