Reviewed, UniProtKB/Swiss-Prot Q12UB6 (PYLS_METBU)

Names and origin

Protein names

Recommended name:
    Pyrrolysyl-tRNA synthetase
    EC=6.1.1.26
Alternative name(s):
    Pyrrolysine--tRNA ligase
      Short name=PylRS

Gene names

Name:	pylS
Ordered Locus Names:	Mbur_2086

Organism

Methanococcoides burtonii (strain DSM 6242) [Complete proteome] [HAMAP]

Taxonomic identifier

259564 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanococcoides

Protein attributes

Sequence length	416 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of pyrrolysine to tRNA(Pyl). Pyrrolysine is a lysine derivative encoded by the termination codon UAG By similarity.
Catalytic activity	ATP + L-pyrrolysine + tRNA(Pyl) = AMP + diphosphate + L-pyrrolysyl-tRNA(Pyl). HAMAP MF_01573
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 416	416	Pyrrolysyl-tRNA synthetase HAMAP MF_01573		PRO_0000260452

Sequences

	Sequence		Length	Mass (Da)
	Q12UB6-1 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 8A941DAA2BC5FCAA	FASTA	416	48,305
	10 20 30 40 50 60 MEKQLLDVLV ELNGVWLSRS GLLHGIRNFE ITTKHIHIET DCGARFTVRN SRSSRSARSL 70 80 90 100 110 120 RHNKYRKPCK RCRPADEQID RFVKKTFKEK RQTVSVFSSP KKHVPKKPKV AVIKSFSIST 130 140 150 160 170 180 PSPKEASVSN SIPTPSISVV KDEVKVPEVK YTPSQIERLK TLMSPDDKIP IQDELPEFKV 190 200 210 220 230 240 LEKELIQRRR DDLKKMYEED REDRLGKLER DITEFFVDRG FLEIKSPIMI PFEYIERMGI 250 260 270 280 290 300 DKDDHLNKQI FRVDESMCLR PMLAPCLYNY LRKLDKVLPD PIRIFEIGPC YRKESDGSSH 310 320 330 340 350 360 LEEFTMVNFC QMGSGCTREN MEALIDEFLE HLGIEYEIEA DNCMVYGDTI DIMHGDLELS 370 380 390 400 410 SAVVGPIPLD REWGVNKPWM GAGFGLERLL KVRHNYTNIR RASRSELYYN GINTNL

References

[1]

"Complete sequence of Methanococcoides burtonii DSM 6242." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Kadner K., Aerts A., Dehal P., Pitluck S., Martinez M., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Anderson I., Franzmann P., Thomas T., Saunders N., Cavicchioli R., Sowers K., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000300 Genomic DNA. Translation: ABE52960.1.
RefSeq	YP_566710.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3998168.
GenomeReviews	Gene locus Mbur_2086 in contig CP000300_GR.
KEGG	mbu:Mbur_2086.
NMPDR	fig|259564.8.peg.1993.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q12UB6.
OMA	Q12UB6. SGCTREN.
Enzyme and pathway databases
BioCyc	MBUR259564:MBUR_2086-MON.
Family and domain databases
HAMAP	MF_01573. [Tree]
InterPro	IPR018150. aa-tRNA-synt_II-like. IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR012739. PylS. [Graphical view]
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit.
Pfam	PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
TIGRFAMs	TIGR02367. PylS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYLS_METBU

Accession

Primary (citable) accession number: Q12UB6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	August 22, 2006
Last modified:	June 16, 2009
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)