Skip Header

Contribute Send feedback
Read comments (?) or add your own

Q12PR7 (PUR4_SHEDO) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoribosylformylglycinamidine synthase
Short name=FGAM synthase
Short name=FGAMS
EC=6.3.5.3
Alternative name(s):
Formylglycinamide ribotide amidotransferase
Short name=FGARAT
Formylglycinamide ribotide synthetase
Gene names
Name:purL
Ordered Locus Names:Sden_1273
OrganismShewanella denitrificans (strain OS217 / ATCC BAA-1090 / DSM 15013) [Complete proteome] [HAMAP]
Taxonomic identifier318161 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length1293 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00419

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00419

Subunit structure

Monomer.

Subcellular location

Cytoplasm HAMAP MF_00419.

Sequence similarities

In the N-terminal section; belongs to the FGAMS family.

Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
   Biological processPurine biosynthesis
   Cellular componentCytoplasm
   DomainGlutamine amidotransferase
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological process'de novo' IMP biosynthetic process

Inferred from electronic annotation. Source: InterPro

glutamine metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoribosylformylglycinamidine synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12931293Phosphoribosylformylglycinamidine synthase HAMAP MF_00419
PRO_0000264593

Regions

Domain1040 – 1293254Glutamine amidotransferase type-1
Nucleotide binding305 – 31612ATP Potential

Sites

Active site11331Nucleophile By similarity
Active site12581 By similarity
Active site12601 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q12PR7 [UniParc].

Last modified August 22, 2006. Version 1.
Checksum: A916126B88D3D748

FASTA1,293140,347
        10         20         30         40         50         60 
MEIIRGAPAL SAFRVQQLMK ACDAAAIPVR HIYAEFMHFA HLTNALNDTQ TLQLASILTY 

        70         80         90        100        110        120 
GPAIEAHTPK GQLYLVTPRP GTISPWSSKA TDIAHNCGLS QIKRLERGIA YYVEADTLDA 

       130        140        150        160        170        180 
SQQKALQALL YDRMVEVIFD DMAAAETLFD RTEPKALASV NVLGEGRRAL EVANSRLGLA 

       190        200        210        220        230        240 
LAEDEIDYLV DNFVRLKRNP NDIELMMFAQ ANSEHCRHKI FNADWTIDGV VQDKSLFKMI 

       250        260        270        280        290        300 
KNTYAVTPDN VLSAYKDNAA VMTGSVAGRF FPDETGVYAY HVEPCHILMK VETHNHPTAI 

       310        320        330        340        350        360 
SPYAGAATGS GGEIRDEGAT GRGSKPKAGL VGFSVSNLNI PGFIQPWEAQ YGKPERIVTP 

       370        380        390        400        410        420 
LEIMLEGPLG GAAFNNEFGR PALVGYFRTY EQEVSSHNGV EVRGYHKPIM LAGGLGNIRE 

       430        440        450        460        470        480 
DHVQKGEITV GAKLIVLGGP AMNIGLGGGA ASSMASGQSS EDLDFASVQR ENPEMERRCQ 

       490        500        510        520        530        540 
EVIDRCWQLG DDNPIQFIHD VGAGGLSNAF PELVNDADRG GRFNLRNVPS DEPGMSPLEI 

       550        560        570        580        590        600 
WCNESQERYV LSVAPENLAR FEQICLRERA PFAVVGEATS EQHLTLADSH FNNKPIDLPL 

       610        620        630        640        650        660 
EVLLGKAPKM SRDVVSKKAL SPALDESQIT VDEAVTRILS LPTVADKSFL ITIGDRSVTG 

       670        680        690        700        710        720 
LVNRDQMVGP WQVPVADCAV TAASFDTYAG EAMSIGERTP LALLDFGASA RMAVAESIMN 

       730        740        750        760        770        780 
IAGTDIGSFK RIKLSANWMS AAGHPGEDAG LYEAVKAVGE ELCPQLEITI PVGKDSMSMK 

       790        800        810        820        830        840 
TAWEDNGVHK SVTSPMSLVI TAFGVVQDIR NTVTPELRSD KGDTALLLLD LSHGKTRLGG 

       850        860        870        880        890        900 
SCLAQVFSSL GDVAPDLDDS TTLKGFFEVM QTLVADQSIL AYHDRSDGGL FTTVTEMAFA 

       910        920        930        940        950        960 
GNTGAEIDLS ALQGSDLARL FNEELGAVIQ VSQAQAEAIT AQFIAAGVAC HAIGGLTEHN 

       970        980        990       1000       1010       1020 
KLVVKDGSRV VFQQQRSELR RLWSQTSYKM QALRDNPDCA LEEFSLKQSE TDPGLTVKLN 

      1030       1040       1050       1060       1070       1080 
FDPSQDVAAP YILKGIAPKM AILREQGVNS HVEMAAAFDR AGFESRDVHM SDILSGRISL 

      1090       1100       1110       1120       1130       1140 
DEFQGLVACG GFSYGDVLGA GEGWAKSILF NQRARDEFSR FFERDLSFAL GVCNGCQMLS 

      1150       1160       1170       1180       1190       1200 
NLKEIIPGSE HWPRFVRNRS ERFEARVSLV EVQQSPSLFF EGMAGSRMPI AVSHGEGLAE 

      1210       1220       1230       1240       1250       1260 
FASMQAMTAA ESTGTVALRY VTGTGEIATQ YPQNPNGSPN GLSGICSTDG KVTIMMPHPE 

      1270       1280       1290 
RVFRTVANSW HPDNWGEDSP WMRMFRNARV KLG

« Hide

References

[1]"Complete sequence of Shewanella denitrificans OS217."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Richardson P.
Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: OS217 / ATCC BAA-1090 / DSM 15013.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000302 Genomic DNA. Translation: ABE54559.1.
RefSeqYP_562282.1. NC_007954.1.

3D structure databases

ProteinModelPortalQ12PR7.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ12PR7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4017541.
GenomeReviewsGene locus Sden_1273 in contig CP000302_GR.
KEGGsdn:Sden_1273.
NMPDRfig|318161.14.peg.1247.
PATRIC23488332. VBISheDen79529_1358.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0046.
HOGENOMHBG335309.
OMAVKAVGME.
PhylomeDBQ12PR7.
ProtClustDBPRK05297.

Enzyme and pathway databases

BioCycSDEN318161:SDEN_1273-MONOMER.

Family and domain databases

HAMAPMF_00419. PurL_1.
[Tree]
InterProIPR000728. AIR_synth.
IPR010918. AIR_synth_C.
IPR017926. GATASE_1.
IPR010073. PRibForGlyAmidine_synth.
IPR022940. PRibForGlyAmidine_synth_bac.
IPR016188. PurM_N-like.
[Graphical view]
KOK01952.
PfamPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 2 hits.
[Graphical view]
SUPFAMSSF56042. AIR_synth_C. 2 hits.
SSF55326. PurM_N-like. 2 hits.
TIGRFAMsTIGR01735. FGAM_synt. 1 hit.
PROSITEPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR4_SHEDO
AccessionPrimary (citable) accession number: Q12PR7
Entry history
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: August 22, 2006
Last modified: January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents