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Q12NN4 (BIOB_SHEDO) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:Sden_1658
OrganismShewanella denitrificans (strain OS217 / ATCC BAA-1090 / DSM 15013) [Complete proteome] [HAMAP]
Taxonomic identifier318161 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella

Protein attributes

Sequence length357 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 357357Biotin synthase HAMAP-Rule MF_01694
PRO_0000381617

Sites

Metal binding561Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding601Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding631Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1001Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1311Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1911Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2631Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q12NN4 [UniParc].

Last modified August 22, 2006. Version 1.
Checksum: EEBAB569E812819A

FASTA35739,489
        10         20         30         40         50         60 
MSLFEVRHDW KKAEIEALFA LPMNDLLFKA HTIHRESFDP NEVQISRLLS IKTGACPEDC 

        70         80         90        100        110        120 
KYCPQSARYD TGLEKERLLE IEKVLTEAKS AKAAGASRFC MGAAWRNPRD RDMPYLTQMV 

       130        140        150        160        170        180 
KDVKALGLET CMTLGMLSTE QSQKLAGAGL DYYNHNLDTS PEYYGDVITT RTYQSRLDTL 

       190        200        210        220        230        240 
SNVRASGMKV CSGGIVGMGE KASDRAGLLQ QLANLEQHPD SVPINMLVKV AGTPFEKIDD 

       250        260        270        280        290        300 
LDPLEFVRTI AVARIIMPKS RVRLSAGREK MSDELQSMCF FAGANSIFYG CKLLTTANPE 

       310        320        330        340        350 
ENDDMSLFKR LGLRPEQGPA APVAQVATNL DQEQALIAKA SALNEKATQQ FYDAGAL 

« Hide

References

[1]"Complete sequence of Shewanella denitrificans OS217."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Richardson P.
Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: OS217 / ATCC BAA-1090 / DSM 15013.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000302 Genomic DNA. Translation: ABE54942.1.
RefSeqYP_562665.1. NC_007954.1.

3D structure databases

ProteinModelPortalQ12NN4.
SMRQ12NN4. Positions 7-317.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING318161.Sden_1658.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABE54942; ABE54942; Sden_1658.
GeneID4018134.
KEGGsdn:Sden_1658.
PATRIC23489176. VBISheDen79529_1763.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OMAADRFCMG.
OrthoDBEOG622PMP.
ProtClustDBCLSK2304484.

Enzyme and pathway databases

BioCycSDEN318161:GHKQ-1718-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_SHEDO
AccessionPrimary (citable) accession number: Q12NN4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: August 22, 2006
Last modified: February 19, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways