Q12TF9 (PROA_METBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-glutamyl phosphate reductase
Short name=GPR
EC=1.2.1.41

Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	Mbur_2416

Organism

Methanococcoides burtonii (strain DSM 6242) [Complete proteome] [HAMAP]

Taxonomic identifier

259564 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanococcoides

Protein attributes

Sequence length	449 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate. HAMAP MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity HAMAP MF_00412.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 449	449	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_1000049963

Sequences

	Sequence		Length	Mass (Da)
	Q12TF9 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: C5A2E1B00FACE5FC	FASTA	449	49,610
	10 20 30 40 50 60 MATDIEQKVM EAKMASIVLA SVDTQTKDNA LEAMAKALDA NRNKILEANK ADLEEAERMK 70 80 90 100 110 120 NEGKLSQALV DRLKVTDPKI DGMISGIRDV IKLEDPSGRT INTLELDKGL ELYQVSSPIG 130 140 150 160 170 180 LIGVIFESRP DVVPQVMSLC LKSGNATVFK GGSEALNSNR VIFNILVEAL EDTPGIPKGA 190 200 210 220 230 240 FQLMETREEV MDILALDEYI DLLIPRGSND FVKFIQDNTK ISVLGHADGI CHVYVDTNAD 250 260 270 280 290 300 LNKAYDVCFD SKVQYPAVCN AMETLLINRE IAEEFLPEMV RRYEEVGVEL RFDEGSYAIA 310 320 330 340 350 360 EKLGSANIAK ATEDDWKTEY NDFILSIKLV DSIEEAIDHI NKYGSHHTDA IITENKTKRK 370 380 390 400 410 420 QFIALVDSSS VMVNASTRFA DGFRYGKGAE VGISTNKIHA RGPVGMEGLV IYKYVLLGNG 430 440 DKVATYAGDT PRPFTHKELD SKLSDIINE

References

[1]

"Complete sequence of Methanococcoides burtonii DSM 6242." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Kadner K., Aerts A., Dehal P., Pitluck S., Martinez M., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Anderson I., Franzmann P., Thomas T., Saunders N., Cavicchioli R., Sowers K., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 6242.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000300 Genomic DNA. Translation: ABE53267.1.
RefSeq	YP_567017.1. NC_007955.1.
3D structure databases
ProteinModelPortal	Q12TF9.
ModBase	Search...
Protein-protein interaction databases
STRING	Q12TF9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3999006.
GenomeReviews	Gene locus Mbur_2416 in contig CP000300_GR.
KEGG	mbu:Mbur_2416.
NMPDR	fig|259564.8.peg.2320.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG05521.
HOGENOM	HBG318080.
OMA	QYPAACN.
PhylomeDB	Q12TF9.
ProtClustDB	PRK00197.
Enzyme and pathway databases
BioCyc	MBUR259564:MBUR_2416-MONOMER.
Family and domain databases
HAMAP	MF_00412. ProA. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR000965. G-glutamylP_reductase. IPR020593. G-glutamylP_reductase_CS. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 2 hits.
KO	K00147.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR00407. ProA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_METBU

Accession

Primary (citable) accession number: Q12TF9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 22, 2006
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program