Reviewed, UniProtKB/Swiss-Prot Q12VU4 (MTRE_METBU)

Names and origin

Protein names

Recommended name:
    Tetrahydromethanopterin S-methyltransferase subunit E
    EC=2.1.1.86
Alternative name(s):
    N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit E

Gene names

Name:	mtrE
Ordered Locus Names:	Mbur_1525

Organism

Methanococcoides burtonii (strain DSM 6242) [Complete proteome] [HAMAP]

Taxonomic identifier

259564 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanococcoides

Protein attributes

Sequence length	301 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step By similarity.
Catalytic activity	5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate = 5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate. HAMAP MF_01098
Subunit structure	The complex is composed of 8 subunits; mtrA, mtrB, mtrC, mtrD, mtrE, mtrF, mtrG and mtrH By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the mtrE family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	one-carbon compound metabolic process Inferred from electronic annotation. Source: HAMAP sodium ion transport Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell vesicle membrane Inferred from electronic annotation. Source: InterPro
Molecular function	tetrahydromethanopterin S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 301	301	Tetrahydromethanopterin S-methyltransferase subunit E HAMAP MF_01098		PRO_1000064943
Regions
	Transmembrane	85 – 105	21	Potential
	Transmembrane	130 – 150	21	Potential
	Transmembrane	151 – 171	21	Potential
	Transmembrane	232 – 252	21	Potential
	Transmembrane	258 – 278	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q12VU4-1 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: E88BD4537D22C5C5	FASTA	301	31,934
	10 20 30 40 50 60 MEPLMGMGVL ALMGAAATIA GTTEDLESDV GSQSNPNSQV QLAPQMMYPH RIYNKAISGE 70 80 90 100 110 120 PPSNALICAI GGTVASVLMT ANLSVIFAIA IGALVASAVH GTYCITAYMG RTASQKRFRQ 130 140 150 160 170 180 PIYLDILRSH TPVMMGYAFI TTFCILVVSY IMVAVLAHPF PLTLLAFIWG ITVGAIGSST 190 200 210 220 230 240 GDVHYGAERE FQNVEFGSGL NAANSGNIVR KAESGLRNGI DNSWFCAKFG GPVTGLAFGM 250 260 270 280 290 300 TVFLSGWVTA VFNPAISLTM GWLSVAAGVI LVLLLIIWNR KIEVAARKAF GPYKEEEEVA A

References

[1]

"Complete sequence of Methanococcoides burtonii DSM 6242." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Kadner K., Aerts A., Dehal P., Pitluck S., Martinez M., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Anderson I., Franzmann P., Thomas T., Saunders N., Cavicchioli R., Sowers K., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000300 Genomic DNA. Translation: ABE52432.1.
RefSeq	YP_566182.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3997348.
GenomeReviews	Gene locus Mbur_1525 in contig CP000300_GR.
KEGG	mbu:Mbur_1525.
NMPDR	fig|259564.8.peg.1452.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q12VU4.
OMA	Q12VU4. SNPNSQV.
Enzyme and pathway databases
BioCyc	MBUR259564:MBUR_1525-MON.
Family and domain databases
HAMAP	MF_01098. [Tree]
InterPro	IPR005780. MeTrfase_E. [Graphical view]
Pfam	PF04206. MtrE. 1 hit. [Graphical view]
PIRSF	PIRSF016509. MtrE. 1 hit.
TIGRFAMs	TIGR01113. mtrE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MTRE_METBU

Accession

Primary (citable) accession number: Q12VU4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	August 22, 2006
Last modified:	June 16, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)