Reviewed, UniProtKB/Swiss-Prot Q12P44 (NAPA_SHEDO)

Names and origin

Protein names

Recommended name:
    Periplasmic nitrate reductase
    EC=1.7.99.4

Gene names

Name:	napA
Ordered Locus Names:	Sden_1497

Organism

Shewanella denitrificans (strain OS217 / ATCC BAA-1090 / DSM 15013) [Complete proteome] [HAMAP]

Taxonomic identifier

318161 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	829 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP MF_01630
Catalytic activity	Nitrite + acceptor = nitrate + reduced acceptor. HAMAP MF_01630
Cofactor	Binds 1 4Fe-4S cluster By similarity. HAMAP MF_01630 Binds 1 molybdenum ion per subunit By similarity. HAMAP MF_01630 Binds 2 molybdopterin guanine dinucleotide (MGD) groups per subunit By similarity. HAMAP MF_01630
Subunit structure	Interacts with napB By similarity. HAMAP MF_01630
Subcellular location	Periplasm By similarity HAMAP MF_01630.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP MF_01630
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily.

Ontologies

Keywords
Biological process	Electron transport Nitrate assimilation Transport
Cellular component	Periplasm
Domain	Signal
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Molybdenum
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	Mo-molybdopterin cofactor biosynthetic process Inferred from electronic annotation. Source: HAMAP electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW nitrate assimilation Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP molybdenum ion binding Inferred from electronic annotation. Source: UniProtKB-KW nitrate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Tat-type signal Potential
	Chain	28 – 829	802	Periplasmic nitrate reductase HAMAP MF_01630		PRO_5000114527
Sites
	Metal binding	46	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	49	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	53	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	81	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q12P44-1 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: F452E7CAE302C02F	FASTA	829	93,039
	10 20 30 40 50 60 MNRRDFMKAN AVIAAASAAG LALPAGASNL ITSSEQTKLE WNKAPCRFCG TGCSVMVATR 70 80 90 100 110 120 EGKVVATHGD ANSEVNRGLS CIKGYFLSKI MYGRDRLTSP MLRMTDGKYD KHGEFTPVSW 130 140 150 160 170 180 EKAFDTMAER WKATIKEKGP TAVGMFGSGQ WTVWEGYAAV KLMKAGFGTN NIDPNARHCM 190 200 210 220 230 240 ASAVAGFMRT FGIDEPMGCY DDMEAADAFV LWGSNMAEMH PILWTRVTDR RLSAPHVKVA 250 260 270 280 290 300 VLSTFEHRSF DLADLPMVFH PQTDLAILNF IANYIIQNNK VNWDFVNKHV NFRKGTTDIG 310 320 330 340 350 360 YGLRPAHPTQ MKSKNAATAN DSTPIDFEQF KKFVADYDVE SVSKLSGVPE HKLLELAELY 370 380 390 400 410 420 ADPKVKVTSF WTMGFNQHTR GVWCNNLMYN IHLLVGKIST PGNSPFSLTG QPSACGTARE 430 440 450 460 470 480 VGTFSHRLPA DMVVTDPKHR KIAENIWKIP SGIIPEKPGY HAVEQSRRLK DGDLNCYWVQ 490 500 510 520 530 540 VNNNMQAGPN INEEGLPGYR NPANFIVVSD AYPTVTTQAA DLILPTAMWV EKEGAYGNAE 550 560 570 580 590 600 RRTQFWHQMV KAPGESKSDL WQLMEFSKRF TTDEVWSKAV LDANPKYKGK TLFEVLFKNG 610 620 630 640 650 660 QVDKFPLADA DPKYMNDEND AFGFYVQKGL FEEYATFGRG HGHDLADFDT YHKEHGLRWP 670 680 690 700 710 720 VVDGKETKWR FREGSDPYVK AGTGFEFYGK PDGRAVIFAL PYEPAAEAPD EEFDMWLSTG 730 740 750 760 770 780 RVLEHWHSGS MTQRVPELYK AFPDAVCFMH PDDAKKRGLR RGDEVKVMSR RGEIKTRIET 790 800 810 820 RGRNKPPVGL VFVPWFDASQ LINKVTLDAT DPISKQTDFK KCAVKVIKA

References

[1]

"Complete sequence of Shewanella denitrificans OS217." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000302 Genomic DNA. Translation: ABE54782.1.
RefSeq	YP_562505.1.
3D structure databases
SMR	Q12P44. Positions 39-826.
ModBase	Search...
Protein-protein interaction databases
STRING	Q12P44.
Genome annotation databases
GeneID	4017266.
GenomeReviews	Gene locus Sden_1497 in contig CP000302_GR.
KEGG	sdn:Sden_1497.
NMPDR	fig|318161.14.peg.1462.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0243.
HOGENOM	HBG715033.
OMA	QFWHQLV.
PhylomeDB	Q12P44.
Family and domain databases
HAMAP	MF_01630. Nitrate_reduct. [Tree]
InterPro	IPR009010. Asp_de-COase-like_fold. IPR006657. MoPterin_dinucl-bd_dom. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4_dom. IPR006655. Mopterin_OxRdtase_prok_CS. IPR010051. NO3_reductase_lsu_periplasm. IPR006311. TAT_signal. IPR017909. Twin_arg_translocation_Tat. [Graphical view]
Gene3D	G3DSA:2.40.40.20. Asp_decarboxylase-like_fold. 1 hit.
Pfam	PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR01706. NAPA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. 1 hit. PS00490. MOLYBDOPTERIN_PROK_2. False negative. PS00932. MOLYBDOPTERIN_PROK_3. False negative. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAPA_SHEDO

Accession

Primary (citable) accession number: Q12P44

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	August 22, 2006
Last modified:	February 9, 2010
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)