Q12T32 (RLMF_SHEDO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase F
EC=2.1.1.181

Alternative name(s):
23S rRNA mA1618 methyltransferase
rRNA adenine N-6-methyltransferase

Gene names

Name:	rlmF
Ordered Locus Names:	Sden_0097

Organism

Shewanella denitrificans (strain OS217 / ATCC BAA-1090 / DSM 15013) [Complete proteome] [HAMAP]

Taxonomic identifier

318161 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the adenine in position 1618 of 23S rRNA By similarity. HAMAP MF_01848
Catalytic activity	S-adenosyl-L-methionine + adenine(1618) in 23S rRNA = S-adenosyl-L-homocysteine + rRNA containing N(6)-methyladenine(1618) in 23S rRNA. HAMAP MF_01848
Subcellular location	Cytoplasm Potential HAMAP MF_01848.
Sequence similarities	Belongs to the methyltransferase superfamily. METTL16/rlmF family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	rRNA (adenine-N6-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 360	360	Ribosomal RNA large subunit methyltransferase F HAMAP MF_01848		PRO_0000349954

Sequences

	Sequence		Length	Mass (Da)
	Q12T32 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: C67F79419E7B55C1	FASTA	360	39,531
	10 20 30 40 50 60 MSKLISKQGK RPALSQSGLA KPSTSKKSSA SKNANTETAK LLTKALHPRN VHKYGYDFAA 70 80 90 100 110 120 LSKTLPALTP FVGPNAYGNI SIDFANPDAV KQLNAALLLH HYSIRDWDIP HGYLCPPIPG 130 140 150 160 170 180 RVDYLHYLAD LLSAPHKGKA NRNIRALDIG TGANGIYPLL GIESYSWQFV ASDIDKVSLD 190 200 210 220 230 240 NVDEILAKNP QLAAKLSLRL QLNPKAIFNG VIEKDEYFDV SLCNPPFHRS LADANAGTQR 250 260 270 280 290 300 KLSNLAKNRG QSQQQAVQAK VALNFGGQKA ELWCEGGEAA FLANMISESK GFAAQCLWFT 310 320 330 340 350 360 SLVSKSENLK PCYAQLAKQG TSEVKTIEMH QGNKITRILA WSYLSLSQRQ AWAKLRDQSR

References

[1]

"Complete sequence of Shewanella denitrificans OS217." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: OS217 / ATCC BAA-1090 / DSM 15013.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000302 Genomic DNA. Translation: ABE53394.1.
RefSeq	YP_561117.1. NC_007954.1.
3D structure databases
ProteinModelPortal	Q12T32.
ModBase	Search...
Protein-protein interaction databases
STRING	Q12T32.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4017161.
GenomeReviews	Gene locus Sden_0097 in contig CP000302_GR.
KEGG	sdn:Sden_0097.
NMPDR	fig|318161.14.peg.90.
PATRIC	23485752. VBISheDen79529_0102.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3129.
HOGENOM	HBG747880.
OMA	DFANPEA.
PhylomeDB	Q12T32.
ProtClustDB	PRK11727.
Enzyme and pathway databases
BioCyc	SDEN318161:SDEN_0097-MONOMER.
Family and domain databases
HAMAP	MF_01848. 23SrRNA_methyltr_F. [Tree]
InterPro	IPR016909. rRNA_lsu_methyltransferase_F. IPR010286. SAM-Mtase_prd. [Graphical view]
KO	K06970.
PANTHER	PTHR13393. DUF890. 1 hit.
Pfam	PF05971. Methyltransf_10. 1 hit. [Graphical view]
PIRSF	PIRSF029038. Mtase_YbiN_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMF_SHEDO

Accession

Primary (citable) accession number: Q12T32

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	August 22, 2006
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program