ID   NADE_METBU              Reviewed;         263 AA.
AC   Q12V31;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   22-AUG-2006, sequence version 1.
DT   16-JUN-2009, entry version 21.
DE   RecName: Full=NH(3)-dependent NAD(+) synthetase;
DE            EC=6.3.1.5;
GN   Name=nadE; OrderedLocusNames=Mbur_1809;
OS   Methanococcoides burtonii (strain DSM 6242).
OC   Archaea; Euryarchaeota; Methanomicrobia; Methanosarcinales;
OC   Methanosarcinaceae; Methanococcoides.
OX   NCBI_TaxID=259564;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H.,
RA   Kadner K., Aerts A., Dehal P., Pitluck S., Martinez M., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Anderson I.,
RA   Franzmann P., Thomas T., Saunders N., Cavicchioli R., Sowers K.,
RA   Richardson P.;
RT   "Complete sequence of Methanococcoides burtonii DSM 6242.";
RL   Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: ATP + deamido-NAD(+) + NH(3) = AMP +
CC       diphosphate + NAD(+).
CC   -!- PATHWAY: Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from
CC       deamido-NAD(+) (ammonia route): step 1/1.
CC   -!- SIMILARITY: Belongs to the NAD synthetase family.
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DR   EMBL; CP000300; ABE52695.1; -; Genomic_DNA.
DR   RefSeq; YP_566445.1; -.
DR   GeneID; 3997936; -.
DR   GenomeReviews; CP000300_GR; Mbur_1809.
DR   KEGG; mbu:Mbur_1809; -.
DR   NMPDR; fig|259564.8.peg.1724; -.
DR   HOGENOM; Q12V31; -.
DR   OMA; Q12V31; CAINPIG.
DR   BioCyc; MBUR259564:MBUR_1809-MON; -.
DR   GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR   GO; GO:0003952; F:NAD+ synthase (glutamine-hydrolyzing) activity; IEA:InterPro.
DR   GO; GO:0008795; F:NAD+ synthase activity; IEA:EC.
DR   GO; GO:0009435; P:NAD biosynthetic process; IEA:HAMAP.
DR   HAMAP; MF_00193; -; 1.
DR   InterPro; IPR003694; NAD_synthase.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   Gene3D; G3DSA:3.40.50.620; Rossmann-like_a/b/a_fold; 1.
DR   Pfam; PF02540; NAD_synthase; 1.
DR   TIGRFAMs; TIGR00552; nadE; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Ligase; NAD; Nucleotide-binding.
FT   CHAIN         1    263       NH(3)-dependent NAD(+) synthetase.
FT                                /FTId=PRO_1000077575.
FT   NP_BIND      29     36       ATP (By similarity).
FT   ACT_SITE     31     31       By similarity.
SQ   SEQUENCE   263 AA;  28578 MW;  892A88C3815431C2 CRC64;
     MDIIQAKDII IDFIGTKLEG TGIEGAVVGI SGGIDSALVA YLSVEALGAE NVLGIHMPEA
     STPKSEIEDA SKVAEALGID FKVINITNVL EVYRTAMPDI DGASAHVDGN LKARIRMSML
     YYYANMFGRV VMGTGNKSEI LLGYFTKYGD GGVDIEPIGD LYKTEVREMS KMLGVPESIL
     EKAPSAGLWE GQTDEDDLGV TYETIDKVLQ PILAGEGQER VHLKLGVPME EISSILLRVR
     SNLHKRTTPQ IAYLDDLRGD WLS
//