Reviewed, UniProtKB/Swiss-Prot Q12QQ5 (CYSI_SHEDO)

Names and origin

Protein names

Recommended name:
    Sulfite reductase [NADPH] hemoprotein beta-component
      Short name=SIR-HP
      Short name=SIRHP
    EC=1.8.1.2

Gene names

Name:	cysI
Ordered Locus Names:	Sden_0933

Organism

Shewanella denitrificans (strain OS217 / ATCC BAA-1090 / DSM 15013) [Complete proteome] [HAMAP]

Taxonomic identifier

318161 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	562 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This enzyme catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate By similarity.
Catalytic activity	H(2)S + 3 NADP(+) + 3 H(2)O = sulfite + 3 NADPH.
Cofactor	Binds 1 siroheme per subunit By similarity. Binds 1 4Fe-4S cluster per subunit By similarity.
Subunit structure	Alpha(8)-beta(4). The alpha component is a flavoprotein, the beta component is a hemoprotein By similarity.
Sequence similarities	Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	4Fe-4S Heme Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation Inferred from electronic annotation. Source: HAMAP
Cellular component	sulfite reductase complex (NADPH) Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW sulfite reductase (NADPH) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 562	562	Sulfite reductase [NADPH] hemoprotein beta-component		PRO_0000292963
Sites
	Metal binding	426	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	432	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	471	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	475	1	Iron (siroheme axial ligand) By similarity
	Metal binding	475	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q12QQ5-1 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 85847875212193C6	FASTA	562	62,333
	10 20 30 40 50 60 MSEQKLAVNE YLKTDSDYLR GTIQEGLDTQ VTGSFSDGDQ QLIKFHGFYQ QDDRDLRNER 70 80 90 100 110 120 KEQKLEPLYS FMLRARVPGG ICTPAQWLDV DKISSTLTTS NSIRLTTRQT FQYHGIPKRN 130 140 150 160 170 180 LKTLIQGLDK AALDSIAACG DVNRNVMCNP NPVESKLHAQ AYAVAKELSD HLLPHTRAYA 190 200 210 220 230 240 EIWLDEEKLV GETVEPVYGN TYLPRKFKMA VSVPPDNDVD VYTNDLGFIA IAEDGQLVGF 250 260 270 280 290 300 NLVAGGGMGS THGEVETFPR LADDFGFIKT ADVIKFAEAV MTVQRDWGNR VVRKRARLKY 310 320 330 340 350 360 TIVDHGFDAF KAEVENRAGV KFAPKRDVVI GDRGDRYGWV EGIDSKWHLT LFIESGRIKD 370 380 390 400 410 420 LPGQTLQTGL REIAKIHKGD FRMTSNQNMI IAGVAAEDKA EIEGLARKHG LMGQVLTGTR 430 440 450 460 470 480 GHSIACVALP TCPLAMAEAE RYFPEFIDHI DALQAKHGIS DQSIVVRMTG CPNGCARPFA 490 500 510 520 530 540 AEIGLVGKAP GRYNLYLGAN FEGTRLNKMH KENIQEAEIL AELDTLFGRY ATERDAGETF 550 560 GNFTVRIGVV KAVNDAAKDF HG

References

[1]

"Complete sequence of Shewanella denitrificans OS217." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000302 Genomic DNA. Translation: ABE54221.1.
RefSeq	YP_561944.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4016878.
GenomeReviews	Gene locus Sden_0933 in contig CP000302_GR.
KEGG	sdn:Sden_0933.
NMPDR	fig|318161.14.peg.914.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q12QQ5.
Enzyme and pathway databases
BioCyc	SDEN318161:SDEN_0933-MON.
Family and domain databases
HAMAP	MF_01540. [Tree]
InterPro	IPR011786. CysI. IPR006066. Nir_Si_BS. IPR006067. Nir_Sir_4Fe4S. IPR005117. NiRdtase/SiRdtase_haem-b_fer. [Graphical view]
Pfam	PF01077. NIR_SIR. 1 hit. PF03460. NIR_SIR_ferr. 2 hits. [Graphical view]
PRINTS	PR00397. SIROHAEM.
TIGRFAMs	TIGR02041. CysI. 1 hit.
PROSITE	PS00365. NIR_SIR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSI_SHEDO

Accession

Primary (citable) accession number: Q12QQ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	August 22, 2006
Last modified:	November 25, 2008
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)