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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Methanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 / ACE-M)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMBUR259564:GHBZ-2377-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:Mbur_2335
OrganismiMethanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 / ACE-M)
Taxonomic identifieri259564 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanococcoides
ProteomesiUP000001979 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 569569Glutamate--tRNA ligasePRO_1000001922Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi259564.Mbur_2335.

Structurei

3D structure databases

ProteinModelPortaliQ12TN7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi110 – 12011"HIGH" regionAdd
BLAST

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000073585.
KOiK01885.
OMAiGEYSGWD.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_A. Glu_tRNA_synth_A.
InterProiIPR004526. Glu-tRNA-synth_arc/euk.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR022888. Glu_tRNA_synth_arc.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF50715. SSF50715. 1 hit.
TIGRFAMsiTIGR00463. gltX_arch. 1 hit.

Sequencei

Sequence statusi: Complete.

Q12TN7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLSDEDKIT IEKFALQNAV KYGKAPQLGA VMGKVMGLCP HLRPLSKEVG
60 70 80 90 100
PVIQHVLDEV MKEVPEEWQA RLEVIAPELI EELNTKKEPD KGLKPLDVKE
110 120 130 140 150
GESVVMRFAP NPNGPPTLGS TRGIVVNSEY VKRYGGKFII RFDDTDPQTK
160 170 180 190 200
RPMLEAYDWY LEDCEWLDAK PDEVVIASDN MEVYYDYARQ LLEMGHAYVC
210 220 230 240 250
FCEGGDFKKF KDAKEPCPHR GHAPEVNLEH WDKMLAGEYE EKAAVVRIKT
260 270 280 290 300
DIEHKDPAMR DFGAFRIVKT AHPRPEVDDK YVVWPLLDFE GAIEDHVLGM
310 320 330 340 350
THIIRGKDLM DSEKRQGYIY NYLGWEYPKT THWGRVKMHE FGKFSTSGLR
360 370 380 390 400
QSIEDGEYSG WDDPRLPTLR ALRRRGIKPE AIRKFMIDMG VGETDVSLSM
410 420 430 440 450
DTLYAENRKI IDTIANRYFF VWDPVELEVE DAVCCTVNPS LHPSEDRGVR
460 470 480 490 500
CIDIGSKLLV CKSDVESAEL GDMLRMKYLY NIEITSVSPL KCKCIGDSME
510 520 530 540 550
DAKSNKMRII HWVPEDGIPV KVLAPQGEFI GMGEKQIVDE LDNVVQFERF
560
GFCRIDSVDD GVVAYFTHK
Length:569
Mass (Da):64,987
Last modified:August 22, 2006 - v1
Checksum:i09410882AAD3C7D3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000300 Genomic DNA. Translation: ABE53189.1.
RefSeqiWP_011500324.1. NC_007955.1.
YP_566939.1. NC_007955.1.

Genome annotation databases

EnsemblBacteriaiABE53189; ABE53189; Mbur_2335.
GeneIDi3998916.
KEGGimbu:Mbur_2335.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000300 Genomic DNA. Translation: ABE53189.1.
RefSeqiWP_011500324.1. NC_007955.1.
YP_566939.1. NC_007955.1.

3D structure databases

ProteinModelPortaliQ12TN7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi259564.Mbur_2335.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABE53189; ABE53189; Mbur_2335.
GeneIDi3998916.
KEGGimbu:Mbur_2335.

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000073585.
KOiK01885.
OMAiGEYSGWD.

Enzyme and pathway databases

BioCyciMBUR259564:GHBZ-2377-MONOMER.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_A. Glu_tRNA_synth_A.
InterProiIPR004526. Glu-tRNA-synth_arc/euk.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR022888. Glu_tRNA_synth_arc.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF50715. SSF50715. 1 hit.
TIGRFAMsiTIGR00463. gltX_arch. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 6242 / NBRC 107633 / OCM 468 / ACE-M.

Entry informationi

Entry nameiSYE_METBU
AccessioniPrimary (citable) accession number: Q12TN7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 22, 2006
Last modified: April 1, 2015
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.