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Q12P44

- NAPA_SHEDO

UniProt

Q12P44 - NAPA_SHEDO

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Protein

Periplasmic nitrate reductase

Gene
napA, Sden_1497
Organism
Shewanella denitrificans (strain OS217 / ATCC BAA-1090 / DSM 15013)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster By similarity.UniRule annotation
Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi46 – 461Iron-sulfur (4Fe-4S) By similarity
Metal bindingi49 – 491Iron-sulfur (4Fe-4S) By similarity
Metal bindingi53 – 531Iron-sulfur (4Fe-4S) By similarity
Metal bindingi81 – 811Iron-sulfur (4Fe-4S) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciSDEN318161:GHKQ-1554-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductase (EC:1.7.99.4)
Gene namesi
Name:napA
Ordered Locus Names:Sden_1497
OrganismiShewanella denitrificans (strain OS217 / ATCC BAA-1090 / DSM 15013)
Taxonomic identifieri318161 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
ProteomesiUP000001982: Chromosome

Subcellular locationi

Periplasm By similarity UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Tat-type signal Reviewed predictionAdd
BLAST
Chaini28 – 829802Periplasmic nitrate reductaseUniRule annotationPRO_5000114527Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Interacts with NapB By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi318161.Sden_1497.

Structurei

3D structure databases

ProteinModelPortaliQ12P44.
SMRiQ12P44. Positions 39-826.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 95574Fe-4S Mo/W bis-MGD-typeAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiLVYMHPE.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12P44-1 [UniParc]FASTAAdd to Basket

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MNRRDFMKAN AVIAAASAAG LALPAGASNL ITSSEQTKLE WNKAPCRFCG    50
TGCSVMVATR EGKVVATHGD ANSEVNRGLS CIKGYFLSKI MYGRDRLTSP 100
MLRMTDGKYD KHGEFTPVSW EKAFDTMAER WKATIKEKGP TAVGMFGSGQ 150
WTVWEGYAAV KLMKAGFGTN NIDPNARHCM ASAVAGFMRT FGIDEPMGCY 200
DDMEAADAFV LWGSNMAEMH PILWTRVTDR RLSAPHVKVA VLSTFEHRSF 250
DLADLPMVFH PQTDLAILNF IANYIIQNNK VNWDFVNKHV NFRKGTTDIG 300
YGLRPAHPTQ MKSKNAATAN DSTPIDFEQF KKFVADYDVE SVSKLSGVPE 350
HKLLELAELY ADPKVKVTSF WTMGFNQHTR GVWCNNLMYN IHLLVGKIST 400
PGNSPFSLTG QPSACGTARE VGTFSHRLPA DMVVTDPKHR KIAENIWKIP 450
SGIIPEKPGY HAVEQSRRLK DGDLNCYWVQ VNNNMQAGPN INEEGLPGYR 500
NPANFIVVSD AYPTVTTQAA DLILPTAMWV EKEGAYGNAE RRTQFWHQMV 550
KAPGESKSDL WQLMEFSKRF TTDEVWSKAV LDANPKYKGK TLFEVLFKNG 600
QVDKFPLADA DPKYMNDEND AFGFYVQKGL FEEYATFGRG HGHDLADFDT 650
YHKEHGLRWP VVDGKETKWR FREGSDPYVK AGTGFEFYGK PDGRAVIFAL 700
PYEPAAEAPD EEFDMWLSTG RVLEHWHSGS MTQRVPELYK AFPDAVCFMH 750
PDDAKKRGLR RGDEVKVMSR RGEIKTRIET RGRNKPPVGL VFVPWFDASQ 800
LINKVTLDAT DPISKQTDFK KCAVKVIKA 829
Length:829
Mass (Da):93,039
Last modified:August 22, 2006 - v1
Checksum:iF452E7CAE302C02F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000302 Genomic DNA. Translation: ABE54782.1.
RefSeqiYP_562505.1. NC_007954.1.

Genome annotation databases

EnsemblBacteriaiABE54782; ABE54782; Sden_1497.
GeneIDi4017266.
KEGGisdn:Sden_1497.
PATRICi23488832. VBISheDen79529_1593.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000302 Genomic DNA. Translation: ABE54782.1 .
RefSeqi YP_562505.1. NC_007954.1.

3D structure databases

ProteinModelPortali Q12P44.
SMRi Q12P44. Positions 39-826.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 318161.Sden_1497.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABE54782 ; ABE54782 ; Sden_1497 .
GeneIDi 4017266.
KEGGi sdn:Sden_1497.
PATRICi 23488832. VBISheDen79529_1593.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OMAi LVYMHPE.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

BioCyci SDEN318161:GHKQ-1554-MONOMER.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: OS217 / ATCC BAA-1090 / DSM 15013.

Entry informationi

Entry nameiNAPA_SHEDO
AccessioniPrimary (citable) accession number: Q12P44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: August 22, 2006
Last modified: May 14, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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