Q12LA1 (CLPP_SHEDO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent Clp protease proteolytic subunit
EC=3.4.21.92

Alternative name(s):
Endopeptidase Clp

Gene names

Name:	clpP
Ordered Locus Names:	Sden_2495

Organism

Shewanella denitrificans (strain OS217 / ATCC BAA-1090 / DSM 15013) [Complete proteome] [HAMAP]

Taxonomic identifier

318161 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	203 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins By similarity. HAMAP MF_00444
Catalytic activity	Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs). HAMAP MF_00444
Subcellular location	Cytoplasm By similarity HAMAP MF_00444.
Sequence similarities	Belongs to the peptidase S14 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 203	203	ATP-dependent Clp protease proteolytic subunit HAMAP MF_00444		PRO_1000072344
Sites
	Active site	107	1	By similarity
	Active site	132	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q12LA1 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 5A3CDA98BCC202B0	FASTA	203	22,208
	10 20 30 40 50 60 MHKAPESVLN ALVPMVVEQT AKGERSYDIY SRLLKERVIF LVGQVEEHMA NLIVAQLLFL 70 80 90 100 110 120 ESESPDKDIY LYINSPGGSV TAGMAIYDTM QFIKPNVSTV CIGQAASMGA FLLAGGAKGK 130 140 150 160 170 180 RHCLPNSRVM IHQPLGGFQG QASDIAIHAQ EILGIKNKLN QMLAEHTGQP LEVIERDTDR 190 200 DNFMSATQAM DYGLVDSLLT SRS

References

[1]

"Complete sequence of Shewanella denitrificans OS217." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: OS217 / ATCC BAA-1090 / DSM 15013.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000302 Genomic DNA. Translation: ABE55775.1.
RefSeq	YP_563498.1. NC_007954.1.
3D structure databases
ProteinModelPortal	Q12LA1.
SMR	Q12LA1. Positions 11-202.
ModBase	Search...
Protein-protein interaction databases
STRING	Q12LA1.
Protein family/group databases
MEROPS	S14.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4018995.
GenomeReviews	Gene locus Sden_2495 in contig CP000302_GR.
KEGG	sdn:Sden_2495.
NMPDR	fig|318161.14.peg.2422.
PATRIC	23490943. VBISheDen79529_2625.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0740.
HOGENOM	HBG558421.
OMA	MEAQDFG.
PhylomeDB	Q12LA1.
ProtClustDB	PRK00277.
Enzyme and pathway databases
BioCyc	SDEN318161:SDEN_2495-MONOMER.
Family and domain databases
HAMAP	MF_00444. ClpP. [Tree]
InterPro	IPR023562. Pept_S14/S49. IPR001907. Pept_S14_ClpP. IPR018215. Pept_S14_ClpP_AS. [Graphical view]
KO	K01358.
PANTHER	PTHR10381. Pept_S14_ClpP. 1 hit.
Pfam	PF00574. CLP_protease. 1 hit. [Graphical view]
PRINTS	PR00127. CLPPROTEASEP.
TIGRFAMs	TIGR00493. ClpP. 1 hit.
PROSITE	PS00382. CLP_PROTEASE_HIS. 1 hit. PS00381. CLP_PROTEASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CLPP_SHEDO

Accession

Primary (citable) accession number: Q12LA1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	August 22, 2006
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program