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Protein

Protein tyrosine phosphatase type IVA 2

Gene

PTP4A2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein tyrosine phosphatase which stimulates progression from G1 into S phase during mitosis. Promotes tumors. Inhibits geranylgeranyl transferase type II activity by blocking the association between RABGGTA and RABGGTB.1 Publication

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Enzyme regulationi

Inhibited by sodium orthovanadate and pentamidine.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei69Proton donorBy similarity1
Active sitei101Phosphocysteine intermediate1
Binding sitei107SubstrateBy similarity1

GO - Molecular functioni

  • prenylated protein tyrosine phosphatase activity Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Enzyme and pathway databases

BioCyciZFISH:G66-32373-MONOMER.
SIGNORiQ12974.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein tyrosine phosphatase type IVA 2 (EC:3.1.3.48)
Alternative name(s):
HU-PP-1
OV-1
PTP(CAAXII)
Protein-tyrosine phosphatase 4a2
Protein-tyrosine phosphatase of regenerating liver 2
Short name:
PRL-2
Gene namesi
Name:PTP4A2
Synonyms:PRL2, PTPCAAX2
ORF Names:BM-008
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:9635. PTP4A2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • early endosome Source: UniProtKB-SubCell
  • extracellular exosome Source: UniProtKB
  • nucleus Source: GO_Central
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi164 – 167Missing : Locates in the nucleus and cytosol. No interaction with RABGGTB. 1 Publication4
Mutagenesisi165C → S: No effect on interaction with RABGGTB. 1 Publication1

Organism-specific databases

DisGeNETi8073.
OpenTargetsiENSG00000184007.
PharmGKBiPA33978.

Chemistry databases

ChEMBLiCHEMBL1075105.

Polymorphism and mutation databases

BioMutaiPTP4A2.
DMDMi68566159.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000947851 – 164Protein tyrosine phosphatase type IVA 2Add BLAST164
PropeptideiPRO_0000396731165 – 167Removed in mature formCurated3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi46 ↔ 101By similarity
Modified residuei164Cysteine methyl esterCurated1
Lipidationi164S-farnesyl cysteine2 Publications1

Post-translational modificationi

Farnesylated. Farnesylation is required for membrane targeting and for interaction with RABGGTB. Unfarnesylated forms are redirected to the nucleus and cytosol.

Keywords - PTMi

Disulfide bond, Lipoprotein, Methylation, Prenylation

Proteomic databases

EPDiQ12974.
MaxQBiQ12974.
PaxDbiQ12974.
PeptideAtlasiQ12974.
PRIDEiQ12974.

PTM databases

DEPODiQ12974.
iPTMnetiQ12974.
PhosphoSitePlusiQ12974.

Expressioni

Tissue specificityi

Ubiquitously expressed, with highest levels in skeletal muscle, heart and thymus. Overexpressed in prostate tumor tissue.3 Publications

Gene expression databases

BgeeiENSG00000184007.
CleanExiHS_PTP4A2.
ExpressionAtlasiQ12974. baseline and differential.
GenevisibleiQ12974. HS.

Organism-specific databases

HPAiCAB011204.
HPA003281.

Interactioni

Subunit structurei

In contrast to PTP4A1 and PTP4A3, does not interact with tubulin. Interacts with RABGGTB.1 Publication

Protein-protein interaction databases

BioGridi113747. 20 interactors.
IntActiQ12974. 3 interactors.
MINTiMINT-2806089.
STRINGi9606.ENSP00000344909.

Chemistry databases

BindingDBiQ12974.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5K22X-ray3.00A1-163[»]
5K23X-ray2.96A1-167[»]
5K25X-ray3.05A1-167[»]
ProteinModelPortaliQ12974.
SMRiQ12974.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini79 – 145Tyrosine-protein phosphataseAdd BLAST67

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni102 – 107Phosphate bindingBy similarity6

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2836. Eukaryota.
ENOG4111I7J. LUCA.
GeneTreeiENSGT00390000009788.
HOGENOMiHOG000231265.
HOVERGENiHBG071295.
InParanoidiQ12974.
KOiK18041.
OMAiAPIEKEG.
OrthoDBiEOG091G0MYM.
PhylomeDBiQ12974.
TreeFamiTF313384.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR029021. Prot-tyrosine_phosphatase-like.
IPR000242. PTPase_domain.
IPR003595. Tyr_Pase_cat.
IPR000387. TYR_PHOSPHATASE_dom.
[Graphical view]
PfamiPF00102. Y_phosphatase. 1 hit.
[Graphical view]
SMARTiSM00404. PTPc_motif. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS50056. TYR_PHOSPHATASE_2. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q12974-1) [UniParc]FASTAAdd to basket
Also known as: Ptp-IV1a, Ptp-IV1b

