Q12TG0 (PROB_METBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate 5-kinase
EC=2.7.2.11

Alternative name(s):
Gamma-glutamyl kinase
Short name=GK

Gene names

Name:	proB
Ordered Locus Names:	Mbur_2415

Organism

Methanococcoides burtonii (strain DSM 6242) [Complete proteome] [HAMAP]

Taxonomic identifier

259564 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanococcoides

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline. HAMAP MF_00456
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP MF_00456
Subcellular location	Cytoplasm By similarity HAMAP MF_00456.
Sequence similarities	Belongs to the glutamate 5-kinase family. Contains 1 PUA domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro glutamate 5-kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 375	375	Glutamate 5-kinase HAMAP MF_00456		PRO_0000253016
Regions
	Domain	284 – 360	77	PUA

Sequences

	Sequence		Length	Mass (Da)
	Q12TG0 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 756DF00BC60F0CD4	FASTA	375	40,967
	10 20 30 40 50 60 MTNRKEIIGD AEKIVIKIGT TSISREDGSL NNEFMDTIAS QVSELHRAGK QIILVSSGSI 70 80 90 100 110 120 GIGIEILDLG CRPKEIPVRQ AAAAVGQGVL MQHWTEAFQK YGLNVAQILL TYDSFTNRLT 130 140 150 160 170 180 YLNLRNSIST LLSYGVIPII NENDPICVHE IEATLGDNDK LSAMVASKME ADLLILFTDI 190 200 210 220 230 240 DGLYDKNPKR HDDAVLLRTV EEITPTIESY GGNPTSMKGV GGMRTKIDAA KICNISGCYM 250 260 270 280 290 300 VIANSNVDDG IRRILDGEEL GTLFLTNQFV HKNRIRWIIL ARSSGSIVVD TGAKEALAKR 310 320 330 340 350 360 MSLLPSGVLG VAGTFDRGDI VKLECDGVVF GKGITDYTSE ELKAIKGKQT NEIADILGYK 370 NYDHVVQKDN IGLFK

References

[1]

"Complete sequence of Methanococcoides burtonii DSM 6242." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Kadner K., Aerts A., Dehal P., Pitluck S., Martinez M., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Anderson I., Franzmann P., Thomas T., Saunders N., Cavicchioli R., Sowers K., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 6242.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000300 Genomic DNA. Translation: ABE53266.1.
RefSeq	YP_567016.1. NC_007955.1.
3D structure databases
ProteinModelPortal	Q12TG0.
ModBase	Search...
Protein-protein interaction databases
STRING	Q12TG0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3999005.
GenomeReviews	Gene locus Mbur_2415 in contig CP000300_GR.
KEGG	mbu:Mbur_2415.
NMPDR	fig|259564.8.peg.2319.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG05522.
HOGENOM	HBG507643.
OMA	TFGDNDM.
PhylomeDB	Q12TG0.
ProtClustDB	PRK05429.
Enzyme and pathway databases
BioCyc	MBUR259564:MBUR_2415-MONOMER.
Family and domain databases
HAMAP	MF_00456. ProB. [Tree]
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu/AcGlu_kinase. IPR011529. Glu_5kinase. IPR005715. Glu_5kinase/COase_Synthase. IPR019797. Glutamate_5-kinase_CS. IPR002478. PUA. IPR015947. PUA-like_domain. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
KO	K00931.
Pfam	PF00696. AA_kinase. 1 hit. PF01472. PUA. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SMART	SM00359. PUA. 1 hit. [Graphical view]
SUPFAM	SSF53633. Aa_kinase. 1 hit. SSF88697. PUA-like. 1 hit.
TIGRFAMs	TIGR01027. ProB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. PS50890. PUA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB_METBU

Accession

Primary (citable) accession number: Q12TG0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 17, 2006
Last sequence update:	August 22, 2006
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program