Reviewed, UniProtKB/Swiss-Prot Q12EQ4 (HUTI_POLSJ)

Names and origin

Protein names

Recommended name:
    Imidazolonepropionase
    EC=3.5.2.7
Alternative name(s):
    Imidazolone-5-propionate hydrolase

Gene names

Name:	hutI
Ordered Locus Names:	Bpro_1035

Organism

Polaromonas sp. (strain JS666 / ATCC BAA-500) [Complete proteome] [HAMAP]

Taxonomic identifier

296591 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Polaromonas

Protein attributes

Sequence length	425 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H2O = N-formimidoyl-L-glutamate + H+. HAMAP MF_00372
Cofactor	Binds 1 zinc or iron ion per subunit By similarity.
Pathway	Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 3/3. HAMAP MF_00372
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the hutI family.

Ontologies

Keywords
Biological process	Histidine metabolism
Cellular component	Cytoplasm
Ligand	Iron Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	histidine catabolic process to glutamate and formamide Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	imidazolonepropionase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 425	425	Imidazolonepropionase HAMAP MF_00372		PRO_0000306481
Sites
	Metal binding	78	1	Zinc or iron By similarity
	Metal binding	80	1	Zinc or iron By similarity
	Metal binding	248	1	Zinc or iron By similarity
	Metal binding	323	1	Zinc or iron By similarity
	Binding site	87	1	Substrate By similarity
	Binding site	100	1	Substrate By similarity
	Binding site	150	1	Substrate By similarity
	Binding site	183	1	Substrate By similarity
	Binding site	251	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q12EQ4-1 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 1D52F947C74FA752	FASTA	425	44,819
	10 20 30 40 50 60 MTTAPHPAAD GIWEHLRLMP GALADDSPVA TNTEAAIVVT EGRIRWIGAS AALPAGFSAL 70 80 90 100 110 120 PRFDGGGALV TPGLVDCHTH LVYGGQRANE FAMRLAGASY EEVAKAGGGI VSSVRATRAA 130 140 150 160 170 180 GEDELFAQAA PRLEQLLADG VCAIEIKSGY GLALEHERKQ LRVARRLGEA YGVTVRTTFL 190 200 210 220 230 240 GAHALPPEYA GRSQDYIDLV CREMLPALAA EGLVDAVDVF CERIAFSLSE TEQVFQAAQR 250 260 270 280 290 300 LGLPVKLHAE QLSDMGGAAL AARYGALSCD HIEHLSQAGI DAMRAAGTVA VLLPGAYYTL 310 320 330 340 350 360 RDTHLPPIAA LREAGVPMAV STDHNPGTSP ALSLLLMANM ACTLFRLTVP EALAGITRHA 370 380 390 400 410 420 ARALGLQDTH GALGVGRPAN FVLWQLNDSA ELAYWLGQQA PRTIVRQGRV ALDGLQIAPN ARITP

References

[1]

"Complete sequence of chromosome of Polaromonas sp. JS666." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Munk A.C., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Anderson I., Richardson P. Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000316 Genomic DNA. Translation: ABE42988.1.
RefSeq	YP_547886.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4012156.
GenomeReviews	Gene locus Bpro_1035 in contig CP000316_GR.
KEGG	pol:Bpro_1035.
NMPDR	fig|296591.1.peg.3371.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q12EQ4.
OMA	Q12EQ4. MNMACTL.
Enzyme and pathway databases
BioCyc	PSP296591:BPRO_1035-MON.
Family and domain databases
HAMAP	MF_00372. [Tree]
InterPro	IPR013108. Amidohydro_3. IPR005920. HutI. [Graphical view]
Pfam	PF07969. Amidohydro_3. 1 hit. [Graphical view]
ProDom	PD001248. Amidohydro_like. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01224. hutI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HUTI_POLSJ

Accession

Primary (citable) accession number: Q12EQ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	August 22, 2006
Last modified:	June 16, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)