Q12KG8 (TYSY_SHEDO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidylate synthase
Short name=TS
Short name=TSase
EC=2.1.1.45

Gene names

Name:	thyA
Ordered Locus Names:	Sden_2779

Organism

Shewanella denitrificans (strain OS217 / ATCC BAA-1090 / DSM 15013) [Complete proteome] [HAMAP]

Taxonomic identifier

318161 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP MF_00008
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008
Subunit structure	Homodimer By similarity. HAMAP MF_00008
Subcellular location	Cytoplasm By similarity HAMAP MF_00008.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thymidylate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 264	264	Thymidylate synthase HAMAP MF_00008		PRO_1000000670
Sites
	Active site	146	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q12KG8 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: CE25FB897888D4DD	FASTA	264	30,183
	10 20 30 40 50 60 MKQYLALMEH ILAHGAVKTD RTGTGTRSVF GYQMRFDLNQ GFPLVTTKKC HLRSIIHELL 70 80 90 100 110 120 WFLQGDTNVA YLHEHNVSIW DEWADDKGNL GPVYGAQWRS WPTQSGKAVD QISQIIEQIK 130 140 150 160 170 180 TQPDSRRLIV SAWNVGELDK MALAPCHALF QFYVADGKLS CQLYQRSCDV FLGLPFNIAS 190 200 210 220 230 240 YALLTMMVAQ QCDLALGDFV WTGGDTHLYS NHMEQTKLQL SREPHALPQM NILRKPESIF 250 260 DYQFDDFELV NYQPHPHIKA PVAI

References

[1]

"Complete sequence of Shewanella denitrificans OS217." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: OS217 / ATCC BAA-1090 / DSM 15013.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000302 Genomic DNA. Translation: ABE56058.1.
RefSeq	YP_563781.1. NC_007954.1.
3D structure databases
ProteinModelPortal	Q12KG8.
SMR	Q12KG8. Positions 1-264.
ModBase	Search...
Protein-protein interaction databases
STRING	Q12KG8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4019298.
GenomeReviews	Gene locus Sden_2779 in contig CP000302_GR.
KEGG	sdn:Sden_2779.
NMPDR	fig|318161.14.peg.2701.
PATRIC	23491568. VBISheDen79529_2918.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0207.
HOGENOM	HBG588098.
OMA	DIPRMKL.
PhylomeDB	Q12KG8.
ProtClustDB	PRK01827.
Enzyme and pathway databases
BioCyc	SDEN318161:SDEN_2779-MONOMER.
Family and domain databases
HAMAP	MF_00008. Thymidy_synth_bact. [Tree]
InterPro	IPR023451. Thymidate_synth/dCMP_Mease. IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
KO	K00560.
PANTHER	PTHR11549:SF2. Thymidylat_synth_C. 1 hit.
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
SUPFAM	SSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMs	TIGR03284. Thym_sym. 2 hits.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_SHEDO

Accession

Primary (citable) accession number: Q12KG8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 22, 2006
Last modified:	January 25, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program