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Q12WA2 (SYL_METBU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Mbur_1356
OrganismMethanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 / ACE-M) [Complete proteome] [HAMAP]
Taxonomic identifier259564 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanococcoides

Protein attributes

Sequence length962 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 962962Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009371

Regions

Motif41 – 5111"HIGH" region HAMAP-Rule MF_00049
Motif631 – 6355"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6341ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q12WA2 [UniParc].

Last modified August 22, 2006. Version 1.
Checksum: 735ABD6E1D31D010

FASTA962110,794
        10         20         30         40         50         60 
MQQDYNSSNI EQKWQQKWNE SKVFEAEADD RDKYFITIPY PYLNGNLHAG HTRTFTIGDV 

        70         80         90        100        110        120 
VARYKRMMGN NVLYPMGFHV TGTPIVGLAE LIQNRDPETM KVYTEFHGIP VETLKGMDTP 

       130        140        150        160        170        180 
EKIVDYFSVE AERSMRSIGY SIDWRRKFTT TDPNYKKFIE WQFNLLYEKD LIVKGSHPVK 

       190        200        210        220        230        240 
WCPNDDNPVE DHDILHGEEA TIIDYTLVKF KYDGMIIPCA TLRPETVFGV TNLWINPDLE 

       250        260        270        280        290        300 
HVKIKVTFEG REEVWVVSKE AYRKLIFTDR EVEFIEDVDA SSLIGIKVTN PLNDAQVITL 

       310        320        330        340        350        360 
PASFVKGENG SGIVMSVPSH APYDYLALRD LYDKDLREYG ITEDLRELKF ISLIKVKEFG 

       370        380        390        400        410        420 
EFPAIEAVEQ FGVKDQDDPK AEEATKIVYR REFHGGVLKE NTGKYSGMAV SKIKDVLTRD 

       430        440        450        460        470        480 
LIEMGIGEVF YEFSEPVVCR CGTPCVVNMV KGQWFLNYSN PEWKDKVYRC IENMDIIPED 

       490        500        510        520        530        540 
LRVEFNNKVD WLKDKACARK KGLGTLLPFD NQWLIESLGD STIYMSYYII AKFIAMGIET 

       550        560        570        580        590        600 
EQLVPELFDH VLLKKCSLET AAERSGIDAN IIEQISSDFE YWYPVDLRSS GKDLIPNHLL 

       610        620        630        640        650        660 
FFLFHHVAIF DEDKWPRAIA INGFVSLEGK KMSKSKGPLL TLNDAITNYG ADISRMYILS 

       670        680        690        700        710        720 
SAEQMQDADW KNSGIETARK QIERFYNFSK DIIGSGIPTC NVENLKGIDK WMLSRLQQRI 

       730        740        750        760        770        780 
LETNEALDTI RTRNALQNAY FLLFNDIRWY QKRGGNALLC EVLDVWIRLM APFTPHICEE 

       790        800        810        820        830        840 
IWEAIGHTDN DLISLADYPQ YDESLVDTQA EFTEELIGGT LSDVDEIIRV TKLTPKKAIL 

       850        860        870        880        890        900 
YTSPEWKMET FKKALSMQKE GNLNPGILIK DLMRDPEMRS HGKEVPKFAQ KVVSDITAMN 

       910        920        930        940        950        960 
EEKFDTLSNF DLDEKIALEE NLEFFKNELG CSVEIYSADN AEYDPENKAR FAYPLRPAIY 


LE 

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References

[1]"Complete sequence of Methanococcoides burtonii DSM 6242."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Kadner K., Aerts A., Dehal P., Pitluck S., Martinez M., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Anderson I., Franzmann P., Thomas T., Saunders N., Cavicchioli R., Sowers K., Richardson P.
Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 6242 / NBRC 107633 / OCM 468 / ACE-M.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000300 Genomic DNA. Translation: ABE52274.1.
RefSeqYP_566024.1. NC_007955.1.

3D structure databases

ProteinModelPortalQ12WA2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING259564.Mbur_1356.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABE52274; ABE52274; Mbur_1356.
GeneID3997572.
KEGGmbu:Mbur_1356.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000216620.
KOK01869.
OMAYSKFVEW.

Enzyme and pathway databases

BioCycMBUR259564:GHBZ-1382-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_A. Leu_tRNA_synth_A.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
[Graphical view]
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00395. leuS_arch. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_METBU
AccessionPrimary (citable) accession number: Q12WA2
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 22, 2006
Last modified: May 14, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries