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Q12D45

- SYE_POLSJ

UniProt

Q12D45 - SYE_POLSJ

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Protein
Glutamate--tRNA ligase
Gene
gltX, Bpro_1609
Organism
Polaromonas sp. (strain JS666 / ATCC BAA-500)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity.UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei246 – 2461ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. tRNA binding Source: InterPro

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPSP296591:GHI4-2330-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligase (EC:6.1.1.17)
Alternative name(s):
Glutamyl-tRNA synthetase
Short name:
GluRS
Gene namesi
Name:gltX
Ordered Locus Names:Bpro_1609
OrganismiPolaromonas sp. (strain JS666 / ATCC BAA-500)
Taxonomic identifieri296591 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaePolaromonas
ProteomesiUP000001983: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 462462Glutamate--tRNA ligaseUniRule annotation
PRO_1000001932Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi296591.Bpro_1609.

Structurei

3D structure databases

ProteinModelPortaliQ12D45.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi11 – 2111"HIGH" regionUniRule annotation
Add
BLAST
Motifi243 – 2475"KMSKS" regionUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252722.
KOiK01885.
OMAiHCLRASI.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12D45-1 [UniParc]FASTAAdd to Basket

« Hide

MTQKIRTRFA PSPTGFIHLG NIRSALYPWA FARATGGDFI LRIEDTDVER    50
SSQAAVDVII EGMRWLGLDH DEGPFYQMQR MDRYKAVLAE MQAAGQVYPC 100
YMSVAELDAL REKQMAAKEK PRYDGTWRPE PGKTLPPIPA GVQPVLRFKN 150
PQGGSVVWDD KVKGRIEISN DELDDLVIAR PDGTPTYNFC VVVDDIDMAI 200
THVIRGDDHV NNTPRQINIF RALGKDVPVY AHLPTVLNEQ GEKMSKRNGA 250
KPVTQYAAEG YLPDAMVNYL ARLGWSHGDD EIFSREQFLK WFNLDHLGKS 300
AAQFDEAKLR WVNAQHLKAT ADDKLAELVQ PFLAARGVGL DTARMVGGCG 350
LFKDRCSTLV ELADWLQLLV TGGQPSAQDI STHVTEAVRP ALGKLADALV 400
ACEWSKAAIA AAIKQVLAET GLKMPQLAMA VRVLVMGTPQ TPSLDAILEL 450
MKREDVISRL KI 462
Length:462
Mass (Da):51,204
Last modified:August 22, 2006 - v1
Checksum:i99E311B56CE01355
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000316 Genomic DNA. Translation: ABE43547.1.
RefSeqiWP_011482546.1. NC_007948.1.
YP_548445.1. NC_007948.1.

Genome annotation databases

EnsemblBacteriaiABE43547; ABE43547; Bpro_1609.
GeneIDi4015739.
KEGGipol:Bpro_1609.
PATRICi22956323. VBIPolSp102244_1637.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000316 Genomic DNA. Translation: ABE43547.1 .
RefSeqi WP_011482546.1. NC_007948.1.
YP_548445.1. NC_007948.1.

3D structure databases

ProteinModelPortali Q12D45.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 296591.Bpro_1609.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABE43547 ; ABE43547 ; Bpro_1609 .
GeneIDi 4015739.
KEGGi pol:Bpro_1609.
PATRICi 22956323. VBIPolSp102244_1637.

Phylogenomic databases

eggNOGi COG0008.
HOGENOMi HOG000252722.
KOi K01885.
OMAi HCLRASI.
OrthoDBi EOG6DRPF7.

Enzyme and pathway databases

BioCyci PSP296591:GHI4-2330-MONOMER.

Family and domain databases

Gene3Di 1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPi MF_00022_B. Glu_tRNA_synth_B.
InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
PANTHERi PTHR10119. PTHR10119. 1 hit.
Pfami PF00749. tRNA-synt_1c. 1 hit.
[Graphical view ]
PRINTSi PR00987. TRNASYNTHGLU.
SUPFAMi SSF48163. SSF48163. 1 hit.
TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JS666 / ATCC BAA-500.

Entry informationi

Entry nameiSYE_POLSJ
AccessioniPrimary (citable) accession number: Q12D45
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 22, 2006
Last modified: September 3, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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