Reviewed, UniProtKB/Swiss-Prot Q12TF2 (DP2L_METBU)

Names and origin

Protein names

Recommended name:
    DNA polymerase II large subunit
      Short name=Pol II
    EC=2.7.7.7

Gene names

Name:	polC
Ordered Locus Names:	Mbur_2423

Organism

Methanococcoides burtonii (strain DSM 6242) [Complete proteome] [HAMAP]

Taxonomic identifier

259564 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanococcoides

Protein attributes

Sequence length	1145 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates.
Subunit structure	Heterodimer of a large subunit and a small subunit By similarity.
Sequence similarities	Belongs to the archaeal DNA polymerase II family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	DNA-binding
Molecular function	DNA-directed DNA polymerase Exonuclease Hydrolase Nuclease Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	DNA catabolic process, exonucleolytic Inferred from electronic annotation. Source: HAMAP DNA-dependent DNA replication Inferred from electronic annotation. Source: HAMAP
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: HAMAP DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1145	1145	DNA polymerase II large subunit		PRO_0000294685

Sequences

	Sequence		Length	Mass (Da)
	Q12TF2-1 [UniParc]. Last modified July 10, 2007. Version 2. Checksum: 2E4E696ABF9D18E4	FASTA	1,145	127,582
	10 20 30 40 50 60 MAEIVASDEM NEYFNTLEGT LKKEIDIVND ARSRGKDPKP HVEIPLAKDL ADRVENLIGV 70 80 90 100 110 120 KGVAELIRKL EETMSREEAA LALGREVAQG KVGEFDSKSE AIEAAIRVSV AMLTEGVVAA 130 140 150 160 170 180 PIEGIDRASI GKNDDGSEYV SIFYAGPIRS AGGTAQALSV LVGDYVRRGV GIDRYKPRKE 190 200 210 220 230 240 EVERYIEEIM LYKRVASLQY TPSEEEIRLI VENCPICIDG EPTEAEEVEG HRNLERIDTN 250 260 270 280 290 300 RVRGGMALVL AEGLALKAPK IQKHVKNLKI DGWEWLEQLI SGVKSSSESD EDEETDGKPK 310 320 330 340 350 360 IKPKDKYMRD LIAGRPVFSH PSRPGGFRLR YGRSRNTSFA AAGISPAGMI VMDDFIAPGT 370 380 390 400 410 420 QLKVERPGKA AGMAPVDSIE GPTVRLNNGD VIRIDTIDEA YALRSEVEEI IDIGEILINY 430 440 450 460 470 480 GDFLENNHPL APSPYCFEWW IQEYRKAAPD TETNEAELKE PTQEVALDLC KELNIPLHPK 490 500 510 520 530 540 FTYLWHDIDN SQYTALADLI SKDGLLEADG SFLKLPLQRT IDTGMKKVLE DLLVQHKIQG 550 560 570 580 590 600 QALTIEEPLP LIHSLGLDEE LSKGWDSLGH EELLENINEI AGFVVRPRAP TRIGARMGRP 610 620 630 640 650 660 EKSDKRKMTP APHALFPIAE AGGNTRSLEK AANFKVNTNS KAGTIPVEIG NRICPACGVE 670 680 690 700 710 720 GFEFRCECGE YTLPKLFCPR CGISVNKEKC PKCNSKTTCT SMRKIDFKSI YQKAFESIGE 730 740 750 760 770 780 RDHLDSFKGV KKMMSKHMTP EPLEKGILRA KHGLFTFKDG TVRYDMSDIP LTHIRPAEIG 790 800 810 820 830 840 VSCERMIELG YLKDIYGKPL IDSEQVLCLK VQDLVISYDA ADYILRITQY IDDLLVKYYK 850 860 870 880 890 900 VAPYYNAKHI DDIVGVLLMG LAPHTSAGVL GRLIGFTTAS VGYAHPYFHA AKRRNCDGDE 910 920 930 940 950 960 DCVMLLMDGL LNFSRDYLPD KRGGQMDAPL VLTTRLDPSE VDKEAHNIDM CASYPLEFYE 970 980 990 1000 1010 1020 ATQNIANPKD FEGTMDLVSG RLGTTLQYEE FMFTHDTSNI AAGPLKSAYK TLGTMVEKMD 1030 1040 1050 1060 1070 1080 AQLELAKKIR AVDAPDVAER VLTSHFLPDM FGNLRAFSRQ RTRCVKCAAK FRRPPLTGSC 1090 1100 1110 1120 1130 1140 PKCGGRVILT VHEGAVKKYL QVSIKIAEEY NVSSYTKQRI ELIGYDMKSL FENDKSKQMG LSDFM

References

[1]

"Complete sequence of Methanococcoides burtonii DSM 6242." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Kadner K., Aerts A., Dehal P., Pitluck S., Martinez M., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Anderson I., Franzmann P., Thomas T., Saunders N., Cavicchioli R., Sowers K., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000300 Genomic DNA. Translation: ABE53274.1. Different initiation.
RefSeq	YP_567024.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3999013.
GenomeReviews	Gene locus Mbur_2423 in contig CP000300_GR.
KEGG	mbu:Mbur_2423.
NMPDR	fig|259564.8.peg.2327.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q12TF2.
Enzyme and pathway databases
BioCyc	MBUR259564:MBUR_2423-MON.
Family and domain databases
HAMAP	MF_00324. [Tree]
InterPro	IPR004475. PolC_DP2. IPR016033. PolC_DP2_N. [Graphical view]
Pfam	PF03833. PolC_DP2. 1 hit. [Graphical view]
PIRSF	PIRSF016275. PolC_DP2. 1 hit.
TIGRFAMs	TIGR00354. polC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DP2L_METBU

Accession

Primary (citable) accession number: Q12TF2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	July 10, 2007
Last modified:	July 22, 2008
This is version 16 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)