Q12PZ2 (ISPD_SHEDO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
EC=2.7.7.60

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase
MEP cytidylyltransferase
Short name=MCT

Gene names

Name:	ispD
Ordered Locus Names:	Sden_1198

Organism

Shewanella denitrificans (strain OS217 / ATCC BAA-1090 / DSM 15013) [Complete proteome] [HAMAP]

Taxonomic identifier

318161 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	241 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108
Sequence similarities	Belongs to the IspD family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 241	241	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_00108		PRO_1000191069
Sites
	Site	25	1	Transition state stabilizer By similarity
	Site	32	1	Transition state stabilizer By similarity
	Site	161	1	Positions MEP for the nucleophilic attack By similarity
	Site	217	1	Positions MEP for the nucleophilic attack By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q12PZ2 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 26C2C849B46B1F92	FASTA	241	25,459
	10 20 30 40 50 60 MSMTHTTPLV PKNVAIVPAA GIGARMAAGM PKQYLTLGAQ SILGHTLDAL LSHPQIAQVI 70 80 90 100 110 120 VALHPHDDMF LGLPQAKHSK LLTVIGGGER ADSVLAALAV APQDAWALVH DAARPCLTHS 130 140 150 160 170 180 DIDKLLASTS EFPQGAILAM PVRDTMKRSD DTGVINHTVD RALLWHALTP QYFPVAELLT 190 200 210 220 230 240 NLSAALNAGV VITDEASAME WAGVFPGLVS GRADNIKVTH PDDLHLAGLF MAHLSAKPLV E

References

[1]

"Complete sequence of Shewanella denitrificans OS217." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: OS217 / ATCC BAA-1090 / DSM 15013.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000302 Genomic DNA. Translation: ABE54484.1.
RefSeq	YP_562207.1. NC_007954.1.
3D structure databases
ProteinModelPortal	Q12PZ2.
ModBase	Search...
Protein-protein interaction databases
STRING	Q12PZ2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4017721.
GenomeReviews	Gene locus Sden_1198 in contig CP000302_GR.
KEGG	sdn:Sden_1198.
NMPDR	fig|318161.14.peg.1173.
PATRIC	23488158. VBISheDen79529_1277.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1211.
HOGENOM	HBG672839.
OMA	FPQGAIL.
PhylomeDB	Q12PZ2.
ProtClustDB	PRK00155.
Enzyme and pathway databases
BioCyc	SDEN318161:SDEN_1198-MONOMER.
Family and domain databases
HAMAP	MF_00108. IspD. [Tree]
InterPro	IPR001228. ISPD_synthase. [Graphical view]
KO	K00991.
Pfam	PF01128. IspD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00453. IspD. 1 hit.
PROSITE	PS01295. ISPD. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPD_SHEDO

Accession

Primary (citable) accession number: Q12PZ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	August 22, 2006
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program