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Reviewed, UniProtKB/Swiss-Prot Q12CM2 (HLDD_POLSJ)

Last modified June 16, 2009. Version 21. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    ADP-L-glycero-D-manno-heptose-6-epimerase
    EC=5.1.3.20
Alternative name(s):
    ADP-L-glycero-beta-D-manno-heptose-6-epimerase
      Short name=ADP-glyceromanno-heptose 6-epimerase
      Short name=ADP-hep 6-epimerase
      Short name=AGME
Gene names
Name: hldD
Ordered Locus Names: Bpro_1784
OrganismPolaromonas sp. (strain JS666 / ATCC BAA-500) [Complete proteome] [HAMAP]
Taxonomic identifier296591 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Polaromonas

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Protein attributes

Sequence length335 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Catalyzes the interconversion between ADP-D-glycero-beta-D-manno-heptose and ADP-L-glycero-beta-D-manno-heptose via an epimerization at carbon 6 of the heptose By similarity.

Catalytic activity

ADP-D-glycero-D-manno-heptose = ADP-L-glycero-D-manno-heptose. HAMAP MF_01601

Cofactor

Binds 1 NADP+ per subunit By similarity.

Pathway

Nucleotide-sugar biosynthesis; ADP-L-glycero-beta-D-manno-heptose biosynthesis; ADP-L-glycero-beta-D-manno-heptose from D-glycero-beta-D-manno-heptose 7-phosphate: step 4/4. HAMAP MF_01601

Subunit structure

Homopentamer By similarity.

Domain

Contains a large N-terminal NADP-binding domain, and a smaller C-terminal substrate-binding domain By similarity.

Sequence similarities

Belongs to the sugar epimerase family. HldD subfamily.

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Ontologies

Keywords
   Biological processCarbohydrate metabolism
   LigandNADP
   Molecular functionIsomerase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processcarbohydrate metabolic process

Inferred from electronic annotation. Source: HAMAP

cellular metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular functionADP-glyceromanno-heptose 6-epimerase activity

Inferred from electronic annotation. Source: HAMAP

NADP or NADPH binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 335335ADP-L-glycero-D-manno-heptose-6-epimerase HAMAP MF_01601
PRO_0000255736

Regions

Nucleotide binding11 – 122NADP By similarity
Nucleotide binding32 – 332NADP By similarity
Nucleotide binding75 – 795NADP By similarity
Region204 – 2074Substrate binding By similarity

Sites

Active site1391Proton acceptor By similarity
Active site1811Proton acceptor By similarity
Binding site391NADP By similarity
Binding site921NADP By similarity
Binding site1431NADP By similarity
Binding site1721Substrate By similarity
Binding site1731NADP; via amide nitrogen By similarity
Binding site1811NADP By similarity
Binding site1831Substrate; via carbonyl oxygen By similarity
Binding site1901Substrate By similarity
Binding site2171Substrate By similarity
Binding site2961Substrate By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q12CM2-1 [UniParc].

Last modified August 22, 2006. Version 1.
Checksum: E406A44F92E1A782

FASTA33536,962
        10         20         30         40         50         60 
MKIVVTGAAG FIGSNLVKGL NDRGIDDIIA VDDLTHGDKF RNLADLQIAD YIDADDFYDL 

        70         80         90        100        110        120 
FAEGAFGQVE AVFHEGACSD TMELDGKYMM DNNYTLSCEL FHACQEQGTR LLYASSAATY 

       130        140        150        160        170        180 
GGSDTFSESP EFERPLNVYG YSKLLFDQRM RRELGARFEN AATQVAGFRY FNVYGPREQH 

       190        200        210        220        230        240 
KGRMASVAFH QFNQFQAEGK VKLFGDYGGY QAGGQMRDFV FIDDVVAVNL WFLDHPEKSG 

       250        260        270        280        290        300 
IFNLGTGRAQ PFNDVALAVV NTLRQSQNAA AMSLEDAVRG GLIDYITFPP ALVGKYQSYT 

       310        320        330 
QADLQALRAA GCQHAFADVQ TGVAAYMQWL ASAKI

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References

[1]"Complete sequence of chromosome of Polaromonas sp. JS666."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Munk A.C., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Anderson I., Richardson P.
Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases

CP000316 Genomic DNA. Translation: ABE43720.1.
RefSeqYP_548618.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID4015569.
GenomeReviewsGene locus Bpro_1784 in contig CP000316_GR.
KEGGpol:Bpro_1784.
NMPDRfig|296591.1.peg.2822.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ12CM2.
OMAQ12CM2. FGPNEYH.

Enzyme and pathway databases

BioCycPSP296591:BPRO_1784-MON.

Family and domain databases

HAMAPMF_01601.
[Tree]
InterProIPR001509. Epimerase_deHydtase.
IPR011912. Heptose_epim.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHERPTHR10366:SF29. Heptose_epim. 1 hit.
PfamPF01370. Epimerase. 1 hit.
[Graphical view]
TIGRFAMsTIGR02197. heptose_epim. 1 hit.
ProtoNetSearch...

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Entry information

Entry nameHLDD_POLSJ
AccessionPrimary (citable) accession number: Q12CM2
Entry history
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: August 22, 2006
Last modified: June 16, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents