Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Shewanella denitrificans (strain OS217 / ATCC BAA-1090 / DSM 15013)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite.UniRule annotation

Catalytic activityi

2 ferrocytochrome + nitrate + 2 H+ = 2 ferricytochrome + nitrite.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi46Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi49Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi53Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi81Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei83Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei150Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei175Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei179Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei373Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei377Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei483Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei532Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei559Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei795Substrate; via amide nitrogenUniRule annotation1
Binding sitei803Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei820Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Nitrate assimilation, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.9.6.1UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:Sden_1497
OrganismiShewanella denitrificans (strain OS217 / ATCC BAA-1090 / DSM 15013)
Taxonomic identifieri318161 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
Proteomesi
  • UP000001982 Componenti: Chromosome

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Tat-type signalUniRule annotationAdd BLAST27
ChainiPRO_500011452728 – 829Periplasmic nitrate reductaseUniRule annotationAdd BLAST802

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Component of the periplasmic nitrate reductase NapAB complex composed of NapA and NapB.UniRule annotation

Protein-protein interaction databases

STRINGi318161.Sden_1497.

Structurei

3D structure databases

ProteinModelPortaliQ12P44.
SMRiQ12P44.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini39 – 954Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni212 – 219Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation8
Regioni243 – 247Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation5
Regioni262 – 264Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation3
Regioni509 – 510Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation2
Regioni719 – 728Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation10

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107QIW. Bacteria.
COG0243. LUCA.
HOGENOMiHOG000031441.
KOiK02567.
OMAiTTDEVWP.
OrthoDBiPOG091H060P.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct_NapA. 1 hit.
InterProiView protein in InterPro
IPR009010. Asp_de-COase-like_dom_sf.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
PANTHERiPTHR11615:SF123. PTHR11615:SF123. 1 hit.
PfamiView protein in Pfam
PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
SMARTiView protein in SMART
SM00926. Molybdop_Fe4S4. 1 hit.
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
PROSITEiView protein in PROSITE
PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12P44-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRRDFMKAN AVIAAASAAG LALPAGASNL ITSSEQTKLE WNKAPCRFCG
60 70 80 90 100
TGCSVMVATR EGKVVATHGD ANSEVNRGLS CIKGYFLSKI MYGRDRLTSP
110 120 130 140 150
MLRMTDGKYD KHGEFTPVSW EKAFDTMAER WKATIKEKGP TAVGMFGSGQ
160 170 180 190 200
WTVWEGYAAV KLMKAGFGTN NIDPNARHCM ASAVAGFMRT FGIDEPMGCY
210 220 230 240 250
DDMEAADAFV LWGSNMAEMH PILWTRVTDR RLSAPHVKVA VLSTFEHRSF
260 270 280 290 300
DLADLPMVFH PQTDLAILNF IANYIIQNNK VNWDFVNKHV NFRKGTTDIG
310 320 330 340 350
YGLRPAHPTQ MKSKNAATAN DSTPIDFEQF KKFVADYDVE SVSKLSGVPE
360 370 380 390 400
HKLLELAELY ADPKVKVTSF WTMGFNQHTR GVWCNNLMYN IHLLVGKIST
410 420 430 440 450
PGNSPFSLTG QPSACGTARE VGTFSHRLPA DMVVTDPKHR KIAENIWKIP
460 470 480 490 500
SGIIPEKPGY HAVEQSRRLK DGDLNCYWVQ VNNNMQAGPN INEEGLPGYR
510 520 530 540 550
NPANFIVVSD AYPTVTTQAA DLILPTAMWV EKEGAYGNAE RRTQFWHQMV
560 570 580 590 600
KAPGESKSDL WQLMEFSKRF TTDEVWSKAV LDANPKYKGK TLFEVLFKNG
610 620 630 640 650
QVDKFPLADA DPKYMNDEND AFGFYVQKGL FEEYATFGRG HGHDLADFDT
660 670 680 690 700
YHKEHGLRWP VVDGKETKWR FREGSDPYVK AGTGFEFYGK PDGRAVIFAL
710 720 730 740 750
PYEPAAEAPD EEFDMWLSTG RVLEHWHSGS MTQRVPELYK AFPDAVCFMH
760 770 780 790 800
PDDAKKRGLR RGDEVKVMSR RGEIKTRIET RGRNKPPVGL VFVPWFDASQ
810 820
LINKVTLDAT DPISKQTDFK KCAVKVIKA
Length:829
Mass (Da):93,039
Last modified:August 22, 2006 - v1
Checksum:iF452E7CAE302C02F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000302 Genomic DNA. Translation: ABE54782.1.
RefSeqiWP_011495940.1. NC_007954.1.

Genome annotation databases

EnsemblBacteriaiABE54782; ABE54782; Sden_1497.
KEGGisdn:Sden_1497.

Similar proteinsi

Entry informationi

Entry nameiNAPA_SHEDO
AccessioniPrimary (citable) accession number: Q12P44
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: August 22, 2006
Last modified: January 31, 2018
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families