Q12968 (NFAC3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nuclear factor of activated T-cells, cytoplasmic 3
Short name=NF-ATc3
Short name=NFATc3

Alternative name(s):
NFATx
T-cell transcription factor NFAT4
Short name=NF-AT4

Gene names

Name:	NFATC3
Synonyms:	NFAT4

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1075 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a regulator of transcriptional activation. Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2. Ref.9
Subunit structure	Member of the multicomponent NFATC transcription complex that consists of at least two components, a pre-existing cytoplasmic component NFATC2 and an inducible nuclear component NFATC1. Other members such as NFATC4, NFATC3 or members of the activating protein-1 family, MAF, GATA4 and Cbp/p300 can also bind the complex. NFATC proteins bind to DNA as monomers.
Subcellular location	Cytoplasm. Nucleus. Note: Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription. Ref.9
Tissue specificity	Isoform 1 is predominantly expressed in thymus and is also found in peripheral blood leukocytes and kidney. Isoform 2 is predominantly expressed in skeletal muscle and is also found in thymus, kidney, testis, spleen, prostate, ovary, small intestine, heart, placenta and pancreas. Isoform 3 is expressed in thymus and kidney. Isoform 4 is expressed in thymus and skeletal muscle.
Domain	Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.
Post-translational modification	Phosphorylated by NFATC-kinase; dephosphorylated by calcineurin By similarity.
Sequence similarities	Contains 1 RHD (Rel-like) domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat
Ligand	DNA-binding
Molecular function	Activator
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cellular respiration Inferred from electronic annotation. Source: Compara cellular response to lithium ion Inferred from electronic annotation. Source: Compara heart development Inferred from electronic annotation. Source: Compara inflammatory response Traceable author statement Ref.2. Source: ProtInc muscle cell development Inferred from electronic annotation. Source: Compara patterning of blood vessels Inferred from electronic annotation. Source: Compara positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay Ref.9. Source: UniProtKB smooth muscle cell differentiation Inferred from electronic annotation. Source: Compara transcription from RNA polymerase II promoter Traceable author statement Ref.2. Source: ProtInc
Cellular_component	cytosol Traceable author statement. Source: Reactome nucleolus Inferred from direct assay. Source: HPA plasma membrane Inferred from direct assay. Source: HPA
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW chromatin binding Inferred from electronic annotation. Source: Compara sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q12968-1)

Also known as: X1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q12968-2)

Also known as: X2; C;

The sequence of this isoform differs from the canonical sequence as follows:
     1036-1075: VNEIIGRDMSQISVSQGAGVSRQAPLPSPESLDLGRSDGL → DQFISDLEHQPSGSAEKWPNHSVLSCPAPFWRI

Isoform 3 (identifier: Q12968-3)

Also known as: X3;

The sequence of this isoform differs from the canonical sequence as follows:
     1036-1075: VNEIIGRDMSQISVSQGAGVSRQAPLPSPESLDLGRSDGL → DLFTSNNFDLLQLRPTFWPVPAGRYLRNLE

Isoform 4 (identifier: Q12968-4)

Also known as: X4;

The sequence of this isoform differs from the canonical sequence as follows:
     1036-1075: VNEIIGRDMSQISVSQGAGVSRQAPLPSPESLDLGRSDGL → GKFISDMFLK

Isoform 5 (identifier: Q12968-5)

Also known as: A;

The sequence of this isoform differs from the canonical sequence as follows:
     700-1075: VLMKQEHREE...SLDLGRSDGL → GTRSHDGLL

Isoform 6 (identifier: Q12968-6)

Also known as: B;

The sequence of this isoform differs from the canonical sequence as follows:
     717-1075: SLPVPHPAQT...SLDLGRSDGL → TLPQTSRQTLLGSQPPSASPPTV

