Reviewed, UniProtKB/Swiss-Prot Q12VB0 (RIFK_METBU)

Names and origin

Protein names

Recommended name:
    Riboflavin kinase
      Short name=RFK
    EC=2.7.1.161
Alternative name(s):
    CTP:riboflavin 5'-phosphotransferase
    CTP-dependent riboflavin kinase
    Flavokinase

Gene names

Name:	rfk
Ordered Locus Names:	Mbur_1724

Organism

Methanococcoides burtonii (strain DSM 6242) [Complete proteome] [HAMAP]

Taxonomic identifier

259564 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanococcoides

Protein attributes

Sequence length	223 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) By similarity.
Catalytic activity	CTP + riboflavin = CDP + FMN. HAMAP MF_01285
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1. HAMAP MF_01285
Sequence similarities	Belongs to the archaeal riboflavin kinase family.

Ontologies

Keywords
Ligand	FMN Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	FMN biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: HAMAP riboflavin kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 223	223	Riboflavin kinase HAMAP MF_01285		PRO_0000322090
Regions
	Nucleotide binding	99 – 104	6	CDP By similarity
	Nucleotide binding	198 – 201	4	CDP By similarity
	Region	1 – 89	89	Unknown HAMAP MF_01285
	Region	90 – 223	134	Riboflavin kinase HAMAP MF_01285
Sites
	Metal binding	128	1	Magnesium By similarity
	Metal binding	130	1	Magnesium By similarity
	Binding site	185	1	FMN By similarity
	Binding site	193	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q12VB0-1 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: C0CA86FEC418759A	FASTA	223	25,021
	10 20 30 40 50 60 MHRINALKHL ALLGALKKPV KISSSEFTRY TSTGSKTAAR ILKQLEEEGS IDRLIIPEGQ 70 80 90 100 110 120 MISITEKGHK WLESEFSDYK HIFCGDEDKV ELYGNVITGL GEGQYYIAQD GYGSQFEEKL 130 140 150 160 170 180 GFKPYPGTLN VRLTSHSADI LKRKSQKNII PISGFTDGQR TFGGCNCYFV EVEGVRGAVV 190 200 210 220 TPERSHYPHD LLEIISPVHL RKTLELNDGD EVKIMIEDRS ACE

References

[1]

"Complete sequence of Methanococcoides burtonii DSM 6242." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Kadner K., Aerts A., Dehal P., Pitluck S., Martinez M., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Anderson I., Franzmann P., Thomas T., Saunders N., Cavicchioli R., Sowers K., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000300 Genomic DNA. Translation: ABE52616.1.
RefSeq	YP_566366.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q12VB0.
Genome annotation databases
GeneID	3997399.
GenomeReviews	Gene locus Mbur_1724 in contig CP000300_GR.
KEGG	mbu:Mbur_1724.
NMPDR	fig|259564.8.peg.1643.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q12VB0.
OMA	GAYYVRQ.
Enzyme and pathway databases
BioCyc	MBUR259564:MBUR_1724-MON.
Family and domain databases
HAMAP	MF_01285. Fused. [Tree]
InterPro	IPR002834. Riboflavin_kinase_arc. [Graphical view]
Pfam	PF01982. CTP-dep_RFKase. 1 hit. [Graphical view]
ProDom	PD015839. DUF120. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

RIFK_METBU

Accession

Primary (citable) accession number: Q12VB0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	August 22, 2006
Last modified:	November 3, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)