Reviewed, UniProtKB/Swiss-Prot Q12V70 (RTCA_METBU)

Names and origin

Protein names

Recommended name:
    Probable RNA 3'-terminal phosphate cyclase
      Short name=RNA-3'-phosphate cyclase
      Short name=RNA cyclase
    EC=6.5.1.4

Gene names

Name:	rtcA
Ordered Locus Names:	Mbur_1766

Organism

Methanococcoides burtonii (strain DSM 6242) [Complete proteome] [HAMAP]

Taxonomic identifier

259564 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanococcoides

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) the enzyme acts on RNA-N3'P to produce RNA-N3'PP5'A; (C) a non catalytic nucleophilic attack by the adjacent 2'hydroxyl on the phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity.
Catalytic activity	ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate. HAMAP MF_00200
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-3'-phosphate cyclase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 331	331	Probable RNA 3'-terminal phosphate cyclase HAMAP MF_00200		PRO_1000012112
Sites
	Active site	301	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q12V70-1 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 24FA7EED5854BA25	FASTA	331	35,548
	10 20 30 40 50 60 MIDIDGSYGE GGGQIVRNAI ALSAVTGKAT SIKNIRKDRP NPGLSAQHVK AIGIAALLCD 70 80 90 100 110 120 AKVEGIKIGS TNIAFFPQEI RGGKYTIDIG TAGSIALLLQ CIMPIATYSN TNIKLEIKGG 130 140 150 160 170 180 TDVSWAPSID YLKNVTLSAL SKMGYRCNID ILKRGYYPRG GGIVNAIIEP SHLVPDRFSE 190 200 210 220 230 240 ERGTIRGISH CSNLPEHVAQ RQADKAKAIL ENAGHECSIE TCRTDFTSTG SGITLYCGMK 250 260 270 280 290 300 GSFVPGKRGT TAEKVGNDAA TSLLDELLTP SSVDIHLADQ LIPYLGLAEG GSFTVKEISP 310 320 330 HTKTNIWVTE KFLDVKFKIE KRNDIVKISI Q

References

[1]

"Complete sequence of Methanococcoides burtonii DSM 6242." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Kadner K., Aerts A., Dehal P., Pitluck S., Martinez M., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Anderson I., Franzmann P., Thomas T., Saunders N., Cavicchioli R., Sowers K., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000300 Genomic DNA. Translation: ABE52656.1.
RefSeq	YP_566406.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q12V70.
Genome annotation databases
GeneID	3997326.
GenomeReviews	Gene locus Mbur_1766 in contig CP000300_GR.
KEGG	mbu:Mbur_1766.
NMPDR	fig|259564.8.peg.1688.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q12V70.
OMA	EIDGSYG.
Enzyme and pathway databases
BioCyc	MBUR259564:MBUR_1766-MON.
Family and domain databases
HAMAP	MF_00200. [Tree]
InterPro	IPR000228. RNA3'_term_phos_cycl-like. IPR013796. RNA3'_term_phos_cycl_insert. IPR017770. RNA3'_term_phos_cycl_sub. [Graphical view]
Gene3D	G3DSA:3.65.10.20. RNA3'_term_phos_cycl. 1 hit.
PANTHER	PTHR11096. RNA3'_term_phos_cycl. 1 hit.
Pfam	PF01137. RTC. 1 hit. PF05189. RTC_insert. 1 hit. [Graphical view]
TIGRFAMs	TIGR03399. RNA_3prim_cycl. 1 hit.
PROSITE	PS01287. RTC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RTCA_METBU

Accession

Primary (citable) accession number: Q12V70

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 22, 2006
Last modified:	November 3, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)