Q12962 (TAF10_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription initiation factor TFIID subunit 10

Alternative name(s):
STAF28
Transcription initiation factor TFIID 30 kDa subunit
Short name=TAF(II)30
Short name=TAFII-30
Short name=TAFII30

Gene names

Name:	TAF10
Synonyms:	TAF2A, TAF2H, TAFII30

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	218 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	TAFs are components of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TIIFD is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors.
Subunit structure	TFIID and PCAF are composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). TBP is not part of TFTC. Component of the PCAF complex, at least composed of TADA2L/ADA2, TADA3L/ADA3, SUPT3H, TAF5L TAF6L, TAF9, TAF10, TAF12 and TRRAP. Component of the TFTC-HAT complex, at least composed of TAF5L, TAF6L, TADA3L, SUPT3H, TAF2, TAF4, TAF5, GCN5L2/GCN5, TAF10 and TRRAP. Component of the STAGA transcription coactivator-HAT complex, at least composed of SUPT3H, GCN5L2, TAF5L, TAF6L, SUPT7L, TADA3L, TAD1L, TAF10, TAF12, TRRAP and TAF9. The STAGA core complex is associated with a subcomplex required for histone deubiquitinylation composed of ATXN7L3, ENY2 and USP22. Interacts with TAF3. Ref.7 Ref.11
Subcellular location	Nucleus Ref.7 Ref.8.
Domain	The [KR]-[STA]-K motif is specifically recognized by the SETD7 methyltransferase.
Post-translational modification	Monomethylated at Lys-189 by SETD7, leading to increase its affinity for RNA polymerase II. Ref.12 Ref.13
Sequence similarities	Belongs to the TAF10 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
PTM	Methylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	histone H3 acetylation Inferred from direct assay Ref.8Ref.7. Source: UniProtKB histone deubiquitination Inferred from direct assay Ref.15. Source: UniProtKB protein homooligomerization Inferred from direct assay Ref.1. Source: UniProtKB regulation of transcription, DNA-dependent Inferred from direct assay. Source: GOC transcription elongation from RNA polymerase II promoter Traceable author statement. Source: Reactome transcription initiation from RNA polymerase II promoter Inferred from direct assay. Source: UniProtKB viral reproduction Traceable author statement. Source: Reactome
Cellular component	PCAF complex Inferred from direct assay Ref.7. Source: UniProtKB STAGA complex Inferred from direct assay Ref.8. Source: UniProtKB perinuclear region of cytoplasm Inferred from direct assay. Source: HGNC transcription factor TFIID complex Inferred from direct assay Ref.12Ref.1. Source: UniProtKB transcription factor TFTC complex Inferred from direct assay Ref.10. Source: UniProtKB
Molecular function	RNA polymerase binding Inferred from direct assay Ref.12. Source: UniProtKB estrogen receptor binding Inferred from direct assay Ref.1. Source: UniProtKB transcription coactivator activity Inferred from direct assay Ref.8. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ATXN7	O15265	6	EBI-708376,EBI-708350
SETD7	Q8WTS6	2	EBI-708376,EBI-1268586

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 218	218	Transcription initiation factor TFIID subunit 10		PRO_0000118897
Regions
	Motif	187 – 189	3	[KR]-[STA]-K motif
Amino acid modifications
	Modified residue	189	1	N6-methyllysine Ref.12 Ref.13
Natural variations
	Natural variant	92	1	I → T. Ref.3 Corresponds to variant rs3176311 [ dbSNP | Ensembl ].		VAR_013706
Experimental info
	Mutagenesis	189	1	K → Q: Abolishes methylation. Ref.12

Sequences

	Sequence		Length	Mass (Da)
	Q12962 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 032ED5CA97EBE411	FASTA	218	21,711
	10 20 30 40 50 60 MSCSGSGADP EAAPASAASA PGPAPPVSAP AALPSSTAAE NKASPAGTAG GPGAGAAAGG 70 80 90 100 110 120 TGPLAARAGE PAERRGAAPV SAGGAAPPEG AISNGVYVLP SAANGDVKPV VSSTPLVDFL 130 140 150 160 170 180 MQLEDYTPTI PDAVTGYYLN RAGFEASDPR IIRLISLAAQ KFISDIANDA LQHCKMKGTA 190 200 210 SGSSRSKSKD RKYTLTMEDL TPALSEYGIN VKKPHYFT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human TAFII30 is present in a distinct TFIID complex and is required for transcriptional activation by the estrogen receptor." Jacq X., Brou C., Lutz Y., Davidson I., Chambon P., Tora L. Cell 79:107-117(1994) [PubMed: 7923369] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"Organization and chromosomal localization of the gene (TAF2H) encoding the human TBP-associated factor II 30 (TAFII30)." Scheer E., Mattei M.-G., Jacq X., Chambon P., Tora L. Genomics 29:269-272(1995) [PubMed: 8530084] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Placenta.
[3]	NIEHS SNPs program Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT THR-92.
[4]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[7]	"Histone-like TAFs within the PCAF histone acetylase complex." Ogryzko V.V., Kotani T., Zhang X., Schiltz R.L., Howard T., Yang X.-J., Howard B.H., Qin J., Nakatani Y. Cell 94:35-44(1998) [PubMed: 9674425] [Abstract] Cited for: PROTEIN SEQUENCE OF 43-67, SUBUNIT, SUBCELLULAR LOCATION.
[8]	"Human STAGA complex is a chromatin-acetylating transcription coactivator that interacts with pre-mRNA splicing and DNA damage-binding factors in vivo." Martinez E., Palhan V.B., Tjernberg A., Lymar E.S., Gamper A.M., Kundu T.K., Chait B.T., Roeder R.G. Mol. Cell. Biol. 21:6782-6795(2001) [PubMed: 11564863] [Abstract] Cited for: MASS SPECTROMETRY, SUBCELLULAR LOCATION, IDENTIFICATION IN THE STAGA COMPLEX WITH SUPT3H; GCN5L2; KIAA0764; TAF5L; TAF6L; TRRAP; TADA3L; TAF12 AND TAF9.
[9]	"The 400 kDa subunit of the PCAF histone acetylase complex belongs to the ATM superfamily." Vassilev A., Yamauchi J., Kotani T., Prives C., Avantaggiati M.L., Qin J., Nakatani Y. Mol. Cell 2:869-875(1998) [PubMed: 9885574] [Abstract] Cited for: IDENTIFICATION IN THE PCAF COMPLEX WITH TADA2L; TADA3L; TAF5L; SUPT3H; TAF6L; TAF12; TRRAP AND TAF9.
[10]	"Identification of TATA-binding protein-free TAFII-containing complex subunits suggests a role in nucleosome acetylation and signal transduction." Brand M., Yamamoto K., Staub A., Tora L. J. Biol. Chem. 274:18285-18289(1999) [PubMed: 10373431] [Abstract] Cited for: IDENTIFICATION IN THE TFTC-HAT COMPLEX WITH TAF5L; TAF6L; TADA3L; SUPT3H; TAF2; TAF4; TAF5; TRRAP; GCN5L2 AND TAF10.
[11]	"The TFIID components human TAFII140 and Drosophila BIP2 (TAFII155) are novel metazoan homologues of yeast TAFII47 containing a histone fold and a PHD finger." Gangloff Y.G., Pointud J.-C., Thuault S., Carre L., Romier C., Muratoglu S., Brand M., Tora L., Couderc J.-L., Davidson I. Mol. Cell. Biol. 21:5109-5121(2001) [PubMed: 11438666] [Abstract] Cited for: INTERACTION WITH TAF3.
[12]	"Gene-specific modulation of TAF10 function by SET9-mediated methylation." Kouskouti A., Scheer E., Staub A., Tora L., Talianidis I. Mol. Cell 14:175-182(2004) [PubMed: 15099517] [Abstract] Cited for: METHYLATION AT LYS-189, MUTAGENESIS OF LYS-189.
[13]	"Structural basis for the methylation site specificity of SET7/9." Couture J.-F., Collazo E., Hauk G., Trievel R.C. Nat. Struct. Mol. Biol. 13:140-146(2006) [PubMed: 16415881] [Abstract] Cited for: MOTIF, METHYLATION AT LYS-189.
[14]	"Novel subunits of the TATA binding protein free TAFII-containing transcription complex identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry following one-dimensional gel electrophoresis." Cavusoglu N., Brand M., Tora L., van Dorsselaer A. Proteomics 3:217-223(2003) [PubMed: 12601814] [Abstract] Cited for: IDENTIFICATION IN THE TFTC-HAT COMPLEX, MASS SPECTROMETRY.
[15]	"A TFTC/STAGA module mediates histone H2A and H2B deubiquitination, coactivates nuclear receptors, and counteracts heterochromatin silencing." Zhao Y., Lang G., Ito S., Bonnet J., Metzger E., Sawatsubashi S., Suzuki E., Le Guezennec X., Stunnenberg H.G., Krasnov A., Georgieva S.G., Schuele R., Takeyama K., Kato S., Tora L., Devys D. Mol. Cell 29:92-101(2008) [PubMed: 18206972] [Abstract] Cited for: IDENTIFICATION IN STAGA COMPLEX.
[16]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Web resources

NIEHS-SNPs

Cross-references

Sequence databases
EMBL GenBank DDBJ	U13991 mRNA. Translation: AAA62230.1. U25816 Genomic DNA. Translation: AAB61242.1. AF498312 Genomic DNA. Translation: AAM14627.1. CR541943 mRNA. Translation: CAG46741.1. CH471064 Genomic DNA. Translation: EAW68687.1. BC012088 mRNA. Translation: AAH12088.1.
IPI	IPI00030364.
PIR	A57694.
RefSeq	NP_006275.1. NM_006284.3.
UniGene	Hs.5158.
3D structure databases
ProteinModelPortal	Q12962.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-29046N. DIP-297N.
IntAct	Q12962. 14 interactions.
MINT	MINT-1425043.
STRING	Q12962.
PTM databases
PhosphoSite	Q12962.
Polymorphism databases
DMDM	3024688.
Proteomic databases
PeptideAtlas	Q12962.
PRIDE	Q12962.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000299424; ENSP00000299424; ENSG00000166337.
GeneID	6881.
KEGG	hsa:6881.
UCSC	uc001mej.1. human.
Organism-specific databases
CTD	6881.
GeneCards	GC11M006627.
H-InvDB	HIX0201651.
HGNC	HGNC:11543. TAF10.
HPA	CAB022405. HPA004148.
MIM	600475. gene.
neXtProt	NX_Q12962.
PharmGKB	PA36318.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG20073.
GeneTree	ENSGT00390000009368.
HOGENOM	HBG398309.
HOVERGEN	HBG079227.
InParanoid	Q12962.
OMA	PPEGAIS.
OrthoDB	EOG4DFPPR.
PhylomeDB	Q12962.
Enzyme and pathway databases
Reactome	REACT_1788. Transcription. REACT_6185. HIV Infection. REACT_71. Gene Expression.
Gene expression databases
ArrayExpress	Q12962.
Bgee	Q12962.
CleanEx	HS_TAF10.
Genevestigator	Q12962.
GermOnline	ENSG00000166337. Homo sapiens.
Family and domain databases
InterPro	IPR003923. TFIID_30kDa. [Graphical view]
KO	K03134.
PANTHER	PTHR21242. TFIID_30kD. 1 hit.
Pfam	PF03540. TFIID_30kDa. 1 hit. [Graphical view]
PIRSF	PIRSF017246. TFIID_TAF10. 1 hit.
PRINTS	PR01443. TFIID30KDSUB.
ProtoNet	Search...
Other
NextBio	26883.
SOURCE	Search...

Entry information

Entry name

TAF10_HUMAN

Accession

Primary (citable) accession number: Q12962
Secondary accession number(s): O00703, Q13175, Q6FH13

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 128 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.