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Q12P44

- NAPA_SHEDO

UniProt

Q12P44 - NAPA_SHEDO

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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Shewanella denitrificans (strain OS217 / ATCC BAA-1090 / DSM 15013)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi46 – 461Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi49 – 491Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi53 – 531Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi81 – 811Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciSDEN318161:GHKQ-1554-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:Sden_1497
OrganismiShewanella denitrificans (strain OS217 / ATCC BAA-1090 / DSM 15013)
Taxonomic identifieri318161 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
ProteomesiUP000001982: Chromosome

Subcellular locationi

Periplasm UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Tat-type signalUniRule annotationAdd
BLAST
Chaini28 – 829802Periplasmic nitrate reductasePRO_5000114527Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Interactioni

Subunit structurei

Interacts with NapB.UniRule annotation

Protein-protein interaction databases

STRINGi318161.Sden_1497.

Structurei

3D structure databases

ProteinModelPortaliQ12P44.
SMRiQ12P44. Positions 39-826.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 95574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiLVYMHPE.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12P44-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNRRDFMKAN AVIAAASAAG LALPAGASNL ITSSEQTKLE WNKAPCRFCG
60 70 80 90 100
TGCSVMVATR EGKVVATHGD ANSEVNRGLS CIKGYFLSKI MYGRDRLTSP
110 120 130 140 150
MLRMTDGKYD KHGEFTPVSW EKAFDTMAER WKATIKEKGP TAVGMFGSGQ
160 170 180 190 200
WTVWEGYAAV KLMKAGFGTN NIDPNARHCM ASAVAGFMRT FGIDEPMGCY
210 220 230 240 250
DDMEAADAFV LWGSNMAEMH PILWTRVTDR RLSAPHVKVA VLSTFEHRSF
260 270 280 290 300
DLADLPMVFH PQTDLAILNF IANYIIQNNK VNWDFVNKHV NFRKGTTDIG
310 320 330 340 350
YGLRPAHPTQ MKSKNAATAN DSTPIDFEQF KKFVADYDVE SVSKLSGVPE
360 370 380 390 400
HKLLELAELY ADPKVKVTSF WTMGFNQHTR GVWCNNLMYN IHLLVGKIST
410 420 430 440 450
PGNSPFSLTG QPSACGTARE VGTFSHRLPA DMVVTDPKHR KIAENIWKIP
460 470 480 490 500
SGIIPEKPGY HAVEQSRRLK DGDLNCYWVQ VNNNMQAGPN INEEGLPGYR
510 520 530 540 550
NPANFIVVSD AYPTVTTQAA DLILPTAMWV EKEGAYGNAE RRTQFWHQMV
560 570 580 590 600
KAPGESKSDL WQLMEFSKRF TTDEVWSKAV LDANPKYKGK TLFEVLFKNG
610 620 630 640 650
QVDKFPLADA DPKYMNDEND AFGFYVQKGL FEEYATFGRG HGHDLADFDT
660 670 680 690 700
YHKEHGLRWP VVDGKETKWR FREGSDPYVK AGTGFEFYGK PDGRAVIFAL
710 720 730 740 750
PYEPAAEAPD EEFDMWLSTG RVLEHWHSGS MTQRVPELYK AFPDAVCFMH
760 770 780 790 800
PDDAKKRGLR RGDEVKVMSR RGEIKTRIET RGRNKPPVGL VFVPWFDASQ
810 820
LINKVTLDAT DPISKQTDFK KCAVKVIKA
Length:829
Mass (Da):93,039
Last modified:August 22, 2006 - v1
Checksum:iF452E7CAE302C02F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000302 Genomic DNA. Translation: ABE54782.1.
RefSeqiWP_011495940.1. NC_007954.1.
YP_562505.1. NC_007954.1.

Genome annotation databases

EnsemblBacteriaiABE54782; ABE54782; Sden_1497.
GeneIDi4017266.
KEGGisdn:Sden_1497.
PATRICi23488832. VBISheDen79529_1593.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000302 Genomic DNA. Translation: ABE54782.1 .
RefSeqi WP_011495940.1. NC_007954.1.
YP_562505.1. NC_007954.1.

3D structure databases

ProteinModelPortali Q12P44.
SMRi Q12P44. Positions 39-826.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 318161.Sden_1497.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABE54782 ; ABE54782 ; Sden_1497 .
GeneIDi 4017266.
KEGGi sdn:Sden_1497.
PATRICi 23488832. VBISheDen79529_1593.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OMAi LVYMHPE.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

BioCyci SDEN318161:GHKQ-1554-MONOMER.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: OS217 / ATCC BAA-1090 / DSM 15013.

Entry informationi

Entry nameiNAPA_SHEDO
AccessioniPrimary (citable) accession number: Q12P44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: August 22, 2006
Last modified: November 26, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3