Q12HF2 (MNMG_POLSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG

Alternative name(s):
Glucose-inhibited division protein A

Gene names

Name:	mnmG
Synonyms:	gidA
Ordered Locus Names:	Bpro_0073

Organism

Polaromonas sp. (strain JS666 / ATCC BAA-500) [Complete proteome] [HAMAP]

Taxonomic identifier

296591 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Polaromonas

Protein attributes

Sequence length	673 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34 By similarity. HAMAP-Rule MF_00129
Cofactor	FAD By similarity.
Subunit structure	Homodimer By similarity. Heterotetramer of two MnmE and two MnmG subunits By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the MnmG family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NAD
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tRNA wobble uridine modification Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	flavin adenine dinucleotide binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 673	673	tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG HAMAP-Rule MF_00129		PRO_0000345316
Regions
	Nucleotide binding	17 – 22	6	FAD By similarity
	Nucleotide binding	284 – 298	15	NAD Potential

Sequences

	Sequence		Length	Mass (Da)
	Q12HF2 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 22AA1F52519A4970	FASTA	673	74,027
	10 20 30 40 50 60 MQSPYIYPQE FDVIVVGGGH AGTEAALASA RMGCKTLLLS HNIETLGQMS CNPSIGGIGK 70 80 90 100 110 120 GHLVKEVDAM GGAMALATDE GGIQFRILNS SKGPAVRATR AQADRILYKA AIRRRLENQP 130 140 150 160 170 180 NLWLFQQAVD DLMVEGDRVV GAVTQVGIRF RSRTVVLTAG TFLDGKIHVG LNNYAAGRAG 190 200 210 220 230 240 DPPAVSLSSR LKELKLPQGR LKTGTPPRID GRTIDFSKCI EQPGDGMPGG TAGPVPVFSF 250 260 270 280 290 300 MGGAIPHPQQ MPCWITHTNE RTHEIIRSGF DRSPMFTGKI DGVGPRYCPS VEDKINRFAD 310 320 330 340 350 360 KESHQIFLEP EGLTTHEIYP NGISTSLPFD IQYELVRSMA GMENAHILRP GYAIEYDYFD 370 380 390 400 410 420 PRALKTTFET RAIGGLFFAG QINGTTGYEE AAAQGMFAGI NAALQCRALG GLPNDHGGAW 430 440 450 460 470 480 LPRRDEAYLG VLVDDLITKG VTEPYRMFTS RAEYRLMLRE DNADMRLTEK GRELGLVDDA 490 500 510 520 530 540 RWDAFSRKRD AVSRETERLR SLWVNPHNLP LAEAERVLGK SIEREYNLLD LLRRPDVNYA 550 560 570 580 590 600 GLMSLEEGKY ANPELAAEAA ASDDLAKSVI EQIEITAKYA GYIDLQKTEV ERAAHYENLK 610 620 630 640 650 660 LPTDLDYLQV SALSFEARQT LARHRPETLG MASRISGITP ATVSLLLVHL KKNLWKNTVP 670 LKTTDTSTEK AQA

References

[1]

"Complete sequence of chromosome of Polaromonas sp. JS666." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Munk A.C., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Anderson I., Richardson P. Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JS666 / ATCC BAA-500.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000316 Genomic DNA. Translation: ABE42040.1.
RefSeq	YP_546938.1. NC_007948.1.
3D structure databases
ProteinModelPortal	Q12HF2.
SMR	Q12HF2. Positions 6-584.
ModBase	Search...
Protein-protein interaction databases
STRING	296591.Bpro_0073.
Proteomic databases
PRIDE	Q12HF2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABE42040; ABE42040; Bpro_0073.
GeneID	4011075.
KEGG	pol:Bpro_0073.
PATRIC	22953176. VBIPolSp102244_0077.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0445.
HOGENOM	HOG000201059.
KO	K03495.
OMA	AQMSCNP.
ProtClustDB	PRK05192.
Enzyme and pathway databases
BioCyc	PSP296591:GHI4-794-MONOMER.
Family and domain databases
HAMAP	MF_00129. MnmG_GidA.
InterPro	IPR004416. GidA. IPR026904. GidA-assoc_3. IPR002218. GIDA-rel. IPR020595. GIDA-rel_CS. [Graphical view]
Pfam	PF01134. GIDA. 1 hit. PF13932. GIDA_assoc_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00136. gidA. 1 hit.
PROSITE	PS01280. GIDA_1. 1 hit. PS01281. GIDA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MNMG_POLSJ

Accession

Primary (citable) accession number: Q12HF2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	August 22, 2006
Last modified:	May 1, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program