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Q12U32 (Q12U32_METBU) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Amidophosphoribosyltransferase PIRNR PIRNR000485
Short name=ATase PIRNR PIRNR000485
EC=2.4.2.14 PIRNR PIRNR000485
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase PIRNR PIRNR000485
Gene names
Name:purF EMBL ABE53044.1
Ordered Locus Names:Mbur_2179
OrganismMethanococcoides burtonii (strain DSM 6242) [Complete proteome] [HAMAP]
Taxonomic identifier259564 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanococcoides

Protein attributes

Sequence length462 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O. PIRNR PIRNR000485

Cofactor

Binds 1 4Fe-4S cluster per subunit By similarity. PIRSR PIRSR000485-3

Binds 1 magnesium ion per subunit By similarity. PIRNR PIRNR000485

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2. PIRNR PIRNR000485

Sequence similarities

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. PIRNR PIRNR000485

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site51For GATase activity By similarity PIRSR PIRSR000485-1
Metal binding2461Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
Metal binding3921Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
Metal binding4431Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
Metal binding4461Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3

Sequences

Sequence LengthMass (Da)Tools
Q12U32 [UniParc].

Last modified August 22, 2006. Version 1.
Checksum: 466F9B3E6C08BA0D

FASTA46250,897
        10         20         30         40         50         60 
MKEECGVVGV LMHKADTKAK PASLQIYYAL YALQHRGQES TGITVKNGDK LKSIKGMGLV 

        70         80         90        100        110        120 
PEVYSKDELL KLEGRLGVGH VRYSTTGDSN IVNCQPLMVN YKNGNLAIAH NGNLVNGQEL 

       130        140        150        160        170        180 
RDELESEGRI FITSSDTEVI AHLLVKALLI HDPLESIKEV MSMLKGSYSL AIMIDDRLFA 

       190        200        210        220        230        240 
VRDPLGFKPL CVGEIDGGYV VASESVAIDT LNGKLTRDVK AGEVVEITEN GFEGHQMLTE 

       250        260        270        280        290        300 
KNCAHCVFEY IYFARPDSII DGQLVYKVRE QIGRELAKEH PVDADIVSPV PDSGITSAIG 

       310        320        330        340        350        360 
YSDESGINYR EGLMKNRYIG RTFILPGQEM RETAVRLKMN TISENLRDRK VIILDDSIVR 

       370        380        390        400        410        420 
GTTSRRIIEM VRNAGAKEVH ARIGSPAIIA PCYMGIDMAS REELIAAKKP LVDVKNAITA 

       430        440        450        460 
DSLGYLSIEG LIKSIGIHRD ELCLGCLTER YPIKIVGEKC SR

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References

[1]"Complete sequence of Methanococcoides burtonii DSM 6242."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Kadner K., Aerts A., Dehal P., Pitluck S., Martinez M., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Anderson I., Franzmann P., Thomas T., Saunders N., Cavicchioli R., Sowers K., Richardson P.
Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 6242.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000300 Genomic DNA. Translation: ABE53044.1.
RefSeqYP_566794.1. NC_007955.1.

3D structure databases

ProteinModelPortalQ12U32.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ12U32.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3997035.
GenomeReviewsGene locus Mbur_2179 in contig CP000300_GR.
KEGGmbu:Mbur_2179.
NMPDRfig|259564.8.peg.2084.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGarNOG04070.
HOGENOMHBG392416.
OMADSMFEDQ.
PhylomeDBQ12U32.
ProtClustDBCLSK811961.

Family and domain databases

InterProIPR005854. Amd_phspho_trans.
IPR000583. GATase_2.
IPR017932. GATase_II.
IPR000836. PRibTrfase.
[Graphical view]
KOK00764.
PANTHERPTHR11907. Amd_phspho_trans. 1 hit.
PfamPF00310. GATase_2. 2 hits.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFPIRSF000485. Amd_phspho_trans. 1 hit.
TIGRFAMsTIGR01134. PurF. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameQ12U32_METBU
AccessionPrimary (citable) accession number: Q12U32
Entry history
Integrated into UniProtKB/TrEMBL: August 22, 2006
Last sequence update: August 22, 2006
Last modified: January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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