Q12986 (NFX1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcriptional repressor NF-X1
EC=6.3.2.-

Alternative name(s):
Nuclear transcription factor, X box-binding protein 1

Gene names

Name:	NFX1
Synonyms:	NFX2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1120 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds to the X-box motif of MHC class II genes and represses their expression. May play an important role in regulating the duration of an inflammatory response by limiting the period in which MHC class II molecules are induced by interferon-gamma. Isoform 3 binds to the X-box motif of TERT promoter and represses its expression. Together with PABPC1 or PABPC4, isoform 1 acts as a coactivator for TERT expression. Mediates E2-dependent ubiquitination. Ref.7 Ref.8 Ref.9
Subunit structure	Isoform 1 and isoform 3 interact with human papillomavirus (HPV) type-16 E6 oncoprotein. Isoform 1 interacts with PABPC1 and PABPC4. Ref.8 Ref.9
Subcellular location	Nucleus.
Induction	By IFNG/IFN-gamma.
Domain	The RING-type zinc finger domain interacts with an ubiquitin-conjugating enzyme (E2) and facilitates ubiquitination.
Post-translational modification	Isoform 3 is polyubiquitinated in the presence of HPV16 E6 protein; which leads to proteasomal degradation. Isoform 1 is not polyubiquitinated.
Sequence similarities	Belongs to the NFX1 family. Contains 8 NF-X1-type zinc fingers. Contains 1 R3H domain. Contains 1 RING-type zinc finger.
Sequence caution	The sequence AAA69517.1 differs from that shown. Reason: Frameshift at positions 78, 202 and 284.

Ontologies

Keywords
Biological process	Host-virus interaction Transcription Transcription regulation Ubl conjugation pathway
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Activator Ligase Repressor
PTM	Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	inflammatory response Traceable author statement Ref.6. Source: ProtInc negative regulation of MHC class II biosynthetic process Inferred from electronic annotation. Source: Compara negative regulation of transcription from RNA polymerase II promoter Traceable author statement Ref.6. Source: ProtInc transcription from RNA polymerase II promoter Traceable author statement Ref.6. Source: ProtInc virus-host interaction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW ligase activity Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Traceable author statement Ref.6. Source: ProtInc zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q12986-1)

Also known as: NFX-123;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q12986-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1014-1024: GKNSKKSHSFP → VEVETSHWTFL
     1025-1120: Missing.

Isoform 3 (identifier: Q12986-3)

Also known as: NFX-91;

