Q12UJ5 (GUAAA_METBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP synthase [glutamine-hydrolyzing] subunit A
EC=6.3.5.2

Alternative name(s):
Glutamine amidotransferase

Gene names

Name:	guaAA
Ordered Locus Names:	Mbur_2002

Organism

Methanococcoides burtonii (strain DSM 6242) [Complete proteome] [HAMAP]

Taxonomic identifier

259564 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanococcoides

Protein attributes

Sequence length	189 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_01510
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_01510
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_01510
Subunit structure	Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B) Potential.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 189	189	GMP synthase [glutamine-hydrolyzing] subunit A HAMAP MF_01510		PRO_0000292194
Regions
	Domain	5 – 189	185	Glutamine amidotransferase type-1
Sites
	Active site	79	1	Nucleophile By similarity
	Active site	166	1	By similarity
	Active site	168	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q12UJ5 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: B5A1D6A6925A4C4F	FASTA	189	21,080
	10 20 30 40 50 60 MKELKIIVIN NYGQFCHLIH RTVRDLDMDT KIVANTTSVE DILDEEPDGI ILSGGPSMER 70 80 90 100 110 120 VGSCQEYVES IDIPILGICL GHQLIAQTFG GHTGAGKLGG YAAIDVEVIE EDDILKGLGP 130 140 150 160 170 180 RTSVWASHAD EVTVLPDEFI HLARSDVCEI EAMRHEERPI YGVQWHPEVA HTDKGEELFM NFFKVCEDY

References

[1]

"Complete sequence of Methanococcoides burtonii DSM 6242." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Kadner K., Aerts A., Dehal P., Pitluck S., Martinez M., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Anderson I., Franzmann P., Thomas T., Saunders N., Cavicchioli R., Sowers K., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 6242.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000300 Genomic DNA. Translation: ABE52881.1.
RefSeq	YP_566631.1. NC_007955.1.
3D structure databases
ProteinModelPortal	Q12UJ5.
ModBase	Search...
Protein-protein interaction databases
STRING	Q12UJ5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3996954.
GenomeReviews	Gene locus Mbur_2002 in contig CP000300_GR.
KEGG	mbu:Mbur_2002.
NMPDR	fig|259564.8.peg.1913.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG05587.
HOGENOM	HBG292341.
OMA	GQYVHRI.
ProtClustDB	PRK00758.
Enzyme and pathway databases
BioCyc	MBUR259564:MBUR_2002-MONOMER.
Family and domain databases
HAMAP	MF_01510. GMP_synthase_A. [Tree]
InterPro	IPR017926. GATASE_1. IPR004739. GMP_synth_N. IPR023686. GMP_synthase_A. [Graphical view]
KO	K01951.
Pfam	PF00117. GATase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00888. GuaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAAA_METBU

Accession

Primary (citable) accession number: Q12UJ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	August 22, 2006
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program