Reviewed, UniProtKB/Swiss-Prot Q12LW6 (SPEA_SHEDO)

Names and origin

Protein names

Recommended name:
    Biosynthetic arginine decarboxylase
      Short name=ADC
    EC=4.1.1.19

Gene names

Name:	speA
Ordered Locus Names:	Sden_2280

Organism

Shewanella denitrificans (strain OS217 / ATCC BAA-1090 / DSM 15013) [Complete proteome] [HAMAP]

Taxonomic identifier

318161 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	636 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the biosynthesis of agmatine from arginine By similarity.
Catalytic activity	L-arginine = agmatine + CO2. HAMAP MF_01417
Cofactor	Magnesium By similarity. Pyridoxal phosphate By similarity.
Pathway	Amino-acid degradation; L-arginine degradation via ADC pathway; agmatine from L-arginine: step 1/1. HAMAP MF_01417
Sequence similarities	Belongs to the Orn/Lys/Arg decarboxylase class-II family. SpeA subfamily.

Ontologies

Keywords
Biological process	Polyamine biosynthesis Spermidine biosynthesis
Ligand	Magnesium Metal-binding Pyridoxal phosphate
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine catabolic process Inferred from electronic annotation. Source: InterPro spermidine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	arginine decarboxylase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 636	636	Biosynthetic arginine decarboxylase HAMAP MF_01417		PRO_1000024267
Regions
	Region	286 – 296	11	Substrate-binding Potential
Amino acid modifications
	Modified residue	101	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q12LW6-1 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 67F1E19B36DD55CA	FASTA	636	71,065
	10 20 30 40 50 60 MNDWTIEDAR AGYNVTHWSQ GFYGIGEAGE VTVSPDPLNP SNKVALDTLA QDLVQAGIAL 70 80 90 100 110 120 PVLVRFPQIL HHRVESLCDA FNQAIQKYEY QNDYLLVYPI KVNQQKTVVE EILASQVSKE 130 140 150 160 170 180 VPQLGLEAGS KPELMAVLAM AQKASSVIVC NGYKDKEYVR LALIGEKLGH KVYIVLEKMS 190 200 210 220 230 240 ELKMVLEEAR ELGVTPRLGL RTRLAFQGKG KWQASGGEKS KFGLSAAQVL KVVDELKEAN 250 260 270 280 290 300 MLESLQLLHF HLGSQIANIR DIRQGVSEAG RFYCELRQLG ASINCFDVGG GLAVDYDGTR 310 320 330 340 350 360 SQSNNSMNYG LTEYANNIVN VLTDLCNEYE QPMPRIISES GRHLTAHHAV LITDVIGTEA 370 380 390 400 410 420 YMPENIQAPA EDAPQLLHNM WQSWTEISGR HDQRAIIEIY HDSQSDIAEA HSLFAVGQLS 430 440 450 460 470 480 LMDRAWAEQT NLRVCHEVKG LLSNNNRYHR PVIDELNEKL ADKLFVNFSL FQSLPDAWGI 490 500 510 520 530 540 DQVFPVLPLT CLDKAPERRA VMLDITCDSD GIVDQYVDGQ GIETTLPVPA WDPANPYLIG 550 560 570 580 590 600 FFMVGAYQEI LGDMHNLFGD TNSAVVFVED NGKARIESTL DGDTVADVLR YVNLDADEFM 610 620 630 HTYEELVEQH IVEDERASIL EELQLGLKGY TYLEDF

References

[1]

"Complete sequence of Shewanella denitrificans OS217." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000302 Genomic DNA. Translation: ABE55560.1.
RefSeq	YP_563283.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4018772.
GenomeReviews	Gene locus Sden_2280 in contig CP000302_GR.
KEGG	sdn:Sden_2280.
NMPDR	fig|318161.14.peg.2221.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q12LW6.
OMA	Q12LW6. LICNGYK.
Enzyme and pathway databases
BioCyc	SDEN318161:SDEN_2280-MON.
Family and domain databases
HAMAP	MF_01417. [Tree]
InterPro	IPR002985. Arg_decrbxlase. IPR000183. De-COase2. [Graphical view]
PANTHER	PTHR11482:SF3. Arg_decrbxlase. 1 hit.
Pfam	PF02784. Orn_Arg_deC_N. 1 hit. PF00278. Orn_DAP_Arg_deC. 1 hit. [Graphical view]
PIRSF	PIRSF001336. Arg_decrbxlase. 1 hit.
PRINTS	PR01180. ARGDCRBXLASE. PR01179. ODADCRBXLASE.
TIGRFAMs	TIGR01273. speA. 1 hit.
PROSITE	PS00878. ODR_DC_2_1. False negative. PS00879. ODR_DC_2_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPEA_SHEDO

Accession

Primary (citable) accession number: Q12LW6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 22, 2006
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)