Reviewed, UniProtKB/Swiss-Prot Q12VF1 (ASPD_METBU)

Names and origin

Protein names

Recommended name:
    Probable L-aspartate dehydrogenase
    EC=1.4.1.21

Gene names

Name:	nadX
Ordered Locus Names:	Mbur_1681

Organism

Methanococcoides burtonii (strain DSM 6242) [Complete proteome] [HAMAP]

Taxonomic identifier

259564 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanococcoides

Protein attributes

Sequence length	271 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically catalyzes the NAD or NADP-dependent dehydrogenation of L-aspartate to iminoaspartate By similarity.
Catalytic activity	L-aspartate + H2O + NAD(P)+ = oxaloacetate + NH3 + NAD(P)H. HAMAP MF_01265
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (dehydrogenase route): step 1/1. HAMAP MF_01265
Miscellaneous	The iminoaspartate product is unstable in aqueous solution and can decompose to oxaloacetate and ammonia By similarity.
Sequence similarities	Belongs to the L-aspartate dehydrogenase family.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP NADP catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: HAMAP NADP or NADPH binding Inferred from electronic annotation. Source: HAMAP aspartate dehydrogenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 271	271	Probable L-aspartate dehydrogenase HAMAP MF_01265		PRO_1000067304
Sites
	Active site	222	1	By similarity
	Binding site	124	1	NAD; via amide nitrogen By similarity
	Binding site	192	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q12VF1-1 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 00D97CE0518CAB6A	FASTA	271	29,094
	10 20 30 40 50 60 MLKIGVFGCG AIGTELCKAI DSGHIEVELY AVYDRHEQSI INLKEQLKNT DPKVLEIVEM 70 80 90 100 110 120 VKHVDLVVEC ASQQAVYDVV PTTLHAKCDV MVISVGAFAD KKLLDTTFDI AKEYGCKIYF 130 140 150 160 170 180 PSGAIVGLDG LKSASAASIY SVTLTTQKHP RSFEGAPYIV QNNIDLDSIK GKTVLFEGMA 190 200 210 220 230 240 SEAVKAFPSN VNVAASLSIA GIGFDKTKVK IIANPALTRN IHEITVEGEF GMFTTRVENV 250 260 270 PAPSNPKTSY LAALSAISTL KKIADPLQVG T

References

[1]

"Complete sequence of Methanococcoides burtonii DSM 6242." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Kadner K., Aerts A., Dehal P., Pitluck S., Martinez M., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Anderson I., Franzmann P., Thomas T., Saunders N., Cavicchioli R., Sowers K., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000300 Genomic DNA. Translation: ABE52575.1.
RefSeq	YP_566325.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3998077.
GenomeReviews	Gene locus Mbur_1681 in contig CP000300_GR.
KEGG	mbu:Mbur_1681.
NMPDR	fig|259564.8.peg.1603.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q12VF1.
OMA	Q12VF1. ECAGHSA.
Enzyme and pathway databases
BioCyc	MBUR259564:MBUR_1681-MON.
Family and domain databases
HAMAP	MF_01265. [Tree]
InterPro	IPR005106. Asp/hSer_DH_NAD-bd. IPR002811. Asp_DH. IPR011182. Asp_DH_NAD_syn. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01958. DUF108. 1 hit. PF03447. NAD_binding_3. 1 hit. [Graphical view]
PIRSF	PIRSF005227. Asp_dh_NAD_syn. 1 hit.
ProDom	PD017325. Asp_dh. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

ASPD_METBU

Accession

Primary (citable) accession number: Q12VF1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	August 22, 2006
Last modified:	June 16, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)