The sequence of this isoform differs from the canonical sequence as follows:
     809-833: FRSNIPCHLVDISCGLPCSATLPCG → QSHYWASTQKKRSHYMKKIPAHACL
     834-1120: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1120	1120	Transcriptional repressor NF-X1		PRO_0000055979
Regions
	Domain	994 – 1062	69	R3H
	Zinc finger	358 – 409	52	RING-type; atypical
	Zinc finger	453 – 471	19	NF-X1-type 1
	Zinc finger	506 – 525	20	NF-X1-type 2
	Zinc finger	567 – 586	20	NF-X1-type 3
	Zinc finger	632 – 655	24	NF-X1-type 4
	Zinc finger	694 – 713	20	NF-X1-type 5
	Zinc finger	721 – 740	20	NF-X1-type 6
	Zinc finger	832 – 854	23	NF-X1-type 7
	Zinc finger	863 – 884	22	NF-X1-type 8
	Region	9 – 26	18	Interaction with PABPC1 and PABC4
	Compositional bias	1084 – 1089	6	Poly-Pro
Amino acid modifications
	Modified residue	50	1	Phosphoserine Ref.11
Natural variations
	Alternative sequence	809 – 833	25	FRSNI…TLPCG → QSHYWASTQKKRSHYMKKIP AHACL in isoform 3.		VSP_033682
	Alternative sequence	834 – 1120	287	Missing in isoform 3.		VSP_033683
	Alternative sequence	1014 – 1024	11	GKNSKKSHSFP → VEVETSHWTFL in isoform 2.		VSP_033684
	Alternative sequence	1025 – 1120	96	Missing in isoform 2.		VSP_033685
	Natural variant	731	1	H → Y. Corresponds to variant rs5017299 [ dbSNP | Ensembl ].		VAR_043380
	Natural variant	760	1	P → S. Corresponds to variant rs2860036 [ dbSNP | Ensembl ].		VAR_043381
	Natural variant	1086	1	P → Q. Corresponds to variant rs2274866 [ dbSNP | Ensembl ].		VAR_043382
Experimental info
	Mutagenesis	20	1	F → A: Reduces PABPC1 and PABC4 binding. Ref.9
	Sequence conflict	179	1	G → A in AAA69517. Ref.1
	Sequence conflict	186	1	G → E in AAA69517. Ref.1
	Sequence conflict	312	1	Q → P in AAA69517. Ref.1
	Sequence conflict	419 – 420	2	YT → FS in AAA69517. Ref.1
	Sequence conflict	560	1	I → T in AAA69517. Ref.1
	Sequence conflict	653	1	C → V in AAA69517. Ref.1
	Sequence conflict	678	1	E → EA in BAF84332. Ref.2
	Sequence conflict	714	1	I → N in AAA69517. Ref.1
	Sequence conflict	786	1	C → G in AAA69517. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (NFX-123) [UniParc]. Last modified May 20, 2008. Version 2. Checksum: F2203BE1DB6437E6	FASTA	1,120	124,395
	10 20 30 40 50 60 MAEAPPVSGT FKFNTDAAEF IPQEKKNSGL NCGTQRRLDS NRIGRRNYSS PPPCHLSRQV 70 80 90 100 110 120 PYDEISAVHQ HSYHPSGSKP KSQQTSFQSS PCNKSPKSHG LQNQPWQKLR NEKHHIRVKK 130 140 150 160 170 180 AQSLAEQTSD TAGLESSTRS ESGTDLREHS PSESEKEVVG ADPRGAKPKK ATQFVYSYGR 190 200 210 220 230 240 GPKVKGKLKC EWSNRTTPKP EDAGPESTKP VGVFHPDSSE ASSRKGVLDG YGARRNEQRR 250 260 270 280 290 300 YPQKRPPWEV EGARPRPGRN PPKQEGHRHT NAGHRNNMGP IPKDDLNERP AKSTCDSENL 310 320 330 340 350 360 AVINKSSRRV DQEKCTVRRQ DPQVVSPFSR GKQNHVLKNV ETHTGSLIEQ LTTEKYECMV 370 380 390 400 410 420 CCELVRVTAP VWSCQSCYHV FHLNCIKKWA RSPASQADGQ SGWRCPACQN VSAHVPNTYT 430 440 450 460 470 480 CFCGKVKNPE WSRNEIPHSC GEVCRKKQPG QDCPHSCNLL CHPGPCPPCP AFMTKTCECG 490 500 510 520 530 540 RTRHTVRCGQ AVSVHCSNPC ENILNCGQHQ CAELCHGGQC QPCQIILNQV CYCGSTSRDV 550 560 570 580 590 600 LCGTDVGKSD GFGDFSCLKI CGKDLKCGNH TCSQVCHPQP CQQCPRLPQL VRCCPCGQTP 610 620 630 640 650 660 LSQLLELGSS SRKTCMDPVP SCGKVCGKPL PCGSLDFIHT CEKLCHEGDC GPCSRTSVIS 670 680 690 700 710 720 CRCSFRTKEL PCTSLKSEDA TFMCDKRCNK KRLCGRHKCN EICCVDKEHK CPLICGRKLR 730 740 750 760 770 780 CGLHRCEEPC HRGNCQTCWQ ASFDELTCHC GASVIYPPVP CGTRPPECTQ TCARVHECDH 790 800 810 820 830 840 PVYHSCHSEE KCPPCTFLTQ KWCMGKHEFR SNIPCHLVDI SCGLPCSATL PCGMHKCQRL 850 860 870 880 890 900 CHKGECLVDE PCKQPCTTPR ADCGHPCMAP CHTSSPCPVT ACKAKVELQC ECGRRKEMVI 910 920 930 940 950 960 CSEASSTYQR IAAISMASKI TDMQLGGSVE ISKLITKKEV HQARLECDEE CSALERKKRL 970 980 990 1000 1010 1020 AEAFHISEDS DPFNIRSSGS KFSDSLKEDA RKDLKFVSDV EKEMETLVEA VNKGKNSKKS 1030 1040 1050 1060 1070 1080 HSFPPMNRDH RRIIHDLAQV YGLESVSYDS EPKRNVVVTA IRGKSVCPPT TLTGVLEREM 1090 1100 1110 1120 QARPPPPIPH HRHQSDKNPG SSNLQKITKE PIIDYFDVQD
	Isoform 2 [UniParc]. Checksum: 4228E14EEF42E3BC	FASTA	1,024	113,623
	10 20 30 40 50 60 MAEAPPVSGT FKFNTDAAEF IPQEKKNSGL NCGTQRRLDS NRIGRRNYSS PPPCHLSRQV 70 80 90 100 110 120 PYDEISAVHQ HSYHPSGSKP KSQQTSFQSS PCNKSPKSHG LQNQPWQKLR NEKHHIRVKK 130 140 150 160 170 180 AQSLAEQTSD TAGLESSTRS ESGTDLREHS PSESEKEVVG ADPRGAKPKK ATQFVYSYGR 190 200 210 220 230 240 GPKVKGKLKC EWSNRTTPKP EDAGPESTKP VGVFHPDSSE ASSRKGVLDG YGARRNEQRR 250 260 270 280 290 300 YPQKRPPWEV EGARPRPGRN PPKQEGHRHT NAGHRNNMGP IPKDDLNERP AKSTCDSENL 310 320 330 340 350 360 AVINKSSRRV DQEKCTVRRQ DPQVVSPFSR GKQNHVLKNV ETHTGSLIEQ LTTEKYECMV 370 380 390 400 410 420 CCELVRVTAP VWSCQSCYHV FHLNCIKKWA RSPASQADGQ SGWRCPACQN VSAHVPNTYT 430 440 450 460 470 480 CFCGKVKNPE WSRNEIPHSC GEVCRKKQPG QDCPHSCNLL CHPGPCPPCP AFMTKTCECG 490 500 510 520 530 540 RTRHTVRCGQ AVSVHCSNPC ENILNCGQHQ CAELCHGGQC QPCQIILNQV CYCGSTSRDV 550 560 570 580 590 600 LCGTDVGKSD GFGDFSCLKI CGKDLKCGNH TCSQVCHPQP CQQCPRLPQL VRCCPCGQTP 610 620 630 640 650 660 LSQLLELGSS SRKTCMDPVP SCGKVCGKPL PCGSLDFIHT CEKLCHEGDC GPCSRTSVIS 670 680 690 700 710 720 CRCSFRTKEL PCTSLKSEDA TFMCDKRCNK KRLCGRHKCN EICCVDKEHK CPLICGRKLR 730 740 750 760 770 780 CGLHRCEEPC HRGNCQTCWQ ASFDELTCHC GASVIYPPVP CGTRPPECTQ TCARVHECDH 790 800 810 820 830 840 PVYHSCHSEE KCPPCTFLTQ KWCMGKHEFR SNIPCHLVDI SCGLPCSATL PCGMHKCQRL 850 860 870 880 890 900 CHKGECLVDE PCKQPCTTPR ADCGHPCMAP CHTSSPCPVT ACKAKVELQC ECGRRKEMVI 910 920 930 940 950 960 CSEASSTYQR IAAISMASKI TDMQLGGSVE ISKLITKKEV HQARLECDEE CSALERKKRL 970 980 990 1000 1010 1020 AEAFHISEDS DPFNIRSSGS KFSDSLKEDA RKDLKFVSDV EKEMETLVEA VNKVEVETSH WTFL
	Isoform 3 (NFX-91) [UniParc]. Checksum: AD2A1E26911EA705	FASTA	833	92,679
	10 20 30 40 50 60 MAEAPPVSGT FKFNTDAAEF IPQEKKNSGL NCGTQRRLDS NRIGRRNYSS PPPCHLSRQV 70 80 90 100 110 120 PYDEISAVHQ HSYHPSGSKP KSQQTSFQSS PCNKSPKSHG LQNQPWQKLR NEKHHIRVKK 130 140 150 160 170 180 AQSLAEQTSD TAGLESSTRS ESGTDLREHS PSESEKEVVG ADPRGAKPKK ATQFVYSYGR 190 200 210 220 230 240 GPKVKGKLKC EWSNRTTPKP EDAGPESTKP VGVFHPDSSE ASSRKGVLDG YGARRNEQRR 250 260 270 280 290 300 YPQKRPPWEV EGARPRPGRN PPKQEGHRHT NAGHRNNMGP IPKDDLNERP AKSTCDSENL 310 320 330 340 350 360 AVINKSSRRV DQEKCTVRRQ DPQVVSPFSR GKQNHVLKNV ETHTGSLIEQ LTTEKYECMV 370 380 390 400 410 420 CCELVRVTAP VWSCQSCYHV FHLNCIKKWA RSPASQADGQ SGWRCPACQN VSAHVPNTYT 430 440 450 460 470 480 CFCGKVKNPE WSRNEIPHSC GEVCRKKQPG QDCPHSCNLL CHPGPCPPCP AFMTKTCECG 490 500 510 520 530 540 RTRHTVRCGQ AVSVHCSNPC ENILNCGQHQ CAELCHGGQC QPCQIILNQV CYCGSTSRDV 550 560 570 580 590 600 LCGTDVGKSD GFGDFSCLKI CGKDLKCGNH TCSQVCHPQP CQQCPRLPQL VRCCPCGQTP 610 620 630 640 650 660 LSQLLELGSS SRKTCMDPVP SCGKVCGKPL PCGSLDFIHT CEKLCHEGDC GPCSRTSVIS 670 680 690 700 710 720 CRCSFRTKEL PCTSLKSEDA TFMCDKRCNK KRLCGRHKCN EICCVDKEHK CPLICGRKLR 730 740 750 760 770 780 CGLHRCEEPC HRGNCQTCWQ ASFDELTCHC GASVIYPPVP CGTRPPECTQ TCARVHECDH 790 800 810 820 830 PVYHSCHSEE KCPPCTFLTQ KWCMGKHEQS HYWASTQKKR SHYMKKIPAH ACL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Li J.M., Sah J.H., Zhou Z.M. Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Testis.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Placenta.
[3]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Eye.
[6]	"A novel cysteine-rich sequence-specific DNA-binding protein interacts with the conserved X-box motif of the human major histocompatibility complex class II genes via a repeated Cys-His domain and functions as a transcriptional repressor." Song Z., Krishna S., Thanos D., Strominger J.L., Ono S.J. J. Exp. Med. 180:1763-1774(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 48-1120 (ISOFORM 1).
[7]	"RING fingers mediate ubiquitin-conjugating enzyme (E2)-dependent ubiquitination." Lorick K.L., Jensen J.P., Fang S., Ong A.M., Hatakeyama S., Weissman A.M. Proc. Natl. Acad. Sci. U.S.A. 96:11364-11369(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS AN E2-DEPENDENT UBIQUITIN-PROTEIN LIGASE.
[8]	"Identification of a novel telomerase repressor that interacts with the human papillomavirus type-16 E6/E6-AP complex." Gewin L., Myers H., Kiyono T., Galloway D.A. Genes Dev. 18:2269-2282(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH HPV16 E6, FUNCTION.
[9]	"NFX1-123 and poly(A) binding proteins synergistically augment activation of telomerase in human papillomavirus type 16 E6-expressing cells." Katzenellenbogen R.A., Egelkrout E.M., Vliet-Gregg P., Gewin L.C., Gafken P.R., Galloway D.A. J. Virol. 81:3786-3796(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH PABPC1 AND PABPC4, MUTAGENESIS OF PHE-20.
[10]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[11]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-50, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[12]	"Large-scale proteomics analysis of the human kinome." Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H. Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF332009 mRNA. Translation: AAK16545.1. AK291643 mRNA. Translation: BAF84332.1. AL356472, AL356218 Genomic DNA. Translation: CAH72745.1. AL356472, AL356218 Genomic DNA. Translation: CAH72746.1. AL356472 Genomic DNA. Translation: CAH72747.3. AL356218, AL356472 Genomic DNA. Translation: CAI13304.1. AL356218, AL356472 Genomic DNA. Translation: CAI13305.1. CH471071 Genomic DNA. Translation: EAW58510.1. CH471071 Genomic DNA. Translation: EAW58511.1. BC012151 mRNA. Translation: AAH12151.1. U15306 mRNA. Translation: AAA69517.1. Frameshift.
IPI	IPI00158157. IPI00158159. IPI00843917.
PIR	I38869.
RefSeq	NP_002495.2. NM_002504.4. NP_667344.1. NM_147133.2. NP_667345.1. NM_147134.2.
UniGene	Hs.413074.
3D structure databases
ProteinModelPortal	Q12986.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q12986. 2 interactions.
STRING	9606.ENSP00000368856.
PTM databases
PhosphoSite	Q12986.
Polymorphism databases
DMDM	189047116.
Proteomic databases
PaxDb	Q12986.
PRIDE	Q12986.
Protocols and materials databases
DNASU	4799.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000318524; ENSP00000317695; ENSG00000086102. ENST00000379521; ENSP00000368836; ENSG00000086102. ENST00000379540; ENSP00000368856; ENSG00000086102.
GeneID	4799.
KEGG	hsa:4799.
UCSC	uc003zso.3. human. uc003zsp.2. human. uc003zsq.3. human.
Organism-specific databases
CTD	4799.
GeneCards	GC09P033280.
HGNC	HGNC:7803. NFX1.
MIM	603255. gene.
neXtProt	NX_Q12986.
PharmGKB	PA31608.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG12793.
HOGENOM	HOG000006942.
HOVERGEN	HBG003827.
InParanoid	Q12986.
KO	K12236.
OMA	LVRYCPC.
OrthoDB	EOG4KSPHZ.
PhylomeDB	Q12986.
Gene expression databases
Bgee	Q12986.
CleanEx	HS_NFX1.
Genevestigator	Q12986.
GermOnline	ENSG00000086102. Homo sapiens.
Family and domain databases
InterPro	IPR001374. R3H_ss-bd. IPR019786. Zinc_finger_PHD-type_CS. IPR000967. Znf_NFX1. IPR019787. Znf_PHD-finger. IPR001841. Znf_RING. [Graphical view]
Pfam	PF01424. R3H. 1 hit. PF01422. zf-NF-X1. 5 hits. [Graphical view]
SMART	SM00393. R3H. 1 hit. SM00184. RING. 1 hit. SM00438. ZnF_NFX. 9 hits. [Graphical view]
PROSITE	PS51061. R3H. 1 hit. PS00518. ZF_RING_1. False negative. PS50089. ZF_RING_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	NFX1. human.
GenomeRNAi	4799.
NextBio	18492.
SOURCE	Search...

Entry information

Entry name

NFX1_HUMAN

Accession

Primary (citable) accession number: Q12986
Secondary accession number(s): A8K6H8 , Q5VXW6, Q96EL5, Q9BXI1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 20, 2008
Last modified:	May 1, 2013
This is version 129 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.