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Q12KQ0 (TRMB_SHEDO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:Sden_2697
OrganismShewanella denitrificans (strain OS217 / ATCC BAA-1090 / DSM 15013) [Complete proteome] [HAMAP]
Taxonomic identifier318161 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella ›

Protein attributes

Sequence length238 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processRNA (guanine-N7)-methylation

Inferred from electronic annotation. Source: GOC

   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 238238tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000288222

Regions

Region216 – 2194Substrate binding Potential

Sites

Binding site681S-adenosyl-L-methionine By similarity
Binding site931S-adenosyl-L-methionine By similarity
Binding site1201S-adenosyl-L-methionine By similarity
Binding site1431S-adenosyl-L-methionine By similarity
Binding site1471Substrate By similarity
Binding site1791Substrate Potential

Sequences

Sequence LengthMass (Da)Tools
Q12KQ0 [UniParc].

Last modified August 22, 2006. Version 1.
Checksum: E5C00435A5B4B256

FASTA23826,868
        10         20         30         40         50         60 
MSDVTTAEFN EEGKYLRKIR SFVLREGRLT KGQAQAIEAH WPTMGLDYTP EAIDLVKIFG 

        70         80         90        100        110        120 
READTVLEIG FGMGGSLVEM AAAAPELNYI GIEVHKPGVG ACLGVAGEAK VTNLRVYHHD 

       130        140        150        160        170        180 
AMEVLEHSIA DGSLSRVQLF FPDPWHKKRH HKRRIVQAEF AELIRRKLKL GGVFHMATDW 

       190        200        210        220        230 
ENYSEHMLEV MNLAPGYKNQ SPTGTVVERP DHRPLTKFEA RGHRLGHGVW DIMFERIS

« Hide

References

[1]"Complete sequence of Shewanella denitrificans OS217."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Richardson P.
Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: OS217 / ATCC BAA-1090 / DSM 15013.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000302 Genomic DNA. Translation: ABE55976.1.
RefSeqYP_563699.1. NC_007954.1.

3D structure databases

ProteinModelPortalQ12KQ0.
ModBaseSearch...

Protein-protein interaction databases

STRING318161.Sden_2697.

Proteomic databases

PRIDEQ12KQ0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABE55976; ABE55976; Sden_2697.
GeneID4019214.
KEGGsdn:Sden_2697.
PATRIC23491402. VBISheDen79529_2837.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OMACEVHEPG.
ProtClustDBPRK00121.

Enzyme and pathway databases

BioCycSDEN318161:GHKQ-2824-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTRMB_SHEDO
AccessionPrimary (citable) accession number: Q12KQ0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: August 22, 2006
Last modified: May 1, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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