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Q12UB6 (PYLS_METBU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 34. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Pyrrolysine--tRNA ligase
EC=6.1.1.26
Alternative name(s):
Pyrrolysine--tRNA(Pyl) ligase
Pyrrolysyl-tRNA synthetase
Short name=PylRS
Gene names
Name:pylS
Ordered Locus Names:Mbur_2086
OrganismMethanococcoides burtonii (strain DSM 6242) [Complete proteome] [HAMAP]
Taxonomic identifier259564 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanococcoides

Protein attributes

Sequence length416 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of pyrrolysine to tRNA(Pyl). Pyrrolysine is a lysine derivative encoded by the termination codon UAG By similarity. HAMAP MF_01573

Catalytic activity

ATP + L-pyrrolysine + tRNA(Pyl) = AMP + diphosphate + L-pyrrolysyl-tRNA(Pyl). HAMAP MF_01573

Subcellular location

Cytoplasm By similarity HAMAP MF_01573.

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processtRNA aminoacylation for protein translation

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

aminoacyl-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 416416Pyrrolysine--tRNA ligase HAMAP MF_01573
PRO_0000260452

Sequences

Sequence LengthMass (Da)Tools
Q12UB6 [UniParc].

Last modified August 22, 2006. Version 1.
Checksum: 8A941DAA2BC5FCAA

FASTA41648,305
        10         20         30         40         50         60 
MEKQLLDVLV ELNGVWLSRS GLLHGIRNFE ITTKHIHIET DCGARFTVRN SRSSRSARSL 

        70         80         90        100        110        120 
RHNKYRKPCK RCRPADEQID RFVKKTFKEK RQTVSVFSSP KKHVPKKPKV AVIKSFSIST 

       130        140        150        160        170        180 
PSPKEASVSN SIPTPSISVV KDEVKVPEVK YTPSQIERLK TLMSPDDKIP IQDELPEFKV 

       190        200        210        220        230        240 
LEKELIQRRR DDLKKMYEED REDRLGKLER DITEFFVDRG FLEIKSPIMI PFEYIERMGI 

       250        260        270        280        290        300 
DKDDHLNKQI FRVDESMCLR PMLAPCLYNY LRKLDKVLPD PIRIFEIGPC YRKESDGSSH 

       310        320        330        340        350        360 
LEEFTMVNFC QMGSGCTREN MEALIDEFLE HLGIEYEIEA DNCMVYGDTI DIMHGDLELS 

       370        380        390        400        410 
SAVVGPIPLD REWGVNKPWM GAGFGLERLL KVRHNYTNIR RASRSELYYN GINTNL

« Hide

References

[1]"Complete sequence of Methanococcoides burtonii DSM 6242."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Kadner K., Aerts A., Dehal P., Pitluck S., Martinez M., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Anderson I., Franzmann P., Thomas T., Saunders N., Cavicchioli R., Sowers K., Richardson P.
Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 6242.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000300 Genomic DNA. Translation: ABE52960.1.
RefSeqYP_566710.1. NC_007955.1.

3D structure databases

ProteinModelPortalQ12UB6.
SMRQ12UB6. Positions 152-416.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ12UB6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3998168.
GenomeReviewsGene locus Mbur_2086 in contig CP000300_GR.
KEGGmbu:Mbur_2086.
NMPDRfig|259564.8.peg.1993.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGarNOG05099.
HOGENOMHBG459849.
OMAFCQMGSG.
ProtClustDBPRK09537.

Enzyme and pathway databases

BioCycMBUR259564:MBUR_2086-MONOMER.

Family and domain databases

HAMAPMF_01573. Pyl_tRNA_synth.
[Tree]
InterProIPR018150. aa-tRNA-synt_II-like.
IPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR012739. Pyrrolysyl-tRNA_ligase.
IPR023877. Pyrrolysyl-tRNA_ligase_C.
IPR023878. Pyrrolysyl-tRNA_ligase_N.
[Graphical view]
KOK11627.
PANTHERPTHR22594. aa-tRNA-synt_II. 1 hit.
PfamPF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
TIGRFAMsTIGR02367. PylS_Cterm. 1 hit.
TIGR03912. PylS_Nterm. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePYLS_METBU
AccessionPrimary (citable) accession number: Q12UB6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: August 22, 2006
Last modified: January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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