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNRPAPVEIS YENMRFLITH NPTNATLNKF TEELKKYGVT TLVRVCDATY
60 70 80 90 100
DKAPVEKEGI HVLDWPFDDG APPPNQIVDD WLNLLKTKFR EEPGCCVAVH
110 120 130 140 150
CVAGLGRAPV LVALALIECG MKYEDAVQFI RQKRRGAFNS KQLLYLEKYR
160
PKMRLRFRDT NGHCCVQ
Length:167
Mass (Da):19,127
Last modified:November 1, 1996 - v1
Checksum:iE97B88BF87B87943
GO
Isoform 2 (identifier: Q12974-2) [UniParc]FASTAAdd to basket
Also known as: PTP4Ar

The sequence of this isoform differs from the canonical sequence as follows:
     64-82: DWPFDDGAPPPNQIVDDWL → KKKGSVQFQTAALFGEIPT
     83-167: Missing.

Show »
Length:82
Mass (Da):9,241
Checksum:i9B230FA7EA3F8A57
GO
Isoform 3 (identifier: Q12974-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     33-63: Missing.

Note: No experimental confirmation available.
Show »
Length:136
Mass (Da):15,669
Checksum:i906A2AF8CDF7C2C5
GO
Isoform 4 (identifier: Q12974-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     108-132: Missing.

Note: No experimental confirmation available. Gene prediction based on EST data.
Show »
Length:142
Mass (Da):16,367
Checksum:i777A719A36214008
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti13N → D in AAB39331 (PubMed:8661118).Curated1
Sequence conflicti53A → D in AAB59575 (PubMed:7490091).Curated1
Sequence conflicti102V → F in BAG59751 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04481333 – 63Missing in isoform 3. 1 PublicationAdd BLAST31
Alternative sequenceiVSP_01440464 – 82DWPFD…VDDWL → KKKGSVQFQTAALFGEIPT in isoform 2. CuratedAdd BLAST19
Alternative sequenceiVSP_01440583 – 167Missing in isoform 2. CuratedAdd BLAST85
Alternative sequenceiVSP_055056108 – 132Missing in isoform 4. CuratedAdd BLAST25

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14603 mRNA. Translation: AAA90979.1.
U48297 mRNA. Translation: AAB40598.1.
L48722 Genomic DNA. Translation: AAB42169.1.
L48723 Genomic DNA. Translation: AAB42170.1.
L48937 Genomic DNA. Translation: AAB39331.1.
AF208850 mRNA. Translation: AAF64264.1.
AK292703 mRNA. Translation: BAF85392.1.
AK297280 mRNA. Translation: BAG59751.1.
AL136115 Genomic DNA. Translation: CAI21726.1.
CH471059 Genomic DNA. Translation: EAX07578.1.
CH471059 Genomic DNA. Translation: EAX07579.1.
CH471059 Genomic DNA. Translation: EAX07582.1.
CH471059 Genomic DNA. Translation: EAX07583.1.
BC070182 mRNA. Translation: AAH70182.1.
L39000 mRNA. Translation: AAB59575.1.
CCDSiCCDS348.1. [Q12974-1]
CCDS53292.1. [Q12974-3]
CCDS59193.1. [Q12974-4]
PIRiI68523.
RefSeqiNP_001182029.1. NM_001195100.1. [Q12974-4]
NP_001182030.1. NM_001195101.1. [Q12974-3]
NP_536316.1. NM_080391.3. [Q12974-1]
XP_005271288.1. XM_005271231.3. [Q12974-4]
XP_005271289.1. XM_005271232.3. [Q12974-3]
XP_016857890.1. XM_017002401.1. [Q12974-3]
UniGeneiHs.470477.
Hs.713025.