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.6

Chain

2 – 1075

1074

Nuclear factor of activated T-cells, cytoplasmic 3

PRO_0000205180

Regions

Repeat

207 – 223

17

1

Repeat

236 – 252

17

2

Repeat

292 – 308

17

3

Domain

415 – 596

182

RHD

DNA binding

444 – 451

8

Region

109 – 114

6

Calcineurin-binding

Region

207 – 308

102

3 X SP repeats

Motif

273 – 275

3

Nuclear localization signal

Motif

686 – 688

3

Nuclear localization signal

Motif

1032 – 1041

10

Nuclear export signal

Compositional bias

24 – 29

6

Poly-Pro

Amino acid modifications

Modified residue

2

1

N-acetylthreonine Ref.6

Natural variations

Alternative sequence

700 – 1075

376

VLMKQ…RSDGL → GTRSHDGLL in isoform 5.

VSP_005598

Alternative sequence

717 – 1075

359

SLPVP…RSDGL → TLPQTSRQTLLGSQPPSASP PTV in isoform 6.

VSP_005599

Alternative sequence

1036 – 1075

40

VNEII…RSDGL → DQFISDLEHQPSGSAEKWPN HSVLSCPAPFWRI in isoform 2.

VSP_005600

Alternative sequence

1036 – 1075

40

VNEII…RSDGL → DLFTSNNFDLLQLRPTFWPV PAGRYLRNLE in isoform 3.

VSP_005601

Alternative sequence

1036 – 1075

40

VNEII…RSDGL → GKFISDMFLK in isoform 4.

VSP_005602

Natural variant

75

1

S → L.
Corresponds to variant rs2230092 [ dbSNP | Ensembl ].

VAR_051784

Natural variant

94

1

E → A.
Corresponds to variant rs3743736 [ dbSNP | Ensembl ].

VAR_051785

Natural variant

100

1

L → S.
Corresponds to variant rs2230093 [ dbSNP | Ensembl ].

VAR_051786

Natural variant

136

1

P → L.
Corresponds to variant rs2230094 [ dbSNP | Ensembl ].

VAR_051787

Natural variant

382

1

P → S.
Corresponds to variant rs2230095 [ dbSNP | Ensembl ].

VAR_051788

Experimental info

Sequence conflict

702

1

M → L in AAB46595. Ref.3

Sequence conflict

702

1

M → L in AAB46596. Ref.3

Sequence conflict

702

1

M → L in AAB46597. Ref.3

Sequence conflict

831

1

L → W in AAB46595. Ref.3

Sequence conflict

831

1

L → W in AAB46596. Ref.3

Sequence conflict

831

1

L → W in AAB46597. Ref.3

Sequence conflict

899

1

A → G in AAB46595. Ref.3

Sequence conflict

899

1

A → G in AAB46596. Ref.3

Sequence conflict

899

1

A → G in AAB46597. Ref.3

Sequence conflict

923

1

A → V in AAB46595. Ref.3

Sequence conflict

923

1

A → V in AAB46596. Ref.3

Sequence conflict

923

1

A → V in AAB46597. Ref.3

Sequence conflict

935

1

A → G in AAB46595. Ref.3

Sequence conflict

935

1

A → G in AAB46596. Ref.3

Sequence conflict

935

1

A → G in AAB46597. Ref.3

Sequence conflict

947

1

L → F in AAB46595. Ref.3

Sequence conflict

947

1

L → F in AAB46596. Ref.3

Sequence conflict

947

1

L → F in AAB46597. Ref.3

Sequence conflict

960

1

A → G in AAB46595. Ref.3

Sequence conflict

960

1

A → G in AAB46596. Ref.3

Sequence conflict

960

1

A → G in AAB46597. Ref.3

Secondary structure

1

.

.

.

1075

Helix Strand Turn

Details...

Helix

146 – 148

3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (X1) [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 0B25D61A2B56D898	FASTA	1,075	115,594
	10 20 30 40 50 60 MTTANCGAHD ELDFKLVFGE DGAPAPPPPG SRPADLEPDD CASIYIFNVD PPPSTLTTPL 70 80 90 100 110 120 CLPHHGLPSH SSVLSPSFQL QSHKNYEGTC EIPESKYSPL GGPKPFECPS IQITSISPNC 130 140 150 160 170 180 HQELDAHEDD LQINDPEREF LERPSRDHLY LPLEPSYRES SLSPSPASSI SSRSWFSDAS 190 200 210 220 230 240 SCESLSHIYD DVDSELNEAA ARFTLGSPLT SPGGSPGGCP GEETWHQQYG LGHSLSPRQS 250 260 270 280 290 300 PCHSPRSSVT DENWLSPRPA SGPSSRPTSP CGKRRHSSAE VCYAGSLSPH HSPVPSPGHS 310 320 330 340 350 360 PRGSVTEDTW LNASVHGGSG LGPAVFPFQY CVETDIPLKT RKTSEDQAAI LPGKLELCSD 370 380 390 400 410 420 DQGSLSPARE TSIDDGLGSQ YPLKKDSCGD QFLSVPSPFT WSKPKPGHTP IFRTSSLPPL 430 440 450 460 470 480 DWPLPAHFGQ CELKIEVQPK THHRAHYETE GSRGAVKAST GGHPVVKLLG YNEKPINLQM 490 500 510 520 530 540 FIGTADDRYL RPHAFYQVHR ITGKTVATAS QEIIIASTKV LEIPLLPENN MSASIDCAGI 550 560 570 580 590 600 LKLRNSDIEL RKGETDIGRK NTRVRLVFRV HIPQPSGKVL SLQIASIPVE CSQRSAQELP 610 620 630 640 650 660 HIEKYSINSC SVNGGHEMVV TGSNFLPESK IIFLEKGQDG RPQWEVEGKI IREKCQGAHI 670 680 690 700 710 720 VLEVPPYHNP AVTAAVQVHF YLCNGKRKKS QSQRFTYTPV LMKQEHREEI DLSSVPSLPV 730 740 750 760 770 780 PHPAQTQRPS SDSGCSHDSV LSGQRSLICS IPQTYASMVT SSHLPQLQCR DESVSKEQHM 790 800 810 820 830 840 IPSPIVHQPF QVTPTPPVGS SYQPMQTNVV YNGPTCLPIN AASSQEFDSV LFQQDATLSG 850 860 870 880 890 900 LVNLGCQPLS SIPFHSSNSG STGHLLAHTP HSVHTLPHLQ SMGYHCSNTG QRSLSSPVAD 910 920 930 940 950 960 QITGQPSSQL QPITYGPSHS GSATTASPAA SHPLASSPLS GPPSPQLQPM PYQSPSSGTA 970 980 990 1000 1010 1020 SSPSPATRMH SGQHSTQAQS TGQGGLSAPS SLICHSLCDP ASFPPDGATV SIKPEPEDRE 1030 1040 1050 1060 1070 PNFATIGLQD ITLDDVNEII GRDMSQISVS QGAGVSRQAP LPSPESLDLG RSDGL
	Isoform 2 (X2) (C) [UniParc]. Checksum: CD91817D06AD5D82	FASTA	1,068	115,237
	10 20 30 40 50 60 MTTANCGAHD ELDFKLVFGE DGAPAPPPPG SRPADLEPDD CASIYIFNVD PPPSTLTTPL 70 80 90 100 110 120 CLPHHGLPSH SSVLSPSFQL QSHKNYEGTC EIPESKYSPL GGPKPFECPS IQITSISPNC 130 140 150 160 170 180 HQELDAHEDD LQINDPEREF LERPSRDHLY LPLEPSYRES SLSPSPASSI SSRSWFSDAS 190 200 210 220 230 240 SCESLSHIYD DVDSELNEAA ARFTLGSPLT SPGGSPGGCP GEETWHQQYG LGHSLSPRQS 250 260 270 280 290 300 PCHSPRSSVT DENWLSPRPA SGPSSRPTSP CGKRRHSSAE VCYAGSLSPH HSPVPSPGHS 310 320 330 340 350 360 PRGSVTEDTW LNASVHGGSG LGPAVFPFQY CVETDIPLKT RKTSEDQAAI LPGKLELCSD 370 380 390 400 410 420 DQGSLSPARE TSIDDGLGSQ YPLKKDSCGD QFLSVPSPFT WSKPKPGHTP IFRTSSLPPL 430 440 450 460 470 480 DWPLPAHFGQ CELKIEVQPK THHRAHYETE GSRGAVKAST GGHPVVKLLG YNEKPINLQM 490 500 510 520 530 540 FIGTADDRYL RPHAFYQVHR ITGKTVATAS QEIIIASTKV LEIPLLPENN MSASIDCAGI 550 560 570 580 590 600 LKLRNSDIEL RKGETDIGRK NTRVRLVFRV HIPQPSGKVL SLQIASIPVE CSQRSAQELP 610 620 630 640 650 660 HIEKYSINSC SVNGGHEMVV TGSNFLPESK IIFLEKGQDG RPQWEVEGKI IREKCQGAHI 670 680 690 700 710 720 VLEVPPYHNP AVTAAVQVHF YLCNGKRKKS QSQRFTYTPV LMKQEHREEI DLSSVPSLPV 730 740 750 760 770 780 PHPAQTQRPS SDSGCSHDSV LSGQRSLICS IPQTYASMVT SSHLPQLQCR DESVSKEQHM 790 800 810 820 830 840 IPSPIVHQPF QVTPTPPVGS SYQPMQTNVV YNGPTCLPIN AASSQEFDSV LFQQDATLSG 850 860 870 880 890 900 LVNLGCQPLS SIPFHSSNSG STGHLLAHTP HSVHTLPHLQ SMGYHCSNTG QRSLSSPVAD 910 920 930 940 950 960 QITGQPSSQL QPITYGPSHS GSATTASPAA SHPLASSPLS GPPSPQLQPM PYQSPSSGTA 970 980 990 1000 1010 1020 SSPSPATRMH SGQHSTQAQS TGQGGLSAPS SLICHSLCDP ASFPPDGATV SIKPEPEDRE 1030 1040 1050 1060 PNFATIGLQD ITLDDDQFIS DLEHQPSGSA EKWPNHSVLS CPAPFWRI
	Isoform 3 (X3) [UniParc]. Checksum: B827F1D5CE01D033	FASTA	1,065	115,050
	10 20 30 40 50 60 MTTANCGAHD ELDFKLVFGE DGAPAPPPPG SRPADLEPDD CASIYIFNVD PPPSTLTTPL 70 80 90 100 110 120 CLPHHGLPSH SSVLSPSFQL QSHKNYEGTC EIPESKYSPL GGPKPFECPS IQITSISPNC 130 140 150 160 170 180 HQELDAHEDD LQINDPEREF LERPSRDHLY LPLEPSYRES SLSPSPASSI SSRSWFSDAS 190 200 210 220 230 240 SCESLSHIYD DVDSELNEAA ARFTLGSPLT SPGGSPGGCP GEETWHQQYG LGHSLSPRQS 250 260 270 280 290 300 PCHSPRSSVT DENWLSPRPA SGPSSRPTSP CGKRRHSSAE VCYAGSLSPH HSPVPSPGHS 310 320 330 340 350 360 PRGSVTEDTW LNASVHGGSG LGPAVFPFQY CVETDIPLKT RKTSEDQAAI LPGKLELCSD 370 380 390 400 410 420 DQGSLSPARE TSIDDGLGSQ YPLKKDSCGD QFLSVPSPFT WSKPKPGHTP IFRTSSLPPL 430 440 450 460 470 480 DWPLPAHFGQ CELKIEVQPK THHRAHYETE GSRGAVKAST GGHPVVKLLG YNEKPINLQM 490 500 510 520 530 540 FIGTADDRYL RPHAFYQVHR ITGKTVATAS QEIIIASTKV LEIPLLPENN MSASIDCAGI 550 560 570 580 590 600 LKLRNSDIEL RKGETDIGRK NTRVRLVFRV HIPQPSGKVL SLQIASIPVE CSQRSAQELP 610 620 630 640 650 660 HIEKYSINSC SVNGGHEMVV TGSNFLPESK IIFLEKGQDG RPQWEVEGKI IREKCQGAHI 670 680 690 700 710 720 VLEVPPYHNP AVTAAVQVHF YLCNGKRKKS QSQRFTYTPV LMKQEHREEI DLSSVPSLPV 730 740 750 760 770 780 PHPAQTQRPS SDSGCSHDSV LSGQRSLICS IPQTYASMVT SSHLPQLQCR DESVSKEQHM 790 800 810 820 830 840 IPSPIVHQPF QVTPTPPVGS SYQPMQTNVV YNGPTCLPIN AASSQEFDSV LFQQDATLSG 850 860 870 880 890 900 LVNLGCQPLS SIPFHSSNSG STGHLLAHTP HSVHTLPHLQ SMGYHCSNTG QRSLSSPVAD 910 920 930 940 950 960 QITGQPSSQL QPITYGPSHS GSATTASPAA SHPLASSPLS GPPSPQLQPM PYQSPSSGTA 970 980 990 1000 1010 1020 SSPSPATRMH SGQHSTQAQS TGQGGLSAPS SLICHSLCDP ASFPPDGATV SIKPEPEDRE 1030 1040 1050 1060 PNFATIGLQD ITLDDDLFTS NNFDLLQLRP TFWPVPAGRY LRNLE
	Isoform 4 (X4) [UniParc]. Checksum: 44CE97FCF4A4EEAD	FASTA	1,045	112,642
	10 20 30 40 50 60 MTTANCGAHD ELDFKLVFGE DGAPAPPPPG SRPADLEPDD CASIYIFNVD PPPSTLTTPL 70 80 90 100 110 120 CLPHHGLPSH SSVLSPSFQL QSHKNYEGTC EIPESKYSPL GGPKPFECPS IQITSISPNC 130 140 150 160 170 180 HQELDAHEDD LQINDPEREF LERPSRDHLY LPLEPSYRES SLSPSPASSI SSRSWFSDAS 190 200 210 220 230 240 SCESLSHIYD DVDSELNEAA ARFTLGSPLT SPGGSPGGCP GEETWHQQYG LGHSLSPRQS 250 260 270 280 290 300 PCHSPRSSVT DENWLSPRPA SGPSSRPTSP CGKRRHSSAE VCYAGSLSPH HSPVPSPGHS 310 320 330 340 350 360 PRGSVTEDTW LNASVHGGSG LGPAVFPFQY CVETDIPLKT RKTSEDQAAI LPGKLELCSD 370 380 390 400 410 420 DQGSLSPARE TSIDDGLGSQ YPLKKDSCGD QFLSVPSPFT WSKPKPGHTP IFRTSSLPPL 430 440 450 460 470 480 DWPLPAHFGQ CELKIEVQPK THHRAHYETE GSRGAVKAST GGHPVVKLLG YNEKPINLQM 490 500 510 520 530 540 FIGTADDRYL RPHAFYQVHR ITGKTVATAS QEIIIASTKV LEIPLLPENN MSASIDCAGI 550 560 570 580 590 600 LKLRNSDIEL RKGETDIGRK NTRVRLVFRV HIPQPSGKVL SLQIASIPVE CSQRSAQELP 610 620 630 640 650 660 HIEKYSINSC SVNGGHEMVV TGSNFLPESK IIFLEKGQDG RPQWEVEGKI IREKCQGAHI 670 680 690 700 710 720 VLEVPPYHNP AVTAAVQVHF YLCNGKRKKS QSQRFTYTPV LMKQEHREEI DLSSVPSLPV 730 740 750 760 770 780 PHPAQTQRPS SDSGCSHDSV LSGQRSLICS IPQTYASMVT SSHLPQLQCR DESVSKEQHM 790 800 810 820 830 840 IPSPIVHQPF QVTPTPPVGS SYQPMQTNVV YNGPTCLPIN AASSQEFDSV LFQQDATLSG 850 860 870 880 890 900 LVNLGCQPLS SIPFHSSNSG STGHLLAHTP HSVHTLPHLQ SMGYHCSNTG QRSLSSPVAD 910 920 930 940 950 960 QITGQPSSQL QPITYGPSHS GSATTASPAA SHPLASSPLS GPPSPQLQPM PYQSPSSGTA 970 980 990 1000 1010 1020 SSPSPATRMH SGQHSTQAQS TGQGGLSAPS SLICHSLCDP ASFPPDGATV SIKPEPEDRE 1030 1040 PNFATIGLQD ITLDDGKFIS DMFLK
	Isoform 5 (A) [UniParc]. Checksum: D96C59AEC592D0E0	FASTA	708	77,181
	10 20 30 40 50 60 MTTANCGAHD ELDFKLVFGE DGAPAPPPPG SRPADLEPDD CASIYIFNVD PPPSTLTTPL 70 80 90 100 110 120 CLPHHGLPSH SSVLSPSFQL QSHKNYEGTC EIPESKYSPL GGPKPFECPS IQITSISPNC 130 140 150 160 170 180 HQELDAHEDD LQINDPEREF LERPSRDHLY LPLEPSYRES SLSPSPASSI SSRSWFSDAS 190 200 210 220 230 240 SCESLSHIYD DVDSELNEAA ARFTLGSPLT SPGGSPGGCP GEETWHQQYG LGHSLSPRQS 250 260 270 280 290 300 PCHSPRSSVT DENWLSPRPA SGPSSRPTSP CGKRRHSSAE VCYAGSLSPH HSPVPSPGHS 310 320 330 340 350 360 PRGSVTEDTW LNASVHGGSG LGPAVFPFQY CVETDIPLKT RKTSEDQAAI LPGKLELCSD 370 380 390 400 410 420 DQGSLSPARE TSIDDGLGSQ YPLKKDSCGD QFLSVPSPFT WSKPKPGHTP IFRTSSLPPL 430 440 450 460 470 480 DWPLPAHFGQ CELKIEVQPK THHRAHYETE GSRGAVKAST GGHPVVKLLG YNEKPINLQM 490 500 510 520 530 540 FIGTADDRYL RPHAFYQVHR ITGKTVATAS QEIIIASTKV LEIPLLPENN MSASIDCAGI 550 560 570 580 590 600 LKLRNSDIEL RKGETDIGRK NTRVRLVFRV HIPQPSGKVL SLQIASIPVE CSQRSAQELP 610 620 630 640 650 660 HIEKYSINSC SVNGGHEMVV TGSNFLPESK IIFLEKGQDG RPQWEVEGKI IREKCQGAHI 670 680 690 700 VLEVPPYHNP AVTAAVQVHF YLCNGKRKKS QSQRFTYTPG TRSHDGLL
	Isoform 6 (B) [UniParc]. Checksum: 8AB8BAFA1A319E50	FASTA	739	80,582
	10 20 30 40 50 60 MTTANCGAHD ELDFKLVFGE DGAPAPPPPG SRPADLEPDD CASIYIFNVD PPPSTLTTPL 70 80 90 100 110 120 CLPHHGLPSH SSVLSPSFQL QSHKNYEGTC EIPESKYSPL GGPKPFECPS IQITSISPNC 130 140 150 160 170 180 HQELDAHEDD LQINDPEREF LERPSRDHLY LPLEPSYRES SLSPSPASSI SSRSWFSDAS 190 200 210 220 230 240 SCESLSHIYD DVDSELNEAA ARFTLGSPLT SPGGSPGGCP GEETWHQQYG LGHSLSPRQS 250 260 270 280 290 300 PCHSPRSSVT DENWLSPRPA SGPSSRPTSP CGKRRHSSAE VCYAGSLSPH HSPVPSPGHS 310 320 330 340 350 360 PRGSVTEDTW LNASVHGGSG LGPAVFPFQY CVETDIPLKT RKTSEDQAAI LPGKLELCSD 370 380 390 400 410 420 DQGSLSPARE TSIDDGLGSQ YPLKKDSCGD QFLSVPSPFT WSKPKPGHTP IFRTSSLPPL 430 440 450 460 470 480 DWPLPAHFGQ CELKIEVQPK THHRAHYETE GSRGAVKAST GGHPVVKLLG YNEKPINLQM 490 500 510 520 530 540 FIGTADDRYL RPHAFYQVHR ITGKTVATAS QEIIIASTKV LEIPLLPENN MSASIDCAGI 550 560 570 580 590 600 LKLRNSDIEL RKGETDIGRK NTRVRLVFRV HIPQPSGKVL SLQIASIPVE CSQRSAQELP 610 620 630 640 650 660 HIEKYSINSC SVNGGHEMVV TGSNFLPESK IIFLEKGQDG RPQWEVEGKI IREKCQGAHI 670 680 690 700 710 720 VLEVPPYHNP AVTAAVQVHF YLCNGKRKKS QSQRFTYTPV LMKQEHREEI DLSSVPTLPQ 730 TSRQTLLGSQ PPSASPPTV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of two new members of the NF-AT gene family and functional characterization of the NF-AT proteins." Hoey T., Sun Y.-L., Williamson K., Xu X. Immunity 2:461-472(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2; 5 AND 6). Tissue: Skeletal muscle and T-cell.
[2]	"NFATx, a novel member of the nuclear factor of activated T cells family that is expressed predominantly in the thymus." Masuda E.S., Naito Y., Tokumitsu H., Campbell D., Saito F., Hannum C., Arai K., Arai N. Mol. Cell. Biol. 15:2697-2706(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: T-cell.
[3]	"Carboxy-terminal 15 amino acids sequence of NFATx1 is possibly created by tissue-specific splicing and is essential for transactivation activity in T cells." Imamura R., Masuda E.S., Naito Y., Imai S., Fujino T., Takano T., Arai K., Arai N. J. Immunol. 161:3455-3463(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2; 3 AND 4). Tissue: Fibroblast and T-cell.
[4]	"Genome duplications and other features in 12 Mb of DNA sequence from human chromosome 16p and 16q." Loftus B.J., Kim U.-J., Sneddon V.P., Kalush F., Brandon R., Fuhrmann J., Mason T., Crosby M.L., Barnstead M., Cronin L., Mays A.D., Cao Y., Xu R.X., Kang H.-L., Mitchell S., Eichler E.E., Harris P.C., Venter J.C., Adams M.D. Genomics 60:295-308(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORM 4).
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Kidney.
[6]	Bienvenut W.V., Waridel P., Quadroni M. Submitted (MAR-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-15; 468-488; 505-519 AND 579-594, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT THR-2, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[7]	"Intramolecular masking of nuclear import signal on NF-AT4 by casein kinase I and MEKK1." Zhu J., Shibasaki F., Price R., Guillemot J.-C., Yano T., Doetsch V., Wagner G., Ferrara P., McKeon F. Cell 93:851-861(1998) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS.
[8]	"Generic signals and specific outcomes: signaling through Ca2+, calcineurin, and NF-AT." Crabtree G.R. Cell 96:611-614(1999) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
[9]	"CHP2 activates the calcineurin/nuclear factor of activated T cells signaling pathway and enhances the oncogenic potential of HEK293 cells." Li G.D., Zhang X., Li R., Wang Y.D., Wang Y.L., Han K.J., Qian X.P., Yang C.G., Liu P., Wei Q., Chen W.F., Zhang J., Zhang Y. J. Biol. Chem. 283:32660-32668(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L41067 mRNA. Translation: AAA79174.1.
U14510 mRNA. Translation: AAA86308.1.
U85428 mRNA. Translation: AAB46595.1.
U85429 mRNA. Translation: AAB46596.1.
U85430 mRNA. Translation: AAB46597.1.
AC130462 Genomic DNA. No translation available.
BC001050 mRNA. Translation: AAH01050.1.

IPI

IPI00005240.
IPI00030369.
IPI00215624.
IPI00215625.
IPI00215626.
IPI00215627.

PIR

A57377.

RefSeq

NP_004546.1. NM_004555.3.
NP_775186.1. NM_173163.2.
NP_775188.1. NM_173165.2.

UniGene

Hs.436585.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2XRW	X-ray	1.33	B	141-154	[»]
2XS0	X-ray	2.60	B	141-154	[»]

ProteinModelPortal

Q12968.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q12968. 1 interaction.

STRING

9606.ENSP00000300659.

PTM databases

PhosphoSite

Q12968.

Polymorphism databases

DMDM

9087155.

Proteomic databases

PaxDb

Q12968.

PRIDE

Q12968.

Protocols and materials databases

DNASU

4775.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000329524; ENSP00000331324; ENSG00000072736.
ENST00000346183; ENSP00000300659; ENSG00000072736.
ENST00000349223; ENSP00000264008; ENSG00000072736.

GeneID

4775.

KEGG

hsa:4775.

UCSC

uc002evn.2. human.
uc002evo.2. human.

Organism-specific databases

CTD

4775.

GeneCards

GC16P068119.

HGNC

HGNC:7777. NFATC3.

HPA

CAB004514.
HPA023844.

MIM

602698. gene.

neXtProt

NX_Q12968.

PharmGKB

PA247.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG70055.

HOGENOM

HOG000231780.

HOVERGEN

HBG069754.

InParanoid

Q12968.

KO

K04446.

OMA

ASSQEFD.

PhylomeDB

Q12968.

Enzyme and pathway databases

Pathway_Interaction_DB

nfat_tfpathway. Calcineurin-regulated NFAT-dependent transcription in lymphocytes.
tcrcalciumpathway. Calcium signaling in the CD4+ TCR pathway.
cd8tcrdownstreampathway. Downstream signaling in naive CD8+ T cells.
nfat_3pathway. Role of Calcineurin-dependent NFAT signaling in lymphocytes.

Reactome

REACT_118664. Calcineurin Dephosphorylates NFATC1/2/3.

Gene expression databases

ArrayExpress

Q12968.

Bgee

Q12968.

CleanEx

HS_NFATC3.

Genevestigator

Q12968.

GermOnline

ENSG00000072736. Homo sapiens.

Family and domain databases

Gene3D

2.60.40.10. 1 hit.
2.60.40.340. 1 hit.

InterPro

IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002909. IPT_TIG_rcpt.
IPR008366. NFAT.
IPR008967. p53-like_TF_DNA-bd.
IPR011539. RHD.
[Graphical view]

PANTHER

PTHR12533. PTHR12533. 1 hit.

Pfam

PF00554. RHD. 1 hit.
[Graphical view]

PRINTS

PR01789. NUCFACTORATC.

SMART

SM00429. IPT. 1 hit.
[Graphical view]

SUPFAM

SSF81296. Ig_E-set. 1 hit.
SSF49417. P53_like_DNA_bnd. 1 hit.

PROSITE

PS01204. REL_1. False negative.
PS50254. REL_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

NFATC3. human.

GenomeRNAi

4775.

NextBio

18410.

SOURCE

Search...

Entry information

Entry name

NFAC3_HUMAN

Accession

Primary (citable) accession number: Q12968
Secondary accession number(s): O75211 , Q14516, Q99840, Q99841, Q99842

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 134 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.