Q12981 (SEC20_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vesicle transport protein SEC20

Alternative name(s):
BCL2/adenovirus E1B 19 kDa protein-interacting protein 1
Transformation-related gene 8 protein
Short name=TRG-8

Gene names

Name:	BNIP1
Synonyms:	NIP1, SEC20L
ORF Names:	TRG8

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	228 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	SNARE that may be involved in targeting and fusion of Golgi-derived retrograde transport vesicles with the ER. Required for maintenance of ER network. Implicated in the suppression of cell death. May be involved in mitochondrial autophagy. Ref.7 Ref.9
Subunit structure	Component of a SNARE complex consisting of STX18, USE1L, BNIP1/SEC20L and SEC22B. Interacts directly with STX18, RINT1/TIP20L and NAPA. Interacts with ZW10 through RINT1. Interacts with BCL2 and adenovirus E1B 19 kDa protein. Ref.7
Subcellular location	Mitochondrion. Endoplasmic reticulum membrane; Single-pass type IV membrane protein Ref.7 Ref.9.
Tissue specificity	Isoform 1 is highly expressed in heart, brain, liver skeletal muscle and pancreas. Isoform 3 is moderately expressed in placenta, lung and kidney. Isoform 4 is highly expressed in testis and small intestine. Ref.2
Post-translational modification	'Lys-63'-linked polyubiquitination by RNF185 allows recruiting of autophagy receptor SQSTM1, which simultaneously binds both ubiquitin and LC3 to link ubiquitination and autophagy.
Miscellaneous	Silencing of BNIP1 disrupts ER morphology.
Sequence similarities	Belongs to the SEC20 family.

Ontologies

Keywords
Biological process	Apoptosis Autophagy ER-Golgi transport Transport
Cellular component	Endoplasmic reticulum Membrane Mitochondrion
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil Transmembrane Transmembrane helix
PTM	Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from physical interaction Ref.1. Source: MGI autophagy Inferred from electronic annotation. Source: UniProtKB-KW endoplasmic reticulum membrane fusion Inferred from mutant phenotype Ref.7. Source: HGNC endoplasmic reticulum organization Inferred from mutant phenotype Ref.7. Source: HGNC induction of apoptosis Inferred from mutant phenotype Ref.7. Source: HGNC negative regulation of apoptotic process Traceable author statement Ref.1. Source: UniProtKB vesicle-mediated transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	SNARE complex Inferred from direct assay Ref.7. Source: HGNC integral to endoplasmic reticulum membrane Traceable author statement Ref.7. Source: HGNC mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell nuclear envelope Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: Q12981-4)

Also known as: BNIP1; S4;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q12981-2)

Also known as: BNIP1-a; S1;

The sequence of this isoform differs from the canonical sequence as follows:
     90-123: Missing.

Isoform 3 (identifier: Q12981-1)

Also known as: BNIP1-b; S2;

The sequence of this isoform differs from the canonical sequence as follows:
     59-59: Q → QPVLYQRAFIWTASTFFFKLTYSLTDFSSTQHDFNSPTTPVTFS

Isoform 4 (identifier: Q12981-3)

Also known as: BNIP1-c; S3;

The sequence of this isoform differs from the canonical sequence as follows:
     59-59: Q → QPVLYQRAFIWTASTFFFKLTYSLTDFSSTQHDFNSPTTPVTFS
     90-123: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 228	228	Vesicle transport protein SEC20		PRO_0000064960
Regions
	Topological domain	1 – 199	199	Cytoplasmic Potential
	Transmembrane	200 – 220	21	Helical; Anchor for type IV membrane protein; Potential
	Topological domain	221 – 228	8	Lumenal Potential
	Coiled coil	37 – 90	54	Potential
Natural variations
	Alternative sequence	59	1	Q → QPVLYQRAFIWTASTFFFKL TYSLTDFSSTQHDFNSPTTP VTFS in isoform 3 and isoform 4.		VSP_017901
	Alternative sequence	90 – 123	34	Missing in isoform 2 and isoform 4.		VSP_004330
	Natural variant	14	1	Q → H. Ref.4 Corresponds to variant rs5745100 [ dbSNP | Ensembl ].		VAR_019169
Experimental info
	Mutagenesis	114	1	L → A: Loss of proapoptotic effect. No effect on interaction with RINT1. Ref.7
	Sequence conflict	14	1	Q → R in AAO91805. Ref.3
	Sequence conflict	66	1	K → E in AAO91805. Ref.3
	Sequence conflict	210	1	A → R Ref.1
	Sequence conflict	210	1	A → R Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (BNIP1) (S4) [UniParc]. Last modified April 18, 2006. Version 3. Checksum: 23F4C21E7327DE3D	FASTA	228	26,132
	10 20 30 40 50 60 MAAPQDVHVR ICNQEIVKFD LEVKALIQDI RDCSGPLSAL TELNTKVKEK FQQLRHRIQD 70 80 90 100 110 120 LEQLAKEQDK ESEKQLLLQE VENHKKQMLS NQASWRKANL TCKIAIDNLE KAELLQGGDL 130 140 150 160 170 180 LRQRKTTKES LAQTSSTITE SLMGISRMMA QQVQQSEEAM QSLVTSSRTI LDANEEFKSM 190 200 210 220 SGTIQLGRKL ITKYNRRELT DKLLIFLALA LFLATVLYIV KKRLFPFL
	Isoform 2 (BNIP1-a) (S1) [UniParc]. Checksum: E1AAA43E09F6F7F4	FASTA	194	22,352
	10 20 30 40 50 60 MAAPQDVHVR ICNQEIVKFD LEVKALIQDI RDCSGPLSAL TELNTKVKEK FQQLRHRIQD 70 80 90 100 110 120 LEQLAKEQDK ESEKQLLLQE VENHKKQMLR KTTKESLAQT SSTITESLMG ISRMMAQQVQ 130 140 150 160 170 180 QSEEAMQSLV TSSRTILDAN EEFKSMSGTI QLGRKLITKY NRRELTDKLL IFLALALFLA 190 TVLYIVKKRL FPFL
	Isoform 3 (BNIP1-b) (S2) [UniParc]. Checksum: 2E5226F028FF2D1A	FASTA	271	31,113
	10 20 30 40 50 60 MAAPQDVHVR ICNQEIVKFD LEVKALIQDI RDCSGPLSAL TELNTKVKEK FQQLRHRIQP 70 80 90 100 110 120 VLYQRAFIWT ASTFFFKLTY SLTDFSSTQH DFNSPTTPVT FSDLEQLAKE QDKESEKQLL 130 140 150 160 170 180 LQEVENHKKQ MLSNQASWRK ANLTCKIAID NLEKAELLQG GDLLRQRKTT KESLAQTSST 190 200 210 220 230 240 ITESLMGISR MMAQQVQQSE EAMQSLVTSS RTILDANEEF KSMSGTIQLG RKLITKYNRR 250 260 270 ELTDKLLIFL ALALFLATVL YIVKKRLFPF L
	Isoform 4 (BNIP1-c) (S3) [UniParc]. Checksum: FDE67A8CE1CE1AAB	FASTA	237	27,333
	10 20 30 40 50 60 MAAPQDVHVR ICNQEIVKFD LEVKALIQDI RDCSGPLSAL TELNTKVKEK FQQLRHRIQP 70 80 90 100 110 120 VLYQRAFIWT ASTFFFKLTY SLTDFSSTQH DFNSPTTPVT FSDLEQLAKE QDKESEKQLL 130 140 150 160 170 180 LQEVENHKKQ MLRKTTKESL AQTSSTITES LMGISRMMAQ QVQQSEEAMQ SLVTSSRTIL 190 200 210 220 230 DANEEFKSMS GTIQLGRKLI TKYNRRELTD KLLIFLALAL FLATVLYIVK KRLFPFL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Adenovirus E1B 19 kDa and Bcl-2 proteins interact with a common set of cellular proteins." Boyd J.M., Malstrom S., Subramanian T., Venkatesh L.K., Schaeper U., Elangovan B., D'Sa-Eipper C., Chinnadurai G. Cell 79:341-351(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Novel BNIP1 variants and their interaction with BCL2 family members." Zhang H., Heim J., Meyhack B. FEBS Lett. 448:23-27(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2; 3 AND 4), TISSUE SPECIFICITY.
[3]	"Identification of human transformation-related gene." Kim J.W. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	NIEHS SNPs program Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT HIS-14.
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Kidney.
[7]	"Involvement of BNIP1 in apoptosis and endoplasmic reticulum membrane fusion." Nakajima K., Hirose H., Taniguchi M., Kurashina H., Arasaki K., Nagahama M., Tani K., Yamamoto A., Tagaya M. EMBO J. 23:3216-3226(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH STX18 AND RINT1, MUTAGENESIS OF LEU-114.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]	"RNF185, a novel mitochondrial ubiquitin E3 ligase, regulates autophagy through interaction with BNIP1." Tang F., Wang B., Li N., Wu Y., Jia J., Suo T., Chen Q., Liu Y.J., Tang J. PLoS ONE 6:E24367-E24367(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN AUTOPHAGY, SUBCELLULAR LOCATION, UBIQUITINATION BY RNF185.
+	Additional computationally mapped references.

