Q12K11 (RLMG_SHEDO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase G
EC=2.1.1.174

Alternative name(s):
23S rRNA m2G1835 methyltransferase
rRNA (guanine-N(2)-)-methyltransferase RlmG

Gene names

Name:	rlmG
Ordered Locus Names:	Sden_2937

Organism

Shewanella denitrificans (strain OS217 / ATCC BAA-1090 / DSM 15013) [Complete proteome] [HAMAP]

Taxonomic identifier

318161 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	383 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA By similarity. HAMAP MF_01859
Catalytic activity	S-adenosyl-L-methionine + guanosine(1835) in 23S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanosine(1835) in 23S rRNA. HAMAP MF_01859
Subcellular location	Cytoplasm Potential HAMAP MF_01859.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmG family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro rRNA (guanine-N2-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 383	383	Ribosomal RNA large subunit methyltransferase G HAMAP MF_01859		PRO_0000366508

Sequences

	Sequence		Length	Mass (Da)
	Q12K11 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 5E80DE4C41405037	FASTA	383	41,876
	10 20 30 40 50 60 MTSQFSTAGL TLELLRYPSQ QVSNLQAWDA ADEHIIKVLA EKVLEENAVP PQPCAIINDS 70 80 90 100 110 120 FGALSCALTR MDSSWPLTVI SDAKTSQLGA LENLTRNQLD AEGIKWLTSR APLPQDLSLV 130 140 150 160 170 180 LMKLPKNLAY FAQQLRGLSQ VLPQGTQVLI GAKAKSINSA LITLLAEHLG PASASLAWKN 190 200 210 220 230 240 TRVITCVSDG RARSLPKAIT WSVPEYQLEI SNLSNVFAAN KLDIGARIML ENMPNGEFNT 250 260 270 280 290 300 VVDLGCGNGI LGLRAAGLYP KAKIHFIDDS EMAVASSQAN WALNTLAPER AEFHWDDCMS 310 320 330 340 350 360 HLDETVQPDL VLCNPPFHQG EAITDHIAWQ MFNDAKHRLR SGGILHIVGN RHLNYHVKLK 370 380 RLFGNCTTVA SNGKFVILQS VKG

References

[1]

"Complete sequence of Shewanella denitrificans OS217." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: OS217 / ATCC BAA-1090 / DSM 15013.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000302 Genomic DNA. Translation: ABE56215.1.
RefSeq	YP_563938.1. NC_007954.1.
3D structure databases
ProteinModelPortal	Q12K11.
ModBase	Search...
Protein-protein interaction databases
STRING	Q12K11.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4019462.
GenomeReviews	Gene locus Sden_2937 in contig CP000302_GR.
KEGG	sdn:Sden_2937.
NMPDR	fig|318161.14.peg.2849.
PATRIC	23491910. VBISheDen79529_3083.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2813.
HOGENOM	HBG321931.
OMA	NRHLGYH.
PhylomeDB	Q12K11.
ProtClustDB	CLSK906089.
Enzyme and pathway databases
BioCyc	SDEN318161:SDEN_2937-MONOMER.
Family and domain databases
HAMAP	MF_01859. 23SrRNA_methyltr_G. [Tree]
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. IPR017237. rRNA_m2G-MeTrfase_RsmD. IPR007848. Small_mtfrase. [Graphical view]
KO	K00564.
Pfam	PF05175. MTS. 1 hit. [Graphical view]
PIRSF	PIRSF037565. RRNA_m2G_Mtase_RsmD_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMG_SHEDO

Accession

Primary (citable) accession number: Q12K11

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	August 22, 2006
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program