ID   PYLS_METBU              Reviewed;         416 AA.
AC   Q12UB6;
DT   28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT   22-AUG-2006, sequence version 1.
DT   16-JUN-2009, entry version 18.
DE   RecName: Full=Pyrrolysyl-tRNA synthetase;
DE            EC=6.1.1.26;
DE   AltName: Full=Pyrrolysine--tRNA ligase;
DE            Short=PylRS;
GN   Name=pylS; OrderedLocusNames=Mbur_2086;
OS   Methanococcoides burtonii (strain DSM 6242).
OC   Archaea; Euryarchaeota; Methanomicrobia; Methanosarcinales;
OC   Methanosarcinaceae; Methanococcoides.
OX   NCBI_TaxID=259564;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H.,
RA   Kadner K., Aerts A., Dehal P., Pitluck S., Martinez M., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Anderson I.,
RA   Franzmann P., Thomas T., Saunders N., Cavicchioli R., Sowers K.,
RA   Richardson P.;
RT   "Complete sequence of Methanococcoides burtonii DSM 6242.";
RL   Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the attachment of pyrrolysine to tRNA(Pyl).
CC       Pyrrolysine is a lysine derivative encoded by the termination
CC       codon UAG (By similarity).
CC   -!- CATALYTIC ACTIVITY: ATP + L-pyrrolysine + tRNA(Pyl) = AMP +
CC       diphosphate + L-pyrrolysyl-tRNA(Pyl).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase
CC       family.
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DR   EMBL; CP000300; ABE52960.1; -; Genomic_DNA.
DR   RefSeq; YP_566710.1; -.
DR   GeneID; 3998168; -.
DR   GenomeReviews; CP000300_GR; Mbur_2086.
DR   KEGG; mbu:Mbur_2086; -.
DR   NMPDR; fig|259564.8.peg.1993; -.
DR   HOGENOM; Q12UB6; -.
DR   OMA; Q12UB6; SGCTREN.
DR   BioCyc; MBUR259564:MBUR_2086-MON; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004812; F:aminoacyl-tRNA ligase activity; IEA:HAMAP.
DR   GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR   GO; GO:0006418; P:tRNA aminoacylation for protein translation; IEA:HAMAP.
DR   HAMAP; MF_01573; -; 1.
DR   InterPro; IPR018150; aa-tRNA-synt_II-like.
DR   InterPro; IPR002314; aa-tRNA-synt_IIb_cons-reg.
DR   InterPro; IPR006195; aa-tRNA-synth_II_cons-reg.
DR   InterPro; IPR012739; PylS.
DR   PANTHER; PTHR22594; aa-tRNA-synt_II; 1.
DR   Pfam; PF00587; tRNA-synt_2b; 1.
DR   TIGRFAMs; TIGR02367; PylS; 1.
DR   PROSITE; PS50862; AA_TRNA_LIGASE_II; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm;
KW   Ligase; Nucleotide-binding; Protein biosynthesis.
FT   CHAIN         1    416       Pyrrolysyl-tRNA synthetase.
FT                                /FTId=PRO_0000260452.
SQ   SEQUENCE   416 AA;  48305 MW;  8A941DAA2BC5FCAA CRC64;
     MEKQLLDVLV ELNGVWLSRS GLLHGIRNFE ITTKHIHIET DCGARFTVRN SRSSRSARSL
     RHNKYRKPCK RCRPADEQID RFVKKTFKEK RQTVSVFSSP KKHVPKKPKV AVIKSFSIST
     PSPKEASVSN SIPTPSISVV KDEVKVPEVK YTPSQIERLK TLMSPDDKIP IQDELPEFKV
     LEKELIQRRR DDLKKMYEED REDRLGKLER DITEFFVDRG FLEIKSPIMI PFEYIERMGI
     DKDDHLNKQI FRVDESMCLR PMLAPCLYNY LRKLDKVLPD PIRIFEIGPC YRKESDGSSH
     LEEFTMVNFC QMGSGCTREN MEALIDEFLE HLGIEYEIEA DNCMVYGDTI DIMHGDLELS
     SAVVGPIPLD REWGVNKPWM GAGFGLERLL KVRHNYTNIR RASRSELYYN GINTNL
//