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Q12HF2 (MNMG_POLSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG
Alternative name(s):
Glucose-inhibited division protein A
Gene names
Name:mnmG
Synonyms:gidA
Ordered Locus Names:Bpro_0073
OrganismPolaromonas sp. (strain JS666 / ATCC BAA-500) [Complete proteome] [HAMAP]
Taxonomic identifier296591 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaePolaromonas

Protein attributes

Sequence length673 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34 By similarity. HAMAP-Rule MF_00129

Cofactor

FAD By similarity. HAMAP-Rule MF_00129

Subunit structure

Homodimer By similarity. Heterotetramer of two MnmE and two MnmG subunits By similarity. HAMAP-Rule MF_00129

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00129.

Sequence similarities

Belongs to the MnmG family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   LigandFAD
Flavoprotein
NAD
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtRNA wobble uridine modification

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionflavin adenine dinucleotide binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 673673tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG HAMAP-Rule MF_00129
PRO_0000345316

Regions

Nucleotide binding17 – 226FAD By similarity
Nucleotide binding284 – 29815NAD Potential

Sequences

Sequence LengthMass (Da)Tools
Q12HF2 [UniParc].

Last modified August 22, 2006. Version 1.
Checksum: 22AA1F52519A4970

FASTA67374,027
        10         20         30         40         50         60 
MQSPYIYPQE FDVIVVGGGH AGTEAALASA RMGCKTLLLS HNIETLGQMS CNPSIGGIGK 

        70         80         90        100        110        120 
GHLVKEVDAM GGAMALATDE GGIQFRILNS SKGPAVRATR AQADRILYKA AIRRRLENQP 

       130        140        150        160        170        180 
NLWLFQQAVD DLMVEGDRVV GAVTQVGIRF RSRTVVLTAG TFLDGKIHVG LNNYAAGRAG 

       190        200        210        220        230        240 
DPPAVSLSSR LKELKLPQGR LKTGTPPRID GRTIDFSKCI EQPGDGMPGG TAGPVPVFSF 

       250        260        270        280        290        300 
MGGAIPHPQQ MPCWITHTNE RTHEIIRSGF DRSPMFTGKI DGVGPRYCPS VEDKINRFAD 

       310        320        330        340        350        360 
KESHQIFLEP EGLTTHEIYP NGISTSLPFD IQYELVRSMA GMENAHILRP GYAIEYDYFD 

       370        380        390        400        410        420 
PRALKTTFET RAIGGLFFAG QINGTTGYEE AAAQGMFAGI NAALQCRALG GLPNDHGGAW 

       430        440        450        460        470        480 
LPRRDEAYLG VLVDDLITKG VTEPYRMFTS RAEYRLMLRE DNADMRLTEK GRELGLVDDA 

       490        500        510        520        530        540 
RWDAFSRKRD AVSRETERLR SLWVNPHNLP LAEAERVLGK SIEREYNLLD LLRRPDVNYA 

       550        560        570        580        590        600 
GLMSLEEGKY ANPELAAEAA ASDDLAKSVI EQIEITAKYA GYIDLQKTEV ERAAHYENLK 

       610        620        630        640        650        660 
LPTDLDYLQV SALSFEARQT LARHRPETLG MASRISGITP ATVSLLLVHL KKNLWKNTVP 

       670 
LKTTDTSTEK AQA 

« Hide

References

[1]"Complete sequence of chromosome of Polaromonas sp. JS666."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Munk A.C., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Anderson I., Richardson P.
Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JS666 / ATCC BAA-500.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000316 Genomic DNA. Translation: ABE42040.1.
RefSeqYP_546938.1. NC_007948.1.

3D structure databases

ProteinModelPortalQ12HF2.
SMRQ12HF2. Positions 6-584.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING296591.Bpro_0073.

Proteomic databases

PRIDEQ12HF2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABE42040; ABE42040; Bpro_0073.
GeneID4011075.
KEGGpol:Bpro_0073.
PATRIC22953176. VBIPolSp102244_0077.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0445.
HOGENOMHOG000201059.
KOK03495.
OMANPQIECG.
OrthoDBEOG6W9X6J.

Enzyme and pathway databases

BioCycPSP296591:GHI4-794-MONOMER.

Family and domain databases

HAMAPMF_00129. MnmG_GidA.
InterProIPR004416. GidA.
IPR026904. GidA-assoc_3.
IPR002218. GIDA-rel.
IPR020595. GIDA-rel_CS.
[Graphical view]
PfamPF01134. GIDA. 1 hit.
PF13932. GIDA_assoc_3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00136. gidA. 1 hit.
PROSITEPS01280. GIDA_1. 1 hit.
PS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMNMG_POLSJ
AccessionPrimary (citable) accession number: Q12HF2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: August 22, 2006
Last modified: May 14, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families