Reviewed, UniProtKB/Swiss-Prot Q12RU3 (TPMT_SHEDO)

Names and origin

Protein names

Recommended name:
    Thiopurine S-methyltransferase
    EC=2.1.1.67
Alternative name(s):
    Thiopurine methyltransferase

Gene names

Name:	tpm
Ordered Locus Names:	Sden_0541

Organism

Shewanella denitrificans (strain OS217 / ATCC BAA-1090 / DSM 15013) [Complete proteome] [HAMAP]

Taxonomic identifier

318161 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	218 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	S-adenosyl-L-methionine + a thiopurine = S-adenosyl-L-homocysteine + a thiopurine S-methylether. HAMAP MF_00812
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. TPMT family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thiopurine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 218	218	Thiopurine S-methyltransferase HAMAP MF_00812		PRO_1000047219
Sites
	Binding site	10	1	S-adenosyl-L-methionine By similarity
	Binding site	45	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	66	1	S-adenosyl-L-methionine By similarity
	Binding site	123	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q12RU3-1 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 76F33423FE7CEFA7	FASTA	218	25,076
	10 20 30 40 50 60 MEPNFWHDKW HQQLIGFHQA SVNALLLTHW QQLEIKPQGQ VFVPLCGKSL DMCYLAELGH 70 80 90 100 110 120 NVLGCELNLS AVEQFYAENQ LTVTCEPIGE HQFFDCEQVQ IWQGDIFTLA PKITQDISGF 130 140 150 160 170 180 YDRAALIAWP ESLRQAYVKQ LAKLIPQGCR GLLVTLDYPQ ETLQGPPFAV SPTWVETHLS 190 200 210 EYFDITLLAC RDVLDDNPRF IKKDVPWLNE SAYLLKRK

References

[1]

"Complete sequence of Shewanella denitrificans OS217." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000302 Genomic DNA. Translation: ABE53833.1.
RefSeq	YP_561556.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q12RU3.
Genome annotation databases
GeneID	4016875.
GenomeReviews	Gene locus Sden_0541 in contig CP000302_GR.
KEGG	sdn:Sden_0541.
NMPDR	fig|318161.14.peg.523.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q12RU3.
OMA	PPFAVSP.
Enzyme and pathway databases
BioCyc	SDEN318161:SDEN_0541-MON.
Family and domain databases
HAMAP	MF_00812. [Tree]
InterPro	IPR008854. Thiopurine_S-MeTrfase. IPR016822. Thiopurine_S-MeTrfase_sub. [Graphical view]
Pfam	PF05724. TPMT. 1 hit. [Graphical view]
PIRSF	PIRSF023956. Thiopurine_S-methyltransferase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TPMT_SHEDO

Accession

Primary (citable) accession number: Q12RU3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 22, 2006
Last modified:	November 3, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)