Web resources

NIEHS-SNPs

Cross-references

Sequence databases
EMBL GenBank DDBJ	U15172 mRNA. Translation: AAC00020.1. AF083956 mRNA. Translation: AAC33124.1. AF083957 mRNA. Translation: AAC33125.1. AF083958 mRNA. Translation: AAC33126.1. AY216799 mRNA. Translation: AAO91805.1. AY246554 Genomic DNA. Translation: AAO61090.1. CH471062 Genomic DNA. Translation: EAW61405.1. CH471062 Genomic DNA. Translation: EAW61406.1. CH471062 Genomic DNA. Translation: EAW61408.1. CH471062 Genomic DNA. Translation: EAW61409.1. CH471062 Genomic DNA. Translation: EAW61410.1. CH471062 Genomic DNA. Translation: EAW61411.1. CH471062 Genomic DNA. Translation: EAW61412.1. CH471062 Genomic DNA. Translation: EAW61413.1. BC010959 mRNA. Translation: AAH10959.1.
IPI	IPI00030397. IPI00215701. IPI00215703. IPI00215704.
PIR	I38863.
RefSeq	NP_001196.2. NM_001205.2. NP_053581.2. NM_013978.2. NP_053582.2. NM_013979.2. NP_053583.2. NM_013980.2.
UniGene	Hs.145726.
3D structure databases
ProteinModelPortal	Q12981.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000231668.
PTM databases
PhosphoSite	Q12981.
Proteomic databases
PaxDb	Q12981.
PRIDE	Q12981.
Protocols and materials databases
DNASU	662.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000231668; ENSP00000231668; ENSG00000113734. ENST00000351486; ENSP00000239215; ENSG00000113734. ENST00000352523; ENSP00000239214; ENSG00000113734. ENST00000393770; ENSP00000377365; ENSG00000113734.
GeneID	662.
KEGG	hsa:662.
UCSC	uc003mci.4. human. uc003mcj.4. human. uc003mck.4. human. uc003mcl.4. human.
Organism-specific databases
CTD	662.
GeneCards	GC05P172504.
HGNC	HGNC:1082. BNIP1.
HPA	HPA008009.
MIM	603291. gene.
neXtProt	NX_Q12981.
PharmGKB	PA25392.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG73997.
HOVERGEN	HBG050708.
KO	K08497.
OMA	AMTGTIQ.
OrthoDB	EOG4BRWMR.
Gene expression databases
ArrayExpress	Q12981.
Bgee	Q12981.
CleanEx	HS_BNIP1.
Genevestigator	Q12981.
GermOnline	ENSG00000113734. Homo sapiens.
Family and domain databases
InterPro	IPR005606. Sec20. [Graphical view]
Pfam	PF03908. Sec20. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	BNIP1. human.
GenomeRNAi	662.
NextBio	2694.
SOURCE	Search...

Entry information

Entry name

SEC20_HUMAN

Accession

Primary (citable) accession number: Q12981
Secondary accession number(s): D3DQM3 , D3DQM4, D3DQM5, D3DQM6, O75622, O75623, O75624, Q6K044, Q96FG4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	April 18, 2006
Last modified:	April 3, 2013
This is version 109 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.