Reviewed, UniProtKB/Swiss-Prot Q12NA7 (ASTE_SHEDO)

Names and origin

Protein names

Recommended name:
    Succinylglutamate desuccinylase
    EC=3.5.1.96

Gene names

Name:	astE
Ordered Locus Names:	Sden_1785

Organism

Shewanella denitrificans (strain OS217 / ATCC BAA-1090 / DSM 15013) [Complete proteome] [HAMAP]

Taxonomic identifier

318161 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	348 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Transforms N(2)-succinylglutamate into succinate and glutamate. HAMAP MF_00767
Catalytic activity	N-succinyl-L-glutamate + H2O = succinate + L-glutamate. HAMAP MF_00767
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 5/5. HAMAP MF_00767
Sequence similarities	Belongs to the aspA/astE family. Succinylglutamate desuccinylase subfamily.

Ontologies

Keywords
Biological process	Arginine metabolism
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine catabolic process to glutamate Inferred from electronic annotation. Source: HAMAP
Molecular function	hydrolase activity, acting on ester bonds Inferred from electronic annotation. Source: HAMAP succinylglutamate desuccinylase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 348	348	Succinylglutamate desuccinylase HAMAP MF_00767		PRO_0000262080
Sites
	Active site	228	1	Potential
	Metal binding	67	1	Zinc By similarity
	Metal binding	70	1	Zinc By similarity
	Metal binding	164	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q12NA7-1 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 3B18928CD9F64240	FASTA	348	39,197
	10 20 30 40 50 60 MQHELMDNHD FLAFTLAHPH GIAQRFSFEL GQHTQVEVWD TGVIAFLPKE KYTAQAPKQV 70 80 90 100 110 120 ILSCAVHGNE TAPIEICNRL ITELLTEKVQ AKHRTLFLIG NPPAIHNQTR FIEENMNRLF 130 140 150 160 170 180 SGAHSKGEGL THAERIRAKA LEDYVAEFYL GAPSNEQRIH YDLHTAIRPS KHEKFAIYPY 190 200 210 220 230 240 RPGRAYSGEQ IMFLAACGVD TILFHHEPTT TFSYYSSEQF NADAFTVELG KVMPFGQNDM 250 260 270 280 290 300 SRFEQTQTML TKLVSGEALG LSAFSAEKVN LYKVCRSINK RFEDFAFNFA DQAENFSAFT 310 320 330 340 KGEVLATEGG EQVLVEQPQE AIVFPNAKVP VGQRTVLCLV PAPNENIR

References

[1]

"Complete sequence of Shewanella denitrificans OS217." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000302 Genomic DNA. Translation: ABE55069.1.
RefSeq	YP_562792.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4018264.
GenomeReviews	Gene locus Sden_1785 in contig CP000302_GR.
KEGG	sdn:Sden_1785.
NMPDR	fig|318161.14.peg.1750.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q12NA7.
OMA	Q12NA7. EKFAIYP.
Enzyme and pathway databases
BioCyc	SDEN318161:SDEN_1785-MON.
Family and domain databases
HAMAP	MF_00767. [Tree]
InterPro	IPR007036. Aste_AspA. IPR016681. SuccinylGlu_desuccinylase. [Graphical view]
Pfam	PF04952. AstE_AspA. 1 hit. [Graphical view]
PIRSF	PIRSF017020. AstE. 1 hit.
TIGRFAMs	TIGR03242. arg_catab_astE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ASTE_SHEDO

Accession

Primary (citable) accession number: Q12NA7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	August 22, 2006
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)