Skip Header

Contribute Send feedback
Read comments (?) or add your own

Q12KE6 (MTNN_SHEDO) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
Short name=MTA/SAH nucleosidase
Short name=MTAN
EC=3.2.2.9
Alternative name(s):
5'-methylthioadenosine nucleosidase
Short name=MTA nucleosidase
S-adenosylhomocysteine nucleosidase
Short name=AdoHcy nucleosidase
Short name=SAH nucleosidase
Short name=SRH nucleosidase
Gene names
Name:mtnN
Ordered Locus Names:Sden_2801
OrganismShewanella denitrificans (strain OS217 / ATCC BAA-1090 / DSM 15013) [Complete proteome] [HAMAP]
Taxonomic identifier318161 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length230 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively By similarity. HAMAP MF_01684

Catalytic activity

S-adenosyl-L-homocysteine + H2O = S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine. HAMAP MF_01684

S-methyl-5'-thioadenosine + H2O = S-methyl-5-thio-D-ribose + adenine. HAMAP MF_01684

Pathway

Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (hydrolase route): step 1/2. HAMAP MF_01684

Sequence similarities

Belongs to the PNP/UDP phosphorylase family. MtnN subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 2302305'-methylthioadenosine/S-adenosylhomocysteine nucleosidase HAMAP MF_01684
PRO_0000359343

Regions

Region174 – 1752Substrate binding By similarity

Sites

Active site121Proton acceptor By similarity
Binding site781Substrate; via amide nitrogen By similarity
Binding site1531Substrate; via amide nitrogen and carbonyl oxygen By similarity
Binding site1981Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q12KE6 [UniParc].

Last modified August 22, 2006. Version 1.
Checksum: 08FA0C3D40CB5B6B

FASTA23024,183
        10         20         30         40         50         60 
MKIGIIGAME PEVAHLIQSL TSAEHSLIAG IEFISGQIAG KDVVITRSGI GKVAASIATT 

        70         80         90        100        110        120 
LLIEKFAVTQ VVNTGSAGGF VDSLKIGDIV ISSEVRHHDV DVTAFGYEIG QMAQQPAAFI 

       130        140        150        160        170        180 
PDAALVEAAK KAVSALGEVK AIEGLICTGD SFICDPERTK VMRANFPTMA ACEMEGAAIA 

       190        200        210        220        230 
QVCHQFKVPF VVIRSLSDNA NNDSPVDFDS YIIKAGHHSA MMVVALLTEL

« Hide

References

[1]"Complete sequence of Shewanella denitrificans OS217."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Richardson P.
Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: OS217 / ATCC BAA-1090 / DSM 15013.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000302 Genomic DNA. Translation: ABE56080.1.
RefSeqYP_563803.1. NC_007954.1.

3D structure databases

ProteinModelPortalQ12KE6.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ12KE6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4019320.
GenomeReviewsGene locus Sden_2801 in contig CP000302_GR.
KEGGsdn:Sden_2801.
NMPDRfig|318161.14.peg.2721.
PATRIC23491612. VBISheDen79529_2940.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0775.
HOGENOMHBG367723.
OMAVIGAMEQ.
PhylomeDBQ12KE6.
ProtClustDBCLSK906203.

Enzyme and pathway databases

BioCycSDEN318161:SDEN_2801-MONOMER.

Family and domain databases

HAMAPMF_01684. Salvage_tnN.
[Tree]
InterProIPR010049. MTA_SAH_Nsdase.
IPR018017. Nucleoside_phosphorylase.
IPR000845. Nucleoside_phosphorylase_d.
[Graphical view]
KOK01243.
PANTHERPTHR21234. PNP_UDP. 1 hit.
PTHR21234:SF6. PTHR21234:SF6. 1 hit.
PfamPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
TIGRFAMsTIGR01704. MTA/SAH-Nsdase. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMTNN_SHEDO
AccessionPrimary (citable) accession number: Q12KE6
Entry history
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: August 22, 2006
Last modified: January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents