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Protein

BCL2/adenovirus E1B 19 kDa protein-interacting protein 3

Gene

BNIP3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Apoptosis-inducing protein that can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates in mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. Plays an important role in the calprotectin (S100A8/A9)-induced cell death pathway.2 Publications

GO - Molecular functioni

  • GTPase binding Source: BHF-UCL
  • identical protein binding Source: IntAct
  • protein heterodimerization activity Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB

GO - Biological processi

  • apoptotic process Source: MGI
  • autophagic cell death Source: Ensembl
  • brown fat cell differentiation Source: Ensembl
  • cardiac muscle cell apoptotic process Source: Ensembl
  • cell death Source: UniProtKB
  • cellular response to cobalt ion Source: BHF-UCL
  • cellular response to hydrogen peroxide Source: Ensembl
  • cellular response to hypoxia Source: BHF-UCL
  • cellular response to mechanical stimulus Source: UniProtKB
  • cerebral cortex development Source: Ensembl
  • defense response to virus Source: UniProtKB
  • granzyme-mediated apoptotic signaling pathway Source: UniProtKB
  • intrinsic apoptotic signaling pathway in response to hypoxia Source: BHF-UCL
  • mitochondrial fragmentation involved in apoptotic process Source: BHF-UCL
  • mitochondrial outer membrane permeabilization Source: UniProtKB
  • mitochondrial protein catabolic process Source: UniProtKB
  • mitophagy Source: ParkinsonsUK-UCL
  • negative regulation of apoptotic process Source: UniProtKB
  • negative regulation of cell death Source: MGI
  • negative regulation of membrane potential Source: UniProtKB
  • negative regulation of mitochondrial fusion Source: BHF-UCL
  • negative regulation of mitochondrial membrane permeability involved in apoptotic process Source: Ensembl
  • negative regulation of mitochondrial membrane potential Source: Ensembl
  • negative regulation of reactive oxygen species metabolic process Source: Ensembl
  • neuron apoptotic process Source: UniProtKB
  • oligodendrocyte differentiation Source: Ensembl
  • positive regulation of apoptotic process Source: UniProtKB
  • positive regulation of autophagy Source: UniProtKB
  • positive regulation of cardiac muscle cell apoptotic process Source: Ensembl
  • positive regulation of macroautophagy Source: MGI
  • positive regulation of mitochondrial calcium ion concentration Source: Ensembl
  • positive regulation of mitochondrial fission Source: BHF-UCL
  • positive regulation of mitophagy Source: Ensembl
  • positive regulation of necrotic cell death Source: Ensembl
  • positive regulation of programmed cell death Source: UniProtKB
  • positive regulation of protein complex disassembly Source: BHF-UCL
  • positive regulation of release of cytochrome c from mitochondria Source: BHF-UCL
  • reactive oxygen species metabolic process Source: UniProtKB
  • regulation of aerobic respiration Source: Ensembl
  • regulation of mitochondrial membrane permeability Source: UniProtKB
  • response to hyperoxia Source: Ensembl
  • response to hypoxia Source: UniProtKB
  • toxin transport Source: Ensembl

Keywordsi

Biological processApoptosis, Host-virus interaction

Enzyme and pathway databases

SIGNORiQ12983.

Protein family/group databases

TCDBi1.A.20.1.1. the bcl2/adenovirus e1b-interacting protein 3 (bnip3) family.

Names & Taxonomyi

Protein namesi
Recommended name:
BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
Gene namesi
Name:BNIP3
Synonyms:NIP3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:1084. BNIP3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei229 – 249HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • dendrite Source: UniProtKB
  • endoplasmic reticulum Source: GO_Central
  • integral component of mitochondrial outer membrane Source: UniProtKB
  • mitochondrial membrane Source: UniProtKB
  • mitochondrial outer membrane Source: UniProtKB
  • mitochondrion Source: UniProtKB
  • nuclear envelope Source: UniProtKB
  • nucleoplasm Source: UniProtKB
  • nucleus Source: UniProtKB
  • postsynaptic density Source: Ensembl

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi664.
PharmGKBiPA25394.

Polymorphism and mutation databases

BioMutaiBNIP3.
DMDMi20532402.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000649641 – 259BCL2/adenovirus E1B 19 kDa protein-interacting protein 3Add BLAST259

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei119PhosphoserineCombined sources1
Modified residuei131PhosphoserineCombined sources1
Modified residuei151PhosphoserineCombined sources1
Modified residuei157PhosphoserineBy similarity1
Modified residuei160PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ12983.
MaxQBiQ12983.
PaxDbiQ12983.
PeptideAtlasiQ12983.
PRIDEiQ12983.

PTM databases

iPTMnetiQ12983.
PhosphoSitePlusiQ12983.

Expressioni

Gene expression databases

BgeeiENSG00000176171.
CleanExiHS_BNIP3.
ExpressionAtlasiQ12983. baseline and differential.
GenevisibleiQ12983. HS.

Organism-specific databases

HPAiCAB011676.
HPA003015.

Interactioni

Subunit structurei

Homodimer. Binds to BCL2. Interacts with BNIP3L and ACAA2. Also can interact with adenovirus E1B 19 kDa protein or Epstein-Barr virus BHRF1. Interacts (via BH3 domain) with SPATA18 (via coiled-coil domains). Interacts with BOK; promotes BOK oligomerization (PubMed:15868100).5 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • GTPase binding Source: BHF-UCL
  • identical protein binding Source: IntAct
  • protein heterodimerization activity Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB

Protein-protein interaction databases

BioGridi107132. 23 interactors.
DIPiDIP-34429N.
IntActiQ12983. 31 interactors.
MINTiMINT-1475327.
STRINGi9606.ENSP00000357625.

Structurei

Secondary structure

1259
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi224 – 253Combined sources30

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2J5DNMR-A/B211-255[»]
2KA1NMR-A/B219-253[»]
2KA2NMR-A/B219-253[»]
ProteinModelPortaliQ12983.
SMRiQ12983.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ12983.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi165 – 190BH3Add BLAST26

Sequence similaritiesi

Belongs to the NIP3 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IHYW. Eukaryota.
ENOG4111KXP. LUCA.
HOVERGENiHBG050707.
InParanoidiQ12983.
KOiK15464.
OrthoDBiEOG091G0REJ.
PhylomeDBiQ12983.
TreeFamiTF315424.

Family and domain databases

InterProiView protein in InterPro
IPR010548. BNIP3.
PANTHERiPTHR15186. PTHR15186. 1 hit.
PfamiView protein in Pfam
PF06553. BNIP3. 1 hit.

Sequencei

Sequence statusi: Complete.

Q12983-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDAAADPPG PALPCEFLRP GCGAPLSPGA QLGRGAPTSA FPPPAAEAHP
60 70 80 90 100
AARRGLRSPQ LPSGAMSQNG APGMQEESLQ GSWVELHFSN NGNGGSVPAS
110 120 130 140 150
VSIYNGDMEK ILLDAQHESG RSSSKSSHCD SPPRSQTPQD TNRASETDTH
160 170 180 190 200
SIGEKNSSQS EEDDIERRKE VESILKKNSD WIWDWSSRPE NIPPKEFLFK
210 220 230 240 250
HPKRTATLSM RNTSVMKKGG IFSAEFLKVF LPSLLLSHLL AIGLGIYIGR

RLTTSTSTF
Length:259
Mass (Da):27,832
Last modified:June 7, 2017 - v3
Checksum:i53967696EDA005E2
GO

Sequence cautioni

The sequence AAC00022 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAC16738 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAH21989 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAW62256 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti68Q → E in AAC00022 (PubMed:7954800).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY886764 Genomic DNA. Translation: AAW62256.1. Different initiation.
AL162274 Genomic DNA. No translation available.
BC009342 mRNA. No translation available.
BC021989 mRNA. Translation: AAH21989.1. Different initiation.
AF002697 mRNA. Translation: AAC16738.1. Different initiation.
U15174 mRNA. Translation: AAC00022.1. Different initiation.
PIRiI38865.
RefSeqiNP_004043.3. NM_004052.3.
UniGeneiHs.144873.

Genome annotation databases

EnsembliENST00000368636; ENSP00000357625; ENSG00000176171.
GeneIDi664.
KEGGihsa:664.
UCSCiuc001lkv.2. human.

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology
NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY886764 Genomic DNA. Translation: AAW62256.1. Different initiation.
AL162274 Genomic DNA. No translation available.
BC009342 mRNA. No translation available.
BC021989 mRNA. Translation: AAH21989.1. Different initiation.
AF002697 mRNA. Translation: AAC16738.1. Different initiation.
U15174 mRNA. Translation: AAC00022.1. Different initiation.
PIRiI38865.
RefSeqiNP_004043.3. NM_004052.3.
UniGeneiHs.144873.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2J5DNMR-A/B211-255[»]
2KA1NMR-A/B219-253[»]
2KA2NMR-A/B219-253[»]
ProteinModelPortaliQ12983.
SMRiQ12983.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107132. 23 interactors.
DIPiDIP-34429N.
IntActiQ12983. 31 interactors.
MINTiMINT-1475327.
STRINGi9606.ENSP00000357625.

Protein family/group databases

TCDBi1.A.20.1.1. the bcl2/adenovirus e1b-interacting protein 3 (bnip3) family.

PTM databases

iPTMnetiQ12983.
PhosphoSitePlusiQ12983.

Polymorphism and mutation databases

BioMutaiBNIP3.
DMDMi20532402.

Proteomic databases

EPDiQ12983.
MaxQBiQ12983.
PaxDbiQ12983.
PeptideAtlasiQ12983.
PRIDEiQ12983.

Protocols and materials databases

DNASUi664.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000368636; ENSP00000357625; ENSG00000176171.
GeneIDi664.
KEGGihsa:664.
UCSCiuc001lkv.2. human.

Organism-specific databases

CTDi664.
DisGeNETi664.
GeneCardsiBNIP3.
H-InvDBiHIX0037770.
HGNCiHGNC:1084. BNIP3.
HPAiCAB011676.
HPA003015.
MIMi603293. gene.
neXtProtiNX_Q12983.
PharmGKBiPA25394.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IHYW. Eukaryota.
ENOG4111KXP. LUCA.
HOVERGENiHBG050707.
InParanoidiQ12983.
KOiK15464.
OrthoDBiEOG091G0REJ.
PhylomeDBiQ12983.
TreeFamiTF315424.

Enzyme and pathway databases

SIGNORiQ12983.

Miscellaneous databases

EvolutionaryTraceiQ12983.
GeneWikiiBNIP3.
GenomeRNAii664.
PROiPR:Q12983.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000176171.
CleanExiHS_BNIP3.
ExpressionAtlasiQ12983. baseline and differential.
GenevisibleiQ12983. HS.

Family and domain databases

InterProiView protein in InterPro
IPR010548. BNIP3.
PANTHERiPTHR15186. PTHR15186. 1 hit.
PfamiView protein in Pfam
PF06553. BNIP3. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBNIP3_HUMAN
AccessioniPrimary (citable) accession number: Q12983
Secondary accession number(s): O14620, Q96GP0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: June 7, 2017
Last modified: June 7, 2017
This is version 167 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.