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Q12P44 (NAPA_SHEDO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:Sden_1497
OrganismShewanella denitrificans (strain OS217 / ATCC BAA-1090 / DSM 15013) [Complete proteome] [HAMAP]
Taxonomic identifier318161 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella

Protein attributes

Sequence length829 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727Tat-type signal Potential
Chain28 – 829802Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_5000114527

Regions

Domain39 – 95574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding461Iron-sulfur (4Fe-4S) By similarity
Metal binding491Iron-sulfur (4Fe-4S) By similarity
Metal binding531Iron-sulfur (4Fe-4S) By similarity
Metal binding811Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q12P44 [UniParc].

Last modified August 22, 2006. Version 1.
Checksum: F452E7CAE302C02F

FASTA82993,039
        10         20         30         40         50         60 
MNRRDFMKAN AVIAAASAAG LALPAGASNL ITSSEQTKLE WNKAPCRFCG TGCSVMVATR 

        70         80         90        100        110        120 
EGKVVATHGD ANSEVNRGLS CIKGYFLSKI MYGRDRLTSP MLRMTDGKYD KHGEFTPVSW 

       130        140        150        160        170        180 
EKAFDTMAER WKATIKEKGP TAVGMFGSGQ WTVWEGYAAV KLMKAGFGTN NIDPNARHCM 

       190        200        210        220        230        240 
ASAVAGFMRT FGIDEPMGCY DDMEAADAFV LWGSNMAEMH PILWTRVTDR RLSAPHVKVA 

       250        260        270        280        290        300 
VLSTFEHRSF DLADLPMVFH PQTDLAILNF IANYIIQNNK VNWDFVNKHV NFRKGTTDIG 

       310        320        330        340        350        360 
YGLRPAHPTQ MKSKNAATAN DSTPIDFEQF KKFVADYDVE SVSKLSGVPE HKLLELAELY 

       370        380        390        400        410        420 
ADPKVKVTSF WTMGFNQHTR GVWCNNLMYN IHLLVGKIST PGNSPFSLTG QPSACGTARE 

       430        440        450        460        470        480 
VGTFSHRLPA DMVVTDPKHR KIAENIWKIP SGIIPEKPGY HAVEQSRRLK DGDLNCYWVQ 

       490        500        510        520        530        540 
VNNNMQAGPN INEEGLPGYR NPANFIVVSD AYPTVTTQAA DLILPTAMWV EKEGAYGNAE 

       550        560        570        580        590        600 
RRTQFWHQMV KAPGESKSDL WQLMEFSKRF TTDEVWSKAV LDANPKYKGK TLFEVLFKNG 

       610        620        630        640        650        660 
QVDKFPLADA DPKYMNDEND AFGFYVQKGL FEEYATFGRG HGHDLADFDT YHKEHGLRWP 

       670        680        690        700        710        720 
VVDGKETKWR FREGSDPYVK AGTGFEFYGK PDGRAVIFAL PYEPAAEAPD EEFDMWLSTG 

       730        740        750        760        770        780 
RVLEHWHSGS MTQRVPELYK AFPDAVCFMH PDDAKKRGLR RGDEVKVMSR RGEIKTRIET 

       790        800        810        820 
RGRNKPPVGL VFVPWFDASQ LINKVTLDAT DPISKQTDFK KCAVKVIKA 

« Hide

References

[1]"Complete sequence of Shewanella denitrificans OS217."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Richardson P.
Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: OS217 / ATCC BAA-1090 / DSM 15013.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000302 Genomic DNA. Translation: ABE54782.1.
RefSeqYP_562505.1. NC_007954.1.

3D structure databases

ProteinModelPortalQ12P44.
SMRQ12P44. Positions 39-826.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING318161.Sden_1497.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABE54782; ABE54782; Sden_1497.
GeneID4017266.
KEGGsdn:Sden_1497.
PATRIC23488832. VBISheDen79529_1593.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMALVYMHPE.
OrthoDBEOG6CVV7G.

Enzyme and pathway databases

BioCycSDEN318161:GHKQ-1554-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_SHEDO
AccessionPrimary (citable) accession number: Q12P44
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: August 22, 2006
Last modified: May 14, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families