ID   TSN31_HUMAN             Reviewed;         210 AA.
AC   Q12999; O00577; Q53X76;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   24-JAN-2024, entry version 160.
DE   RecName: Full=Tetraspanin-31;
DE            Short=Tspan-31;
DE   AltName: Full=Sarcoma-amplified sequence;
GN   Name=TSPAN31; Synonyms=SAS;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Osteosarcoma;
RX   PubMed=8134123;
RA   Jankowski S.A., Mitchell D.S., Smith S.H., Trent J.M., Meltzer P.S.;
RT   "SAS, a gene amplified in human sarcomas, encodes a new member of the
RT   transmembrane 4 superfamily of proteins.";
RL   Oncogene 9:1205-1211(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=9192850; DOI=10.1006/geno.1997.4727;
RA   Elkahloun A.G., Krizman D.B., Wang Z., Hofmann T.A., Roe B.A.,
RA   Meltzer P.S.;
RT   "Transcript mapping in a 46-kb sequenced region at the core of 12q13.3
RT   amplification in human cancers.";
RL   Genomics 42:295-301(1997).
RN   [3]
RP   SEQUENCE REVISION TO 76-77 AND 205-210.
RA   Roe B.A.;
RL   Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Trachea;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT   "Cloning of human full open reading frames in Gateway(TM) system entry
RT   vector (pDONR201).";
RL   Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [7]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Prostate;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- INTERACTION:
CC       Q12999; O95393: BMP10; NbExp=3; IntAct=EBI-17678331, EBI-3922513;
CC       Q12999; Q96DZ9-2: CMTM5; NbExp=3; IntAct=EBI-17678331, EBI-11522780;
CC       Q12999; P78329: CYP4F2; NbExp=3; IntAct=EBI-17678331, EBI-1752413;
CC       Q12999; Q9UHE5: NAT8; NbExp=3; IntAct=EBI-17678331, EBI-2863634;
CC       Q12999; Q9BRI3: SLC30A2; NbExp=3; IntAct=EBI-17678331, EBI-8644112;
CC       Q12999; O60636: TSPAN2; NbExp=3; IntAct=EBI-17678331, EBI-3914288;
CC   -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC   -!- SIMILARITY: Belongs to the tetraspanin (TM4SF) family. {ECO:0000305}.
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DR   EMBL; U01160; AAA17782.1; -; mRNA.
DR   EMBL; U81031; AAC39524.2; -; Genomic_DNA.
DR   EMBL; AK315765; BAG38117.1; -; mRNA.
DR   EMBL; CR407647; CAG28575.1; -; mRNA.
DR   EMBL; CH471054; EAW97057.1; -; Genomic_DNA.
DR   EMBL; BC010377; AAH10377.1; -; mRNA.
DR   CCDS; CCDS8952.1; -.
DR   PIR; I58391; I58391.
DR   RefSeq; NP_001317097.1; NM_001330168.1.
DR   RefSeq; NP_001317098.1; NM_001330169.1.
DR   RefSeq; NP_005972.1; NM_005981.4.
DR   AlphaFoldDB; Q12999; -.
DR   SMR; Q12999; -.
DR   BioGRID; 112209; 60.
DR   IntAct; Q12999; 6.
DR   STRING; 9606.ENSP00000257910; -.
DR   GlyCosmos; Q12999; 4 sites, No reported glycans.
DR   GlyGen; Q12999; 4 sites.
DR   iPTMnet; Q12999; -.
DR   PhosphoSitePlus; Q12999; -.
DR   SwissPalm; Q12999; -.
DR   BioMuta; TSPAN31; -.
DR   DMDM; 6174973; -.
DR   EPD; Q12999; -.
DR   jPOST; Q12999; -.
DR   MassIVE; Q12999; -.
DR   PaxDb; 9606-ENSP00000257910; -.
DR   PeptideAtlas; Q12999; -.
DR   ProteomicsDB; 59088; -.
DR   Pumba; Q12999; -.
DR   Antibodypedia; 28904; 143 antibodies from 26 providers.
DR   DNASU; 6302; -.
DR   Ensembl; ENST00000257910.8; ENSP00000257910.3; ENSG00000135452.10.
DR   GeneID; 6302; -.
DR   KEGG; hsa:6302; -.
DR   MANE-Select; ENST00000257910.8; ENSP00000257910.3; NM_005981.5; NP_005972.1.
DR   UCSC; uc001spt.4; human.
DR   AGR; HGNC:10539; -.
DR   CTD; 6302; -.
DR   DisGeNET; 6302; -.
DR   GeneCards; TSPAN31; -.
DR   HGNC; HGNC:10539; TSPAN31.
DR   HPA; ENSG00000135452; Low tissue specificity.
DR   MIM; 181035; gene.
DR   neXtProt; NX_Q12999; -.
DR   OpenTargets; ENSG00000135452; -.
DR   PharmGKB; PA34949; -.
DR   VEuPathDB; HostDB:ENSG00000135452; -.
DR   eggNOG; KOG3882; Eukaryota.
DR   GeneTree; ENSGT00940000160351; -.
DR   HOGENOM; CLU_088363_0_0_1; -.
DR   InParanoid; Q12999; -.
DR   OMA; IGAVYHH; -.
DR   OrthoDB; 4217282at2759; -.
DR   PhylomeDB; Q12999; -.
DR   TreeFam; TF323367; -.
DR   PathwayCommons; Q12999; -.
DR   SignaLink; Q12999; -.
DR   BioGRID-ORCS; 6302; 37 hits in 1157 CRISPR screens.
DR   ChiTaRS; TSPAN31; human.
DR   GeneWiki; TSPAN31; -.
DR   GenomeRNAi; 6302; -.
DR   Pharos; Q12999; Tbio.
DR   PRO; PR:Q12999; -.
DR   Proteomes; UP000005640; Chromosome 12.
DR   RNAct; Q12999; Protein.
DR   Bgee; ENSG00000135452; Expressed in pancreatic ductal cell and 207 other cell types or tissues.
DR   ExpressionAtlas; Q12999; baseline and differential.
DR   GO; GO:0016020; C:membrane; TAS:ProtInc.
DR   GO; GO:0005886; C:plasma membrane; TAS:ProtInc.
DR   GO; GO:0008284; P:positive regulation of cell population proliferation; TAS:ProtInc.
DR   InterPro; IPR018499; Tetraspanin/Peripherin.
DR   InterPro; IPR000301; Tetraspanin_animals.
DR   PANTHER; PTHR19282; TETRASPANIN; 1.
DR   PANTHER; PTHR19282:SF3; TETRASPANIN-31; 1.
DR   Pfam; PF00335; Tetraspanin; 1.
DR   PIRSF; PIRSF002419; Tetraspanin; 1.
DR   PRINTS; PR00259; TMFOUR.
DR   Genevisible; Q12999; HS.
PE   1: Evidence at protein level;
KW   Glycoprotein; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..210
FT                   /note="Tetraspanin-31"
FT                   /id="PRO_0000219270"
FT   TOPO_DOM        1..12
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        13..33
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        34..44
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        45..65
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        66..72
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        73..93
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        94..173
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        174..194
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        195..210
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        100
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        109
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        117
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        134
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   210 AA;  23053 MW;  8A1FF8CC5319755E CRC64;
     MVCGGFACSK NALCALNVVY MLVSLLLIGV AAWGKGLGLV SSIHIIGGVI AVGVFLLLIA
     VAGLVGAVNH HQVLLFFYMI ILGLVFIFQF VISCSCLAIN RSKQTDVINA SWWVMSNKTR
     DELERSFDCC GLFNLTTLYQ QDYDFCTAIC KSQSPTCQMC GEKFLKHSDE ALKILGGVGL
     FFSFTEILGV WLAMRFRNQK DPRANPSAFL
//