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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Methanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 / ACE-M)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei634 – 6341ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMBUR259564:GHBZ-1382-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Mbur_1356
OrganismiMethanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 / ACE-M)
Taxonomic identifieri259564 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanococcoides
ProteomesiUP000001979 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 962962Leucine--tRNA ligasePRO_1000009371Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi259564.Mbur_1356.

Structurei

3D structure databases

ProteinModelPortaliQ12WA2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi41 – 5111"HIGH" regionAdd
BLAST
Motifi631 – 6355"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000216620.
KOiK01869.
OMAiNCFVEHE.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_A. Leu_tRNA_synth_A.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00395. leuS_arch. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12WA2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQQDYNSSNI EQKWQQKWNE SKVFEAEADD RDKYFITIPY PYLNGNLHAG
60 70 80 90 100
HTRTFTIGDV VARYKRMMGN NVLYPMGFHV TGTPIVGLAE LIQNRDPETM
110 120 130 140 150
KVYTEFHGIP VETLKGMDTP EKIVDYFSVE AERSMRSIGY SIDWRRKFTT
160 170 180 190 200
TDPNYKKFIE WQFNLLYEKD LIVKGSHPVK WCPNDDNPVE DHDILHGEEA
210 220 230 240 250
TIIDYTLVKF KYDGMIIPCA TLRPETVFGV TNLWINPDLE HVKIKVTFEG
260 270 280 290 300
REEVWVVSKE AYRKLIFTDR EVEFIEDVDA SSLIGIKVTN PLNDAQVITL
310 320 330 340 350
PASFVKGENG SGIVMSVPSH APYDYLALRD LYDKDLREYG ITEDLRELKF
360 370 380 390 400
ISLIKVKEFG EFPAIEAVEQ FGVKDQDDPK AEEATKIVYR REFHGGVLKE
410 420 430 440 450
NTGKYSGMAV SKIKDVLTRD LIEMGIGEVF YEFSEPVVCR CGTPCVVNMV
460 470 480 490 500
KGQWFLNYSN PEWKDKVYRC IENMDIIPED LRVEFNNKVD WLKDKACARK
510 520 530 540 550
KGLGTLLPFD NQWLIESLGD STIYMSYYII AKFIAMGIET EQLVPELFDH
560 570 580 590 600
VLLKKCSLET AAERSGIDAN IIEQISSDFE YWYPVDLRSS GKDLIPNHLL
610 620 630 640 650
FFLFHHVAIF DEDKWPRAIA INGFVSLEGK KMSKSKGPLL TLNDAITNYG
660 670 680 690 700
ADISRMYILS SAEQMQDADW KNSGIETARK QIERFYNFSK DIIGSGIPTC
710 720 730 740 750
NVENLKGIDK WMLSRLQQRI LETNEALDTI RTRNALQNAY FLLFNDIRWY
760 770 780 790 800
QKRGGNALLC EVLDVWIRLM APFTPHICEE IWEAIGHTDN DLISLADYPQ
810 820 830 840 850
YDESLVDTQA EFTEELIGGT LSDVDEIIRV TKLTPKKAIL YTSPEWKMET
860 870 880 890 900
FKKALSMQKE GNLNPGILIK DLMRDPEMRS HGKEVPKFAQ KVVSDITAMN
910 920 930 940 950
EEKFDTLSNF DLDEKIALEE NLEFFKNELG CSVEIYSADN AEYDPENKAR
960
FAYPLRPAIY LE
Length:962
Mass (Da):110,794
Last modified:August 22, 2006 - v1
Checksum:i735ABD6E1D31D010
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000300 Genomic DNA. Translation: ABE52274.1.
RefSeqiWP_011499419.1. NC_007955.1.
YP_566024.1. NC_007955.1.

Genome annotation databases

EnsemblBacteriaiABE52274; ABE52274; Mbur_1356.
GeneIDi3997572.
KEGGimbu:Mbur_1356.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000300 Genomic DNA. Translation: ABE52274.1.
RefSeqiWP_011499419.1. NC_007955.1.
YP_566024.1. NC_007955.1.

3D structure databases

ProteinModelPortaliQ12WA2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi259564.Mbur_1356.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABE52274; ABE52274; Mbur_1356.
GeneIDi3997572.
KEGGimbu:Mbur_1356.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000216620.
KOiK01869.
OMAiNCFVEHE.

Enzyme and pathway databases

BioCyciMBUR259564:GHBZ-1382-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_A. Leu_tRNA_synth_A.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00395. leuS_arch. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 6242 / NBRC 107633 / OCM 468 / ACE-M.

Entry informationi

Entry nameiSYL_METBU
AccessioniPrimary (citable) accession number: Q12WA2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 22, 2006
Last modified: March 4, 2015
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.