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Protein

Biotin synthase 1

Gene

bioB1

Organism
Polaromonas sp. (strain JS666 / ATCC BAA-500)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
  • [2Fe-2S] clusterUniRule annotationNote: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi: biotin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes biotin from 7,8-diaminononanoate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. ATP-dependent dethiobiotin synthetase BioD (bioD), ATP-dependent dethiobiotin synthetase BioD (bioD)
  2. Biotin synthase 1 (bioB1), Biotin synthase (bioB), Biotin synthase 2 (bioB2)
This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes biotin from 7,8-diaminononanoate, the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi72Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi76Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi79Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi116Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi147Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi207Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi279Iron-sulfur 2 (2Fe-2S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processBiotin biosynthesis
Ligand2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciPSP296591:G1G6E-900-MONOMER
UniPathwayiUPA00078; UER00162

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase 1UniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioB1UniRule annotation
Ordered Locus Names:Bpro_0897
OrganismiPolaromonas sp. (strain JS666 / ATCC BAA-500)
Taxonomic identifieri296591 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaePolaromonas
Proteomesi
  • UP000001983 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003815291 – 337Biotin synthase 1Add BLAST337

Proteomic databases

PRIDEiQ12F39

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi296591.Bpro_0897

Structurei

3D structure databases

ProteinModelPortaliQ12F39
SMRiQ12F39
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZF Bacteria
COG0502 LUCA
HOGENOMiHOG000239957
KOiK01012
OMAiCTERSEQ
OrthoDBiPOG091H01DF

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_01694 BioB, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR010722 BATS_dom
IPR034416 BATS_domain_containing
IPR002684 Biotin_synth/BioAB
IPR024177 Biotin_synthase
IPR006638 Elp3/MiaB/NifB
IPR007197 rSAM
PANTHERiPTHR22976 PTHR22976, 1 hit
PfamiView protein in Pfam
PF06968 BATS, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF001619 Biotin_synth, 1 hit
SFLDiSFLDF00272 biotin_synthase, 1 hit
SFLDG01060 BATS_domain_containing, 1 hit
SFLDG01278 biotin_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00876 BATS, 1 hit
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00433 bioB, 1 hit

Sequencei

Sequence statusi: Complete.

Q12F39-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSVLTQPLA FHPPRPAVQP REHGRWSVDE IEALFNLPFP ELMHRAQTVH
60 70 80 90 100
RENFDPTRVE FATLLSVKTG GCAEDCGYCP QAARYDTGVE ASKLMEPAEV
110 120 130 140 150
LAAAQRAQAA GATRFCMGAA WRSPKDRDIE KVAELVRTVK ALGLETCATL
160 170 180 190 200
GMLEDGHAQT LQSAGLDYYN HNLDSAPDFY GDIITTRDYQ DRLDTLARVR
210 220 230 240 250
SAGVKVCCGG IVGMGETRRQ RAGLVAELAN LTPYPESVPI NNLVKVEGTP
260 270 280 290 300
LANQADLDPF EFVRTIAVAR ITMPTARVRL SAGRQQMGDG VQALCFLAGA
310 320 330
NSIFYGDKLL TTGNPDTEAD VTLLQRLGMS RSAPDVR
Length:337
Mass (Da):36,665
Last modified:August 22, 2006 - v1
Checksum:i1977396CC919C26B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000316 Genomic DNA Translation: ABE42853.1
RefSeqiWP_011481855.1, NC_007948.1

Genome annotation databases

EnsemblBacteriaiABE42853; ABE42853; Bpro_0897
KEGGipol:Bpro_0897

Similar proteinsi

Entry informationi

Entry nameiBIOB1_POLSJ
AccessioniPrimary (citable) accession number: Q12F39
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: August 22, 2006
Last modified: May 23, 2018
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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