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Q12LP2 (DDL_SHEDO) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:Sden_2354
OrganismShewanella denitrificans (strain OS217 / ATCC BAA-1090 / DSM 15013) [Complete proteome] [HAMAP]
Taxonomic identifier318161 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella

Protein attributes

Sequence length335 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 335335D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000074786

Regions

Domain124 – 330207ATP-grasp
Nucleotide binding154 – 20956ATP By similarity

Sites

Metal binding2841Magnesium or manganese 1 By similarity
Metal binding2971Magnesium or manganese 1 By similarity
Metal binding2971Magnesium or manganese 2 By similarity
Metal binding2991Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q12LP2 [UniParc].

Last modified August 22, 2006. Version 1.
Checksum: 112BC8E7C0123B8D

FASTA33537,585
        10         20         30         40         50         60 
MAKINVLLLC GGGGAEHDIS LMSARFFETS LAKSDQFSVL TLTLDKQGRY HTQDGNLCSL 

        70         80         90        100        110        120 
TNRREIRFED PSIAPWAVDY VIPCIHGFPG ETGDIQSYFN LIQLPYFGCE SEASSNCFNK 

       130        140        150        160        170        180 
ITAKMWFSAL GVPNTPYIFL HQFDQEAIAQ TQAAFDTWGS VFIKAASQGS SVGCYKVDVR 

       190        200        210        220        230        240 
DNIAKVLEEA FGYAPYVVVE KTIKARELEV AVYEYQGDII ATLPGEIICD TNKFYSFDEK 

       250        260        270        280        290        300 
YAKDSKARTD VVASNLSPKL CLQIQDYAIK AFKGMKLRHL SRIDFFLTQD DEILLNEINT 

       310        320        330 
FPGLTPISMF PKMLQNHGHD FTEYLSDVIS QQLGR 

« Hide

References

[1]"Complete sequence of Shewanella denitrificans OS217."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Richardson P.
Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: OS217 / ATCC BAA-1090 / DSM 15013.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000302 Genomic DNA. Translation: ABE55634.1.
RefSeqYP_563357.1. NC_007954.1.

3D structure databases

ProteinModelPortalQ12LP2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING318161.Sden_2354.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABE55634; ABE55634; Sden_2354.
GeneID4018848.
KEGGsdn:Sden_2354.
PATRIC23490633. VBISheDen79529_2475.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMAMDKIAMK.
OrthoDBEOG6ND0KB.
ProtClustDBPRK01966.

Enzyme and pathway databases

BioCycSDEN318161:GHKQ-2432-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_SHEDO
AccessionPrimary (citable) accession number: Q12LP2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: August 22, 2006
Last modified: February 19, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways