Q12A41 (LPXD_POLSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-3-O-acylglucosamine N-acyltransferase
EC=2.3.1.-

Gene names

Name:	lpxD
Ordered Locus Names:	Bpro_2685

Organism

Polaromonas sp. (strain JS666 / ATCC BAA-500) [Complete proteome] [HAMAP]

Taxonomic identifier

296591 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Polaromonas

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP MF_00523
Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-3-O-acyl-alpha-D-glucosamine = UDP-2,3-diacyl-alpha-D-glucosamine + [acyl-carrier-protein]. HAMAP MF_00523
Pathway	Bacterial outer membrane biogenesis; LPS lipid A biosynthesis. HAMAP MF_00523
Subunit structure	Homotrimer By similarity. HAMAP MF_00523
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxD subfamily.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring acyl groups other than amino-acyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 351	351	UDP-3-O-acylglucosamine N-acyltransferase HAMAP MF_00523		PRO_0000264405
Sites
	Active site	246	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q12A41 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 7BAD3E6787D55A98	FASTA	351	36,658
	10 20 30 40 50 60 MTLRLADIVQ SLAPTVQAEL VGNPDVQIER LSTLDAAGPH DLSFLSHPKY LAKLAQSAAA 70 80 90 100 110 120 CVVVAPAARE AAVSRGACIV VDDPYHYFAL ITQLWKYRHV LQAAPHIHPS AFIDPAATLA 130 140 150 160 170 180 PGVSVGAFAC ISAGTVIGAG ARIAEHCVIG RDAHVGAESR LSARVTVADG CYIGERCIVH 190 200 210 220 230 240 PGAVIGADGF GFAPHQGQWI KIEQLGAVKI GNDVEIGANT CIDRGALQDT VLEDGVKLDN 250 260 270 280 290 300 LVQIGHNVRI GKHTAMAGCA GVAGSATIGA HCTVGGGAIV LGHLSLADHV HISAASVVTR 310 320 330 340 350 SILKPGHYTG LFPIDDNTAW EKNAATLKQL HVLRERLKQA EKSLSNIQEK S

References

[1]

"Complete sequence of chromosome of Polaromonas sp. JS666." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Munk A.C., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Anderson I., Richardson P. Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JS666 / ATCC BAA-500.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000316 Genomic DNA. Translation: ABE44601.1.
RefSeq	YP_549499.1. NC_007948.1.
3D structure databases
ProteinModelPortal	Q12A41.
ModBase	Search...
Protein-protein interaction databases
STRING	Q12A41.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4014640.
GenomeReviews	Gene locus Bpro_2685 in contig CP000316_GR.
KEGG	pol:Bpro_2685.
NMPDR	fig|296591.1.peg.403.
PATRIC	22958516. VBIPolSp102244_2720.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1044.
HOGENOM	HBG469615.
OMA	CFVGKNT.
PhylomeDB	Q12A41.
ProtClustDB	PRK00892.
Enzyme and pathway databases
BioCyc	PSP296591:BPRO_2685-MONOMER.
Family and domain databases
HAMAP	MF_00523. LpxD. [Tree]
InterPro	IPR001451. Hexapep_transf. IPR018357. Hexapep_transf_CS. IPR011004. Trimer_LpxA-like. IPR007691. UDP-3-O_GlcNAc_AcTrfase. IPR020573. UDP_GlcNAc_AcTrfase_non-rep. [Graphical view]
KO	K02536.
Pfam	PF00132. Hexapep. 5 hits. PF04613. LpxD. 1 hit. [Graphical view]
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR01853. Lipid_A_lpxD. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPXD_POLSJ

Accession

Primary (citable) accession number: Q12A41

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	August 22, 2006
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program