Reviewed, UniProtKB/Swiss-Prot Q12C42 (HEM6_POLSJ)

Names and origin

Protein names

Recommended name:
    Coproporphyrinogen-III oxidase, aerobic
      Short name=Coproporphyrinogenase
      Short name=Coprogen oxidase
    EC=1.3.3.3

Gene names

Name:	hemF
Ordered Locus Names:	Bpro_1970

Organism

Polaromonas sp. (strain JS666 / ATCC BAA-500) [Complete proteome] [HAMAP]

Taxonomic identifier

296591 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Polaromonas

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III By similarity. HAMAP MF_00333
Catalytic activity	Coproporphyrinogen-III + O2 + 2 H+ = protoporphyrinogen-IX + 2 CO2 + 2 H2O. HAMAP MF_00333
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2 route): step 1/1. HAMAP MF_00333
Subunit structure	Homodimer By similarity. HAMAP MF_00333
Subcellular location	Cytoplasm By similarity HAMAP MF_00333.
Sequence similarities	Belongs to the aerobic coproporphyrinogen-III oxidase family.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW porphyrin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	coproporphyrinogen oxidase activity Inferred from electronic annotation. Source: HAMAP protein homodimerization activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	Coproporphyrinogen-III oxidase, aerobic HAMAP MF_00333		PRO_1000019475
Regions
	Region	53 – 62	10	Important for dimerization By similarity
	Region	113 – 115	3	Substrate binding By similarity
	Region	248 – 283	36	Important for dimerization By similarity
	Region	266 – 271	6	Substrate binding By similarity
Sites
	Active site	111	1	Proton donor By similarity
	Binding site	97	1	Substrate By similarity
	Site	183	1	Important for dimerization By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q12C42-1 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: F3E91D4F08C07A34	FASTA	308	34,848
	10 20 30 40 50 60 MSMTELSGVR TFLLGLQERV TAAVAEVDGG DFITDAWQKE PGEPLQGNGI TKILENGEVF 70 80 90 100 110 120 ERAGCGFSHV QGSRLPPSAT QHRPELAGAP FEAMGVSLVF HPRNPYVPTV HMNVRMLAAT 130 140 150 160 170 180 PVSAGPAEAV CWFGGGMDLT PYYGFDEDAV HFHQTCKDAL APFGGDKYPR FKQWCDEYFY 190 200 210 220 230 240 LKHRQEQRGI GGIFFDDFQE LGLEQSFAMM QSVADSFLPA YLPIVRRRQS QPYGARERDF 250 260 270 280 290 300 QLYRRGRYVE FNLVWDRGTH FGLQSGGRTE SILMSMPPLA SWSYQHRAEA GSPEERLYKE FLIRRDWV

References

[1]

"Complete sequence of chromosome of Polaromonas sp. JS666." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Munk A.C., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Anderson I., Richardson P. Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000316 Genomic DNA. Translation: ABE43900.1.
RefSeq	YP_548798.1.
3D structure databases
SMR	Q12C42. Positions 5-308.
ModBase	Search...
Protein-protein interaction databases
STRING	Q12C42.
Genome annotation databases
GeneID	4015376.
GenomeReviews	Gene locus Bpro_1970 in contig CP000316_GR.
KEGG	pol:Bpro_1970.
NMPDR	fig|296591.1.peg.2636.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0408.
HOGENOM	HBG631180.
OMA	HMNTRFV.
PhylomeDB	Q12C42.
Enzyme and pathway databases
BioCyc	PSP296591:BPRO_1970-MONOMER.
Family and domain databases
HAMAP	MF_00333. Coprogen_oxidas. [Tree]
InterPro	IPR001260. Coprogen_oxidas. IPR018375. Coproporphyrinogen-3_ox_CS. [Graphical view]
Gene3D	G3DSA:3.40.1500.10. Coprogen_oxidas. 1 hit.
PANTHER	PTHR10755. Coprogen_oxidas. 1 hit.
Pfam	PF01218. Coprogen_oxidas. 1 hit. [Graphical view]
PIRSF	PIRSF000166. Coproporphyri_ox. 1 hit.
PRINTS	PR00073. COPRGNOXDASE.
PROSITE	PS01021. COPROGEN_OXIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM6_POLSJ

Accession

Primary (citable) accession number: Q12C42

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 22, 2006
Last modified:	January 19, 2010
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)