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Q12D73

- BIOB2_POLSJ

UniProt

Q12D73 - BIOB2_POLSJ

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Protein

Biotin synthase 2

Gene

bioB2

Organism
Polaromonas sp. (strain JS666 / ATCC BAA-500)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi73 – 731Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi77 – 771Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi80 – 801Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi117 – 1171Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi148 – 1481Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi208 – 2081Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi280 – 2801Iron-sulfur 2 (2Fe-2S)UniRule annotation

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciPSP296591:GHI4-2301-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase 2UniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioB2UniRule annotation
Ordered Locus Names:Bpro_1580
OrganismiPolaromonas sp. (strain JS666 / ATCC BAA-500)
Taxonomic identifieri296591 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaePolaromonas
ProteomesiUP000001983: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 343343Biotin synthase 2PRO_0000381530Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi296591.Bpro_1580.

Structurei

3D structure databases

ProteinModelPortaliQ12D73.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OMAiDETQALC.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q12D73 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSIATIPVSS LRRAPAPTTA PQAPLRWQVE DIAALYELPF MDLLFRAQQV
60 70 80 90 100
HREHFDANEV QLSTLLSIKT GGCAEDCGYC PQSTHFETEV KASKLMPLAE
110 120 130 140 150
VIEAARAAKD QGATRFCMGA AWRSPKERDM ERVTEIVREV RSLGLETCMT
160 170 180 190 200
LGMLQAEQAQ ALKDAGLDYY NHNLDSAPEF YGDIISTRTY QDRLDTLGHV
210 220 230 240 250
RQAGINVCCG GIVGMGESRL QRAGLIAQLA NLSPYPESVP INNLVPVAGT
260 270 280 290 300
PLANTAPLDP FEFVRTIAVA RITMPLTMVR LSAGREQMDE ALQALCFAAG
310 320 330 340
ANSIFYGDKL LTTSNPQADR DRQLFERLGL KTQGARPAAQ QAQ
Length:343
Mass (Da):37,541
Last modified:July 28, 2009 - v2
Checksum:i51931A0C47219AC8
GO

Sequence cautioni

The sequence ABE43519.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000316 Genomic DNA. Translation: ABE43519.1. Different initiation.
RefSeqiWP_011482518.1. NC_007948.1.
YP_548417.1. NC_007948.1.

Genome annotation databases

EnsemblBacteriaiABE43519; ABE43519; Bpro_1580.
GeneIDi4015782.
KEGGipol:Bpro_1580.
PATRICi22956265. VBIPolSp102244_1609.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000316 Genomic DNA. Translation: ABE43519.1 . Different initiation.
RefSeqi WP_011482518.1. NC_007948.1.
YP_548417.1. NC_007948.1.

3D structure databases

ProteinModelPortali Q12D73.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 296591.Bpro_1580.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABE43519 ; ABE43519 ; Bpro_1580 .
GeneIDi 4015782.
KEGGi pol:Bpro_1580.
PATRICi 22956265. VBIPolSp102244_1609.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OMAi DETQALC.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci PSP296591:GHI4-2301-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JS666 / ATCC BAA-500.

Entry informationi

Entry nameiBIOB2_POLSJ
AccessioniPrimary (citable) accession number: Q12D73
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 28, 2009
Last modified: October 1, 2014
This is version 62 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3