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Q12F39

- BIOB1_POLSJ

UniProt

Q12F39 - BIOB1_POLSJ

Protein

Biotin synthase 1

Gene

bioB1

Organism
Polaromonas sp. (strain JS666 / ATCC BAA-500)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 1 (22 Aug 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi72 – 721Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi76 – 761Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi79 – 791Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi116 – 1161Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi147 – 1471Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi207 – 2071Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi279 – 2791Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciPSP296591:GHI4-1618-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthase 1UniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioB1UniRule annotation
    Ordered Locus Names:Bpro_0897
    OrganismiPolaromonas sp. (strain JS666 / ATCC BAA-500)
    Taxonomic identifieri296591 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaePolaromonas
    ProteomesiUP000001983: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 337337Biotin synthase 1PRO_0000381529Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi296591.Bpro_0897.

    Structurei

    3D structure databases

    ProteinModelPortaliQ12F39.
    SMRiQ12F39. Positions 25-330.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239957.
    KOiK01012.
    OMAiCTELATR.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q12F39-1 [UniParc]FASTAAdd to Basket

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    MSSVLTQPLA FHPPRPAVQP REHGRWSVDE IEALFNLPFP ELMHRAQTVH    50
    RENFDPTRVE FATLLSVKTG GCAEDCGYCP QAARYDTGVE ASKLMEPAEV 100
    LAAAQRAQAA GATRFCMGAA WRSPKDRDIE KVAELVRTVK ALGLETCATL 150
    GMLEDGHAQT LQSAGLDYYN HNLDSAPDFY GDIITTRDYQ DRLDTLARVR 200
    SAGVKVCCGG IVGMGETRRQ RAGLVAELAN LTPYPESVPI NNLVKVEGTP 250
    LANQADLDPF EFVRTIAVAR ITMPTARVRL SAGRQQMGDG VQALCFLAGA 300
    NSIFYGDKLL TTGNPDTEAD VTLLQRLGMS RSAPDVR 337
    Length:337
    Mass (Da):36,665
    Last modified:August 22, 2006 - v1
    Checksum:i1977396CC919C26B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000316 Genomic DNA. Translation: ABE42853.1.
    RefSeqiWP_011481855.1. NC_007948.1.
    YP_547751.1. NC_007948.1.

    Genome annotation databases

    EnsemblBacteriaiABE42853; ABE42853; Bpro_0897.
    GeneIDi4011205.
    KEGGipol:Bpro_0897.
    PATRICi22954868. VBIPolSp102244_0919.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000316 Genomic DNA. Translation: ABE42853.1 .
    RefSeqi WP_011481855.1. NC_007948.1.
    YP_547751.1. NC_007948.1.

    3D structure databases

    ProteinModelPortali Q12F39.
    SMRi Q12F39. Positions 25-330.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 296591.Bpro_0897.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABE42853 ; ABE42853 ; Bpro_0897 .
    GeneIDi 4011205.
    KEGGi pol:Bpro_0897.
    PATRICi 22954868. VBIPolSp102244_0919.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239957.
    KOi K01012.
    OMAi CTELATR.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci PSP296591:GHI4-1618-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: JS666 / ATCC BAA-500.

    Entry informationi

    Entry nameiBIOB1_POLSJ
    AccessioniPrimary (citable) accession number: Q12F39
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: August 22, 2006
    Last modified: October 1, 2014
    This is version 58 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3