ID   MAO1_SHEDO              Reviewed;         562 AA.
AC   Q12RA0;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   22-AUG-2006, sequence version 1.
DT   16-JUN-2009, entry version 31.
DE   RecName: Full=NAD-dependent malic enzyme;
DE            Short=NAD-ME;
DE            EC=1.1.1.38;
GN   Name=sfcA; OrderedLocusNames=Sden_0736;
OS   Shewanella denitrificans (strain OS217 / ATCC BAA-1090 / DSM 15013).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC   Shewanellaceae; Shewanella.
OX   NCBI_TaxID=318161;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H.,
RA   Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H.,
RA   Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A.,
RA   Richardson P.;
RT   "Complete sequence of Shewanella denitrificans OS217.";
RL   Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: (S)-malate + NAD(+) = pyruvate + CO(2) + NADH.
CC   -!- COFACTOR: Divalent metal cations. Prefers magnesium or manganese
CC       (By similarity).
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SIMILARITY: Belongs to the malic enzymes family.
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DR   EMBL; CP000302; ABE54026.1; -; Genomic_DNA.
DR   RefSeq; YP_561749.1; -.
DR   GeneID; 4016842; -.
DR   GenomeReviews; CP000302_GR; Sden_0736.
DR   KEGG; sdn:Sden_0736; -.
DR   NMPDR; fig|318161.14.peg.718; -.
DR   HOGENOM; Q12RA0; -.
DR   OMA; Q12RA0; IQDNNET.
DR   BioCyc; SDEN318161:SDEN_0736-MON; -.
DR   GO; GO:0016619; F:malate dehydrogenase (oxaloacetate-decarbox...; IEA:HAMAP.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0051287; F:NAD or NADH binding; IEA:InterPro.
DR   GO; GO:0006108; P:malate metabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_01619; -; 1.
DR   InterPro; IPR015884; Malic_enzyme_CS.
DR   InterPro; IPR012301; Malic_N.
DR   InterPro; IPR012302; Malic_NAD_bd.
DR   InterPro; IPR001891; Malic_OxRdtase.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF00390; malic; 1.
DR   Pfam; PF03949; Malic_M; 1.
DR   PRINTS; PR00072; MALOXRDTASE.
DR   PROSITE; PS00331; MALIC_ENZYMES; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Metal-binding; NAD; Oxidoreductase.
FT   CHAIN         1    562       NAD-dependent malic enzyme.
FT                                /FTId=PRO_1000069541.
FT   ACT_SITE    101    101       Proton donor (By similarity).
FT   ACT_SITE    172    172       Proton acceptor (By similarity).
FT   METAL       243    243       Divalent metal cation (By similarity).
FT   METAL       244    244       Divalent metal cation (By similarity).
FT   METAL       267    267       Divalent metal cation (By similarity).
FT   BINDING     154    154       NAD (By similarity).
FT   BINDING     267    267       NAD (By similarity).
FT   BINDING     415    415       NAD (By similarity).
FT   SITE        267    267       Important for activity (By similarity).
SQ   SEQUENCE   562 AA;  62265 MW;  942252D30AF25265 CRC64;
     MDDTKRPLYL PFAGPAILEA PLINKGSAFS EEERIFFNLE GLLPYVIETI EEQAARAYAQ
     FSNFSNDLDK HIYLRNIQDT NETLFYRLVR NNITEMMPII YTPTVGLACE RFSKNYRRNR
     GLFISYSNKD RIDDILNNST RHKVKVIVVT DGERILGLGD QGIGGMGIPI GKLSLYTSCG
     GISPAYTLPI TLDVGTDNQE LLDDPMYMGW RHRRIEGQDY ADFVEAFMEA VHRRWPDVLI
     QFEDFAQRNA MPLLERYKDQ YCCFNDDIQG TAAVTVGSLL AASKAANTQL SKQRVVFLGA
     GSAGCGIAEA IVAQMISEGI SEAQARSQVF MVDRLGLLQD NTANLLPFQQ KLAQSSATVS
     QWHIEGDSVS LLNVINNAKP TVLIGVSGSP GQFTEAVIRA MHLHCPRPIV FPLSNPTSRV
     EATPEDVLNW TNGQALVATG SPFEPVTIGD ETFEIAQCNN SFIFPGIGLG VLACRAKRVS
     DEMLMASSRA LAECSPLGTT RTGSLLPKLD DIQKVSKYIA FAVAKVAMAQ GLALPLTDEL
     LNKSIEKNFW EPKYRRYKRT SF
//