Genome annotation databases

EnsembliENST00000457805; ENSP00000409260; ENSG00000184007. [Q12974-3]
ENST00000602683; ENSP00000473490; ENSG00000184007. [Q12974-4]
ENST00000602725; ENSP00000473259; ENSG00000184007. [Q12974-1]
GeneIDi8073.
KEGGihsa:8073.
UCSCiuc001btx.3. human. [Q12974-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14603 mRNA. Translation: AAA90979.1.
U48297 mRNA. Translation: AAB40598.1.
L48722 Genomic DNA. Translation: AAB42169.1.
L48723 Genomic DNA. Translation: AAB42170.1.
L48937 Genomic DNA. Translation: AAB39331.1.
AF208850 mRNA. Translation: AAF64264.1.
AK292703 mRNA. Translation: BAF85392.1.
AK297280 mRNA. Translation: BAG59751.1.
AL136115 Genomic DNA. Translation: CAI21726.1.
CH471059 Genomic DNA. Translation: EAX07578.1.
CH471059 Genomic DNA. Translation: EAX07579.1.
CH471059 Genomic DNA. Translation: EAX07582.1.
CH471059 Genomic DNA. Translation: EAX07583.1.
BC070182 mRNA. Translation: AAH70182.1.
L39000 mRNA. Translation: AAB59575.1.
CCDSiCCDS348.1. [Q12974-1]
CCDS53292.1. [Q12974-3]
CCDS59193.1. [Q12974-4]
PIRiI68523.
RefSeqiNP_001182029.1. NM_001195100.1. [Q12974-4]
NP_001182030.1. NM_001195101.1. [Q12974-3]
NP_536316.1. NM_080391.3. [Q12974-1]
XP_005271288.1. XM_005271231.3. [Q12974-4]
XP_005271289.1. XM_005271232.3. [Q12974-3]
XP_016857890.1. XM_017002401.1. [Q12974-3]
UniGeneiHs.470477.
Hs.713025.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5K22X-ray3.00A1-163[»]
5K23X-ray2.96A1-167[»]
5K25X-ray3.05A1-167[»]
ProteinModelPortaliQ12974.
SMRiQ12974.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113747. 20 interactors.
IntActiQ12974. 3 interactors.
MINTiMINT-2806089.
STRINGi9606.ENSP00000344909.

Chemistry databases

BindingDBiQ12974.
ChEMBLiCHEMBL1075105.

PTM databases

DEPODiQ12974.
iPTMnetiQ12974.
PhosphoSitePlusiQ12974.

Polymorphism and mutation databases

BioMutaiPTP4A2.
DMDMi68566159.

Proteomic databases

EPDiQ12974.
MaxQBiQ12974.
PaxDbiQ12974.
PeptideAtlasiQ12974.
PRIDEiQ12974.

Protocols and materials databases

DNASUi8073.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000457805; ENSP00000409260; ENSG00000184007. [Q12974-3]
ENST00000602683; ENSP00000473490; ENSG00000184007. [Q12974-4]
ENST00000602725; ENSP00000473259; ENSG00000184007. [Q12974-1]
GeneIDi8073.
KEGGihsa:8073.
UCSCiuc001btx.3. human. [Q12974-1]

Organism-specific databases

CTDi8073.
DisGeNETi8073.
GeneCardsiPTP4A2.
HGNCiHGNC:9635. PTP4A2.
HPAiCAB011204.
HPA003281.
MIMi601584. gene.
neXtProtiNX_Q12974.
OpenTargetsiENSG00000184007.
PharmGKBiPA33978.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2836. Eukaryota.
ENOG4111I7J. LUCA.
GeneTreeiENSGT00390000009788.
HOGENOMiHOG000231265.
HOVERGENiHBG071295.
InParanoidiQ12974.
KOiK18041.
OMAiAPIEKEG.
OrthoDBiEOG091G0MYM.
PhylomeDBiQ12974.
TreeFamiTF313384.

Enzyme and pathway databases

BioCyciZFISH:G66-32373-MONOMER.
SIGNORiQ12974.

Miscellaneous databases

GeneWikiiPTP4A2.
GenomeRNAii8073.
PROiQ12974.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000184007.
CleanExiHS_PTP4A2.
ExpressionAtlasiQ12974. baseline and differential.
GenevisibleiQ12974. HS.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR029021. Prot-tyrosine_phosphatase-like.
IPR000242. PTPase_domain.
IPR003595. Tyr_Pase_cat.
IPR000387. TYR_PHOSPHATASE_dom.
[Graphical view]
PfamiPF00102. Y_phosphatase. 1 hit.
[Graphical view]
SMARTiSM00404. PTPc_motif. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS50056. TYR_PHOSPHATASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTP4A2_HUMAN
AccessioniPrimary (citable) accession number: Q12974
Secondary accession number(s): A8K9I8
, B4DM39, D3DPP0, E9PGJ6, O00649, Q15197, Q15259, Q15260, Q15261, R4GN50
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 156 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

A processed pseudogene with 96% sequence identity was found in the BRCA1 (113705) region of 17q21.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.