Q12ID7 (PLSB_SHEDO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol-3-phosphate acyltransferase
Short name=GPAT
EC=2.3.1.15

Gene names

Name:	plsB
Ordered Locus Names:	Sden_3514

Organism

Shewanella denitrificans (strain OS217 / ATCC BAA-1090 / DSM 15013) [Complete proteome] [HAMAP]

Taxonomic identifier

318161 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	807 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. HAMAP MF_00393
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3. HAMAP MF_00393
Subcellular location	Cell inner membrane; Peripheral membrane protein; Cytoplasmic side By similarity HAMAP MF_00393.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity. HAMAP MF_00393
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 807	807	Glycerol-3-phosphate acyltransferase HAMAP MF_00393		PRO_1000049456
Regions
	Motif	308 – 313	6	HXXXXD motif HAMAP MF_00393

Sequences

	Sequence		Length	Mass (Da)
	Q12ID7 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: C582FD38ECB0542C	FASTA	807	91,643
	10 20 30 40 50 60 MSNYDSIFIK SLRWIQKWLV QTIVVPHDPL TDLNLDPSKP VVYVMKTESI SDLAALSGIT 70 80 90 100 110 120 ANFGLPSPYD PLTLDKVSIA RVVCLEGRKP MIGQREGGEK FLDSFTQLLA LHKQDKQLDI 130 140 150 160 170 180 QLVPVSLYWG RTPGKEDDTM KAAVFERENP TWLRKFFMIL FLGRHNFVQF SNAVSLRHMA 190 200 210 220 230 240 DEHGTDKSIA HKLVRVARVH FRRQRKVMTG PLLPNRQALF NALLKSESIK KAIEEEATNK 250 260 270 280 290 300 KISIEKARET AIVYLDEIAA DYSDSLVRIT ERFLTWLWNK LYSGINIERA EQVRQLHHDG 310 320 330 340 350 360 HEIVYVPCHR SHMDYLLISY ILYYQGMVPP HIAAGINLNF WPAGPMFRRG GAFFIRRSFN 370 380 390 400 410 420 GNKLYTAVFR EYLDQLFAKG YSVEYFTEGG RSRTGRLLAP KTGMIAMTMN SVLRGIERPV 430 440 450 460 470 480 TLVPVYLGYD HVMEVATYHK ELSGKKKQKE SLWQVFGAIR KLGNFGQGYV NFGEPINMQN 490 500 510 520 530 540 FLTEQAPEWR AELAKDPEQK PSWFTPAVNV LANRVMTNIN GAAAASSVTL TSLILLASEQ 550 560 570 580 590 600 NALERTQLER QLDLYLNLLK KVPYTPFTSV AQGDSQQVVD HCLGLNKFIS TRDALGEIIS 610 620 630 640 650 660 IDPKIAVTMS YYRNNIIHLM VVPSLIASCL VQYEVRSRSE IKAIVNDFYP LLKAELFMGI 670 680 690 700 710 720 ADLDSYIDDI IDLLIEEQLV EESAGLSIVE SHISQLWLMA QTVSETLQRY AIIFNLLAHK 730 740 750 760 770 780 PNVERADLEN DSHLLAQRLG ALHGITAPEF YDKKLYNTLS VKLKELGYLS AVEKQVDVAR 790 800 IRDHANGLLW SSVRQTIIDS VAAEHGH

References

[1]

"Complete sequence of Shewanella denitrificans OS217." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: OS217 / ATCC BAA-1090 / DSM 15013.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000302 Genomic DNA. Translation: ABE56789.1.
RefSeq	YP_564512.1. NC_007954.1.
3D structure databases
ProteinModelPortal	Q12ID7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q12ID7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4020068.
GenomeReviews	Gene locus Sden_3514 in contig CP000302_GR.
KEGG	sdn:Sden_3514.
NMPDR	fig|318161.14.peg.3409.
PATRIC	23493140. VBISheDen79529_3688.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2937.
HOGENOM	HBG296590.
OMA	WNKLYQG.
ProtClustDB	PRK04974.
Enzyme and pathway databases
BioCyc	SDEN318161:SDEN_3514-MONOMER.
Family and domain databases
HAMAP	MF_00393. Glyc3P_acyltrans. [Tree]
InterPro	IPR002123. Acyltransferase. IPR022284. G3P_O-AcylTrfase. [Graphical view]
KO	K00631.
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
PIRSF	PIRSF000437. GPAT_DHAPAT. 1 hit.
SMART	SM00563. PlsC. 1 hit. [Graphical view]
TIGRFAMs	TIGR03703. PlsB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PLSB_SHEDO

Accession

Primary (citable) accession number: Q12ID7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 22, 2006
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program