Q12U25 (SYA_METBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine--tRNA ligase
EC=6.1.1.7

Alternative name(s):
Alanyl-tRNA synthetase
Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	Mbur_2186

Organism

Methanococcoides burtonii (strain DSM 6242) [Complete proteome] [HAMAP]

Taxonomic identifier

259564 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanococcoides

Protein attributes

Sequence length	923 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036_A
Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036_A
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036_A
Subcellular location	Cytoplasm By similarity HAMAP MF_00036_A.
Domain	Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036_A
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alanine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 923	923	Alanine--tRNA ligase HAMAP MF_00036_A		PRO_0000347885
Sites
	Metal binding	611	1	Zinc By similarity
	Metal binding	615	1	Zinc By similarity
	Metal binding	714	1	Zinc By similarity
	Metal binding	718	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q12U25 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: B674C89E48E056C9	FASTA	923	103,461
	10 20 30 40 50 60 MLEDEYQIDF FSDNGFVRKQ CPTCGNFFWT RDIERSTCGD APCDPYSFIG NPVFKKELEL 70 80 90 100 110 120 PDMREFYLNF FEEQGHTRIE RYPVIARWRD DIYLTIASIA DFQPFVTSGQ VPPPANPLTI 130 140 150 160 170 180 SQPCIRLSDL DAVGKSGRHL TTFEMMAHHA FNTKNEEIYW KEHTLELCDG LLNSLGADPM 190 200 210 220 230 240 AVTYKEEPWA GGGNAGACVE VLIGGLEVAT LVFMNLKKDK NGDIDIKGDM YSKMENYIVD 250 260 270 280 290 300 TGYGLERLVW ASKGSPTIYD AIFPSIVNEL MGLAGIEHEL ENNEYSHILS QNARLAGLMD 310 320 330 340 350 360 ISEKANLLEL RKQVAASIGI TADKLSSIME PVENVYAIAD HTRCLTFMIG DGIIPSNVKA 370 380 390 400 410 420 GYLARLVIRR TLRMMKDLGI MIPISEIIQM HINNLPEYPE FQKRFDVIKD ILEHEERKFA 430 440 450 460 470 480 ETLERGRRMM EKSARHYKES GEKMPLETII DMYDSHGIPP EISKAVASDV GVEVDLPDNF 490 500 510 520 530 540 YSLVADKHSQ SEEKEEKVVP FADKIARLPK TKRLFYDEPN RMDFDAVVLE VFDNHIVLDN 550 560 570 580 590 600 TLMYPEGGGQ PADHGTLTVE DVVLKVVDTQ MYDGVVVHTI NEIEDELHIR KGDMVVGRVN 610 620 630 640 650 660 EKRRMAHARH HTATHIINDA AREVLGSHIW QTGAQKFADR ARLDISHYKR ITQEEANQIE 670 680 690 700 710 720 IIANHTVMKN KRIISDWMDR TEAEQKYGFR LYQGGVPPGK MIRVLQVGND IEACAGTHCT 730 740 750 760 770 780 NTGLVGPIKI LKTERIQDGV ERLEYAAGEA AIIAMQDIET LVRDSSETLR VSAEQLPSTI 790 800 810 820 830 840 ERFFDEWKEL KKENNKLKEE LAHSRVSQLV NDAEDVNGIR IITKAIPHAD SEELTKTAGE 850 860 870 880 890 900 LTQESNVVAI LISEMDGVKI VATAGDDAVK RGVNVGAIVK EMSTMVGGGG GGRPNMARGG 910 920 GTDPSGMDNA LSRSVELLKE QLN

References

[1]

"Complete sequence of Methanococcoides burtonii DSM 6242." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Kadner K., Aerts A., Dehal P., Pitluck S., Martinez M., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Anderson I., Franzmann P., Thomas T., Saunders N., Cavicchioli R., Sowers K., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 6242.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000300 Genomic DNA. Translation: ABE53051.1.
RefSeq	YP_566801.1. NC_007955.1.
3D structure databases
ProteinModelPortal	Q12U25.
ModBase	Search...
Protein-protein interaction databases
STRING	Q12U25.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3997042.
GenomeReviews	Gene locus Mbur_2186 in contig CP000300_GR.
KEGG	mbu:Mbur_2186.
NMPDR	fig|259564.8.peg.2090.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04130.
HOGENOM	HBG392147.
OMA	MFTNSGM.
PhylomeDB	Q12U25.
ProtClustDB	PRK13902.
Enzyme and pathway databases
BioCyc	MBUR259564:MBUR_2186-MONOMER.
Family and domain databases
HAMAP	MF_00036_A. Ala_tRNA_synth_A. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR022429. Ala-tRNA_synth_arc. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
KO	K01872.
PANTHER	PTHR11777:SF6. PTHR11777:SF6. 1 hit.
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR03683. A-tRNA_syn_arch. 1 hit. TIGR00344. AlaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_METBU

Accession

Primary (citable) accession number: Q12U25

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	August 22, 2006
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program