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Protein

CCA-adding enzyme

Gene

cca

Organism
Methanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 / ACE-M)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate.UniRule annotation

Miscellaneous

A single active site specifically recognizes both ATP and CTP and is responsible for their addition.UniRule annotation

Catalytic activityi

A tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei52ATP or CTPUniRule annotation1
Binding sitei55ATP or CTPUniRule annotation1
Metal bindingi64MagnesiumUniRule annotation1
Metal bindingi66MagnesiumUniRule annotation1
Metal bindingi118MagnesiumUniRule annotation1
Binding sitei141ATP or CTPUniRule annotation1
Binding sitei160ATP or CTPUniRule annotation1
Binding sitei169ATP or CTPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferaseUniRule annotationImported, RNA-bindingUniRule annotation, Transferase
Biological processRNA repairUniRule annotation, tRNA processingUniRule annotation
LigandATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
CCA-adding enzymeUniRule annotation (EC:2.7.7.72UniRule annotation)
Alternative name(s):
CCA tRNA nucleotidyltransferaseUniRule annotation
tRNA CCA-pyrophosphorylaseUniRule annotation
tRNA adenylyl-/cytidylyl- transferaseUniRule annotation
tRNA nucleotidyltransferaseUniRule annotation
tRNA-NTUniRule annotation
Gene namesi
Name:ccaUniRule annotationImported
Ordered Locus Names:Mbur_1130Imported
OrganismiMethanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 / ACE-M)Imported
Taxonomic identifieri259564 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanococcoides
Proteomesi
  • UP000001979 Componenti: Chromosome

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi259564.Mbur_1130.

Structurei

3D structure databases

ProteinModelPortaliQ12WX1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini39 – 140NTP_transf_2InterPro annotationAdd BLAST102
Domaini154 – 264tRNA_NucTransf2InterPro annotationAdd BLAST111

Sequence similaritiesi

Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Archaeal CCA-adding enzyme subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04249. Archaea.
COG1746. LUCA.
HOGENOMiHOG000226400.
KOiK07558.
OMAiVDIVPCY.
OrthoDBiPOG093Z01VB.

Family and domain databases

HAMAPiMF_01264. CCA_arch. 1 hit.
InterProiView protein in InterPro
IPR008229. CCA-adding_arc.
IPR011068. NuclTrfase_I_C.
IPR002934. Polymerase_NTP_transf_dom.
IPR015329. tRNA_NucTransf2.
PfamiView protein in Pfam
PF01909. NTP_transf_2. 1 hit.
PF09249. tRNA_NucTransf2. 1 hit.
PIRSFiPIRSF005335. CCA_arch. 1 hit.
SUPFAMiSSF55003. SSF55003. 1 hit.
TIGRFAMsiTIGR03671. cca_archaeal. 1 hit.

Sequencei

Sequence statusi: Complete.

Q12WX1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESLKKSILE KIKPTEKERE HLTKVADELM YKIDCLTFKV GLVGVKTQLV
60 70 80 90 100
GSAARGTWIS GTHDLDIFIT FPDDTSREDL ESYGLSVGRQ VAKEAEHWDE
110 120 130 140 150
HYAEHPYVKM RYKGFDVDLV PCYSVSSASS IISAVDRTPF HNEFIKANLN
160 170 180 190 200
GLEDEVLLLK QFMRGTGVYG SELKTEGFSG YLTELLVINY GSFEKVLEAA
210 220 230 240 250
SNWRPGLLLD ILEHGKLKFE DPLVVIDPTD PKRNVAAALS LDKFCTFVDM
260 270 280 290 300
ARSFLCNPDE SMFFSSLIPP ISDNEIVERL EKKGSSLLAI VFKTPDVVDD
310 320 330 340 350
IFYPQFAKME NSVVPMLDKN EFTVLKAGKW SGEDSVVMLE LMSGTLPNVK
360 370 380 390 400
MHRGPPVWVR DHAEKFRDKY VDNEDAYSFT IKDGFYVAEI PRKHISAVEL
410 420 430 440
LKKELANCSL GKHISKSVKE GFEILEGEQV LELKEDGFRS YLKSWL
Length:446
Mass (Da):50,467
Last modified:August 22, 2006 - v1
Checksum:i5B4AD00060D50EEB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000300 Genomic DNA. Translation: ABE52055.1.
RefSeqiWP_011499202.1. NC_007955.1.

Genome annotation databases

EnsemblBacteriaiABE52055; ABE52055; Mbur_1130.
GeneIDi3997737.
KEGGimbu:Mbur_1130.

Similar proteinsi

Entry informationi

Entry nameiQ12WX1_METBU
AccessioniPrimary (citable) accession number: Q12WX1
Entry historyiIntegrated into UniProtKB/TrEMBL: August 22, 2006
Last sequence update: August 22, 2006
Last modified: September 27, 2017
This is version 68 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported