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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Shewanella denitrificans (strain OS217 / ATCC BAA-1090 / DSM 15013)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei243ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSDEN318161:G1G6C-2673-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:Sden_2533
OrganismiShewanella denitrificans (strain OS217 / ATCC BAA-1090 / DSM 15013)
Taxonomic identifieri318161 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
Proteomesi
  • UP000001982 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003309971 – 473Glutamate--tRNA ligaseAdd BLAST473

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi318161.Sden_2533

Structurei

3D structure databases

ProteinModelPortaliQ12L63
SMRiQ12L63
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi13 – 23"HIGH" regionUniRule annotationAdd BLAST11
Motifi240 – 244"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20 Bacteria
COG0008 LUCA
HOGENOMiHOG000252722
KOiK01885
OMAiAYYAFDT
OrthoDBiPOG091H021W

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR001412 aa-tRNA-synth_I_CS
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q12L63-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTPAITKTR FAPSPTGFLH VGGARTALYS WLQARANNGE FVLRIEDTDI
60 70 80 90 100
ERSTQAACDA ILDGMNWLGL TWDEGPYYQT KRFDRYHEII GQMLEKGTAY
110 120 130 140 150
KCYCSRERIE TMREEQAAAG LQQKYDGHCR NLAARETNEP FVIRFKNPLE
160 170 180 190 200
GSVVFDDHVR GRIEISNEML DDLIIQRTDG VPTYNFCVVV DDWDMGITSV
210 220 230 240 250
VRGEDHINNT PRQINILKAL GAPIPEYAHV SMILGDDGAK LSKRHGAVGV
260 270 280 290 300
MQYRDDGYLP EALLNYLVRL GWSHGDQEIF SMEEMKQHFK LGDINKAASA
310 320 330 340 350
FNTDKLIWLN QHYIKTLDPE YVASHLAWHM EDQNIDTSNG PALAEIVTAL
360 370 380 390 400
SERAKTLKEL AESSRYFFED FTDFDEVQAK KHLRGVALEP LTLVKTKLAA
410 420 430 440 450
LTEWSVSVIH QVIEDTATEL EVGMGKVGMP LRVAVTGAGQ SPALDLTLFL
460 470
IGKDRSEQRI SKAIDFVADR INS
Length:473
Mass (Da):53,234
Last modified:August 22, 2006 - v1
Checksum:i57EBF971371BF03E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000302 Genomic DNA Translation: ABE55813.1
RefSeqiWP_011496964.1, NC_007954.1

Genome annotation databases

EnsemblBacteriaiABE55813; ABE55813; Sden_2533
KEGGisdn:Sden_2533

Similar proteinsi

Entry informationi

Entry nameiSYE_SHEDO
AccessioniPrimary (citable) accession number: Q12L63
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: August 22, 2006
Last modified: February 28, 2018
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health