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Protein

Transcription initiation factor TFIID subunit 10

Gene

TAF10

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

TAFs are components of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TIIFD is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors.

GO - Molecular functioni

  • enzyme binding Source: UniProtKB
  • estrogen receptor binding Source: UniProtKB
  • RNA polymerase binding Source: UniProtKB
  • transcription coactivator activity Source: UniProtKB

GO - Biological processi

  • DNA-templated transcription, initiation Source: UniProtKB
  • histone deubiquitination Source: UniProtKB
  • histone H3 acetylation Source: UniProtKB
  • protein homooligomerization Source: UniProtKB
  • regulation of signal transduction by p53 class mediator Source: Reactome
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription elongation from RNA polymerase II promoter Source: Reactome
  • transcription from RNA polymerase II promoter Source: UniProtKB
  • transcription initiation from RNA polymerase II promoter Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-167161. HIV Transcription Initiation.
R-HSA-167162. RNA Polymerase II HIV Promoter Escape.
R-HSA-167172. Transcription of the HIV genome.
R-HSA-3214847. HATs acetylate histones.
R-HSA-674695. RNA Polymerase II Pre-transcription Events.
R-HSA-6804756. Regulation of TP53 Activity through Phosphorylation.
R-HSA-73776. RNA Polymerase II Promoter Escape.
R-HSA-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-HSA-75953. RNA Polymerase II Transcription Initiation.
R-HSA-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor TFIID subunit 10
Alternative name(s):
STAF28
Transcription initiation factor TFIID 30 kDa subunit
Short name:
TAF(II)30
Short name:
TAFII-30
Short name:
TAFII30
Gene namesi
Name:TAF10
Synonyms:TAF2A, TAF2H, TAFII30
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:11543. TAF10.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: HGNC
  • nucleoplasm Source: Reactome
  • nucleus Source: UniProtKB
  • PCAF complex Source: UniProtKB
  • perinuclear region of cytoplasm Source: HGNC
  • STAGA complex Source: UniProtKB
  • transcription factor TFIID complex Source: UniProtKB
  • transcription factor TFTC complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi189 – 1891K → Q: Abolishes methylation. 1 Publication

Organism-specific databases

PharmGKBiPA36318.

Polymorphism and mutation databases

BioMutaiTAF10.
DMDMi3024688.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 218217Transcription initiation factor TFIID subunit 10PRO_0000118897Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineCombined sources
Modified residuei44 – 441PhosphoserineCombined sources
Modified residuei48 – 481PhosphothreonineCombined sources
Modified residuei189 – 1891N6-methyllysine2 Publications

Post-translational modificationi

Monomethylated at Lys-189 by SETD7, leading to increase its affinity for RNA polymerase II.

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiQ12962.
MaxQBiQ12962.
PaxDbiQ12962.
PeptideAtlasiQ12962.
PRIDEiQ12962.

PTM databases

iPTMnetiQ12962.
PhosphoSiteiQ12962.

Expressioni

Gene expression databases

BgeeiENSG00000166337.
CleanExiHS_TAF10.
ExpressionAtlasiQ12962. baseline and differential.
GenevisibleiQ12962. HS.

Organism-specific databases

HPAiCAB022405.
HPA004148.

Interactioni

Subunit structurei

TFIID and PCAF are composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). TBP is not part of TFTC. Component of the PCAF complex, at least composed of TADA2L/ADA2, TADA3L/ADA3, SUPT3H, TAF5L TAF6L, TAF9, TAF10, TAF12 and TRRAP. Component of the TFTC-HAT complex, at least composed of TAF5L, TAF6L, TADA3L, SUPT3H, TAF2, TAF4, TAF5, GCN5L2/GCN5, TAF10 and TRRAP. Component of the STAGA transcription coactivator-HAT complex, at least composed of SUPT3H, GCN5L2, TAF5L, TAF6L, SUPT7L, TADA3L, TAD1L, TAF10, TAF12, TRRAP and TAF9. The STAGA core complex is associated with a subcomplex required for histone deubiquitination composed of ATXN7L3, ENY2 and USP22. Interacts with TAF3.7 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ATXN7O152656EBI-708376,EBI-708350
SETD7Q8WTS62EBI-708376,EBI-1268586

GO - Molecular functioni

  • enzyme binding Source: UniProtKB
  • estrogen receptor binding Source: UniProtKB
  • RNA polymerase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi112744. 71 interactions.
DIPiDIP-297N.
IntActiQ12962. 17 interactions.
MINTiMINT-1425043.
STRINGi9606.ENSP00000299424.

Structurei

Secondary structure

1
218
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi115 – 1217Combined sources
Helixi122 – 1243Combined sources
Helixi132 – 14110Combined sources
Helixi149 – 17426Combined sources
Helixi197 – 2004Combined sources
Helixi201 – 2077Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2F69X-ray1.30B186-195[»]
3M53X-ray1.85B186-195[»]
3M54X-ray1.60B186-195[»]
3M55X-ray1.55B186-195[»]
3M56X-ray1.65B186-195[»]
3M57X-ray1.70B186-195[»]
3M58X-ray1.40B186-195[»]
3M59X-ray1.70B186-195[»]
3M5AX-ray1.75B186-195[»]
4J7FX-ray1.59B186-195[»]
4J7IX-ray2.56B186-195[»]
4J83X-ray1.70B186-195[»]
4J8OX-ray1.63B186-195[»]
4WV4X-ray1.91A112-212[»]
5EG2X-ray1.55B186-195[»]
ProteinModelPortaliQ12962.
SMRiQ12962. Positions 113-212.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi187 – 1893[KR]-[STA]-K motif

Domaini

The [KR]-[STA]-K motif is specifically recognized by the SETD7 methyltransferase.

Sequence similaritiesi

Belongs to the TAF10 family.Curated

Phylogenomic databases

eggNOGiKOG3423. Eukaryota.
COG5162. LUCA.
GeneTreeiENSGT00390000009368.
HOGENOMiHOG000285966.
HOVERGENiHBG079227.
InParanoidiQ12962.
KOiK03134.
OMAiDNTIGNP.
OrthoDBiEOG091G0UWA.
PhylomeDBiQ12962.
TreeFamiTF313156.

Family and domain databases

CDDicd07982. TAF10. 1 hit.
InterProiIPR003923. TFIID_30kDa.
[Graphical view]
PANTHERiPTHR21242. PTHR21242. 1 hit.
PfamiPF03540. TFIID_30kDa. 1 hit.
[Graphical view]
PIRSFiPIRSF017246. TFIID_TAF10. 1 hit.
PRINTSiPR01443. TFIID30KDSUB.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12962-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSCSGSGADP EAAPASAASA PGPAPPVSAP AALPSSTAAE NKASPAGTAG
60 70 80 90 100
GPGAGAAAGG TGPLAARAGE PAERRGAAPV SAGGAAPPEG AISNGVYVLP
110 120 130 140 150
SAANGDVKPV VSSTPLVDFL MQLEDYTPTI PDAVTGYYLN RAGFEASDPR
160 170 180 190 200
IIRLISLAAQ KFISDIANDA LQHCKMKGTA SGSSRSKSKD RKYTLTMEDL
210
TPALSEYGIN VKKPHYFT
Length:218
Mass (Da):21,711
Last modified:November 1, 1996 - v1
Checksum:i032ED5CA97EBE411
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti92 – 921I → T.1 Publication
Corresponds to variant rs3176311 [ dbSNP | Ensembl ].
VAR_013706

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U13991 mRNA. Translation: AAA62230.1.
U25816 Genomic DNA. Translation: AAB61242.1.
AF498312 Genomic DNA. Translation: AAM14627.1.
CR541943 mRNA. Translation: CAG46741.1.
CH471064 Genomic DNA. Translation: EAW68687.1.
BC012088 mRNA. Translation: AAH12088.1.
CCDSiCCDS7769.1.
PIRiA57694.
RefSeqiNP_006275.1. NM_006284.3.
UniGeneiHs.5158.

Genome annotation databases

EnsembliENST00000299424; ENSP00000299424; ENSG00000166337.
GeneIDi6881.
KEGGihsa:6881.
UCSCiuc001mej.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U13991 mRNA. Translation: AAA62230.1.
U25816 Genomic DNA. Translation: AAB61242.1.
AF498312 Genomic DNA. Translation: AAM14627.1.
CR541943 mRNA. Translation: CAG46741.1.
CH471064 Genomic DNA. Translation: EAW68687.1.
BC012088 mRNA. Translation: AAH12088.1.
CCDSiCCDS7769.1.
PIRiA57694.
RefSeqiNP_006275.1. NM_006284.3.
UniGeneiHs.5158.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2F69X-ray1.30B186-195[»]
3M53X-ray1.85B186-195[»]
3M54X-ray1.60B186-195[»]
3M55X-ray1.55B186-195[»]
3M56X-ray1.65B186-195[»]
3M57X-ray1.70B186-195[»]
3M58X-ray1.40B186-195[»]
3M59X-ray1.70B186-195[»]
3M5AX-ray1.75B186-195[»]
4J7FX-ray1.59B186-195[»]
4J7IX-ray2.56B186-195[»]
4J83X-ray1.70B186-195[»]
4J8OX-ray1.63B186-195[»]
4WV4X-ray1.91A112-212[»]
5EG2X-ray1.55B186-195[»]
ProteinModelPortaliQ12962.
SMRiQ12962. Positions 113-212.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112744. 71 interactions.
DIPiDIP-297N.
IntActiQ12962. 17 interactions.
MINTiMINT-1425043.
STRINGi9606.ENSP00000299424.

PTM databases

iPTMnetiQ12962.
PhosphoSiteiQ12962.

Polymorphism and mutation databases

BioMutaiTAF10.
DMDMi3024688.

Proteomic databases

EPDiQ12962.
MaxQBiQ12962.
PaxDbiQ12962.
PeptideAtlasiQ12962.
PRIDEiQ12962.

Protocols and materials databases

DNASUi6881.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000299424; ENSP00000299424; ENSG00000166337.
GeneIDi6881.
KEGGihsa:6881.
UCSCiuc001mej.3. human.

Organism-specific databases

CTDi6881.
GeneCardsiTAF10.
HGNCiHGNC:11543. TAF10.
HPAiCAB022405.
HPA004148.
MIMi600475. gene.
neXtProtiNX_Q12962.
PharmGKBiPA36318.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3423. Eukaryota.
COG5162. LUCA.
GeneTreeiENSGT00390000009368.
HOGENOMiHOG000285966.
HOVERGENiHBG079227.
InParanoidiQ12962.
KOiK03134.
OMAiDNTIGNP.
OrthoDBiEOG091G0UWA.
PhylomeDBiQ12962.
TreeFamiTF313156.

Enzyme and pathway databases

ReactomeiR-HSA-167161. HIV Transcription Initiation.
R-HSA-167162. RNA Polymerase II HIV Promoter Escape.
R-HSA-167172. Transcription of the HIV genome.
R-HSA-3214847. HATs acetylate histones.
R-HSA-674695. RNA Polymerase II Pre-transcription Events.
R-HSA-6804756. Regulation of TP53 Activity through Phosphorylation.
R-HSA-73776. RNA Polymerase II Promoter Escape.
R-HSA-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-HSA-75953. RNA Polymerase II Transcription Initiation.
R-HSA-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.

Miscellaneous databases

ChiTaRSiTAF10. human.
GeneWikiiTAF10.
GenomeRNAii6881.
PROiQ12962.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000166337.
CleanExiHS_TAF10.
ExpressionAtlasiQ12962. baseline and differential.
GenevisibleiQ12962. HS.

Family and domain databases

CDDicd07982. TAF10. 1 hit.
InterProiIPR003923. TFIID_30kDa.
[Graphical view]
PANTHERiPTHR21242. PTHR21242. 1 hit.
PfamiPF03540. TFIID_30kDa. 1 hit.
[Graphical view]
PIRSFiPIRSF017246. TFIID_TAF10. 1 hit.
PRINTSiPR01443. TFIID30KDSUB.
ProtoNetiSearch...

Entry informationi

Entry nameiTAF10_HUMAN
AccessioniPrimary (citable) accession number: Q12962
Secondary accession number(s): O00703, Q13175, Q6FH13
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 175 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.