Reviewed, UniProtKB/Swiss-Prot Q12947 (FOXF2_HUMAN)

Names and origin

Protein names

Recommended name:
    Forkhead box protein F2
Alternative name(s):
    Forkhead-related transcription factor 2
    Forkhead-related activator 2
      Short name=FREAC-2
    Forkhead-related protein FKHL6

Gene names

Name:	FOXF2
Synonyms:	FKHL6, FREAC2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	444 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Probable transcription activator for a number of lung-specific genes.
Subunit structure	Interacts with the transcription factors TBP and TFIIB. Ref.4
Subcellular location	Nucleus.
Tissue specificity	Lung and placenta.
Domain	Two activation domains, AD1 and AD2, C-terminal of (and distinct from) the forkhead domains are necessary for transcriptional activation. Ref.4
Sequence similarities	Contains 1 fork-head DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Activator
Gene Ontology (GO)
Biological process	positive regulation of transcription, DNA-dependent Ref.2 Inferred from direct assay. Source: UniProtKB transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	transcription factor complex Ref.4 Traceable author statement. Source: ProtInc
Molecular function	protein binding Inferred from physical interaction. Source: IntAct sequence-specific DNA binding Ref.2 Ref.3 Inferred from direct assay. Source: UniProtKB transcription activator activity Ref.2 Inferred from direct assay. Source: UniProtKB transcription factor activity Ref.4 Non-traceable author statement. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ABL1	P00519	1	EBI-1752316,EBI-375543
FYN	P06241	1	EBI-1752316,EBI-515315

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 444	444	Forkhead box protein F2		PRO_0000091834
Regions
	DNA binding	99 – 190	92	Fork-head
	Compositional bias	33 – 41	9	Poly-Ala
	Compositional bias	46 – 55	10	Poly-Ser
	Compositional bias	58 – 67	10	Poly-Ser
	Compositional bias	77 – 84	8	Poly-Gly
	Compositional bias	262 – 272	11	Poly-His
	Compositional bias	301 – 306	6	Poly-Gly
	Compositional bias	312 – 315	4	Poly-Ser
	Compositional bias	429 – 432	4	Poly-His

Sequences

	Sequence		Length	Mass (Da)
	Q12947-1 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: 32BDC5F373CFB147	FASTA	444	45,993
	10 20 30 40 50 60 MTTEGGPPPA PLRRACSPVP GALQAALMSP PPAAAAAAAA APETTSSSSS SSSASCASSS 70 80 90 100 110 120 SSSNSASAPS AACKSAGGGG AGAGSGGAKK ASSGLRRPEK PPYSYIALIV MAIQSSPSKR 130 140 150 160 170 180 LTLSEIYQFL QARFPFFRGA YQGWKNSVRH NLSLNECFIK LPKGLGRPGK GHYWTIDPAS 190 200 210 220 230 240 EFMFEEGSFR RRPRGFRRKC QALKPMYHRV VSGLGFGASL LPQGFDFQAP PSAPLGCHSQ 250 260 270 280 290 300 GGYGGLDMMP AGYDAGAGAP SHAHPHHHHH HHVPHMSPNP GSTYMASCPV PAGPGGVGAA 310 320 330 340 350 360 GGGGGGDYGP DSSSSPVPSS PAMASAIECH SPYTSPAAHW SSPGASPYLK QPPALTPSSN 370 380 390 400 410 420 PAASAGLHSS MSSYSLEQSY LHQNAREDLS VGLPRYQHHS TPVCDRKDFV LNFNGISSFH 430 440 PSASGSYYHH HHQSVCQDIK PCVM

References

[1]	"The two-exon gene of the human forkhead transcription factor FREAC-2 (FKHL6) is located at 6p25.3." Blixt A., Mahlapuu M., Bjursell C., Darnfors C., Johannesson T., Enerbaeck S., Carlsson P. Genomics 53:387-390(1998) [PubMed: 9799607] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Differential activation of lung-specific genes by two forkhead proteins, FREAC-1 and FREAC-2." Hellqvist M., Mahlapuu M., Samuelsson L., Enerbaeck S., Carlsson P. J. Biol. Chem. 271:4482-4490(1996) [PubMed: 8626802] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 37-444. Tissue: Lung.
[3]	"Cloning and characterization of seven human forkhead proteins: binding site specificity and DNA bending." Pierrou S., Hellqvist M., Samuelsson L., Enerbaeck S., Carlsson P. EMBO J. 13:5002-5012(1994) [PubMed: 7957066] [Abstract] Cited for: PARTIAL NUCLEOTIDE SEQUENCE [MRNA].
[4]	"The human forkhead protein FREAC-2 contains two functionally redundant activation domains and interacts with TBP and TFIIB." Hellqvist M., Mahlapuu M., Blixt A., Enerbaeck S., Carlsson P. J. Biol. Chem. 273:23335-23343(1998) [PubMed: 9722567] [Abstract] Cited for: DOMAIN, INTERACTION WITH TBP AND TFIIB.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF084939, AF084938 Genomic DNA. Translation: AAD19875.1. U13220 mRNA. Translation: AAC32226.1.
IPI	IPI00030319.
PIR	S51625. T09474.
RefSeq	NP_001443.1.
UniGene	Hs.484423
3D structure databases
HSSP	HSSP built from PDB template 1D5V based on UniProtKB Q99958.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q12947. 2 interactions.
Proteomic databases
PRIDE	Q12947.
Genome annotation databases
Ensembl	ENSG00000137273. Homo sapiens. [Contig view]
GeneID	2295.
KEGG	hsa:2295.
Organism-specific databases
GeneCards	GC06P001335.
H-InvDB	HIX0032954.
HGNC	HGNC:3810. FOXF2.
MIM	603250. gene.
PharmGKB	PA28227.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q12947.
HOVERGEN	Q12947.
OMA	Q12947. PSHAHPH.
Gene expression databases
ArrayExpress	Q12947.
Bgee	Q12947.
CleanEx	HS_FOXF2.
GermOnline	ENSG00000137273. Homo sapiens.
Family and domain databases
InterPro	IPR001766. TF_fork_head. IPR018122. TF_fork_head_CS. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
PANTHER	PTHR11829. Fork_box_protein. 1 hit.
Pfam	PF00250. Fork_head. 1 hit. [Graphical view]
PRINTS	PR00053. FORKHEAD.
ProDom	PD000425. TF_Fork_head. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00339. FH. 1 hit. [Graphical view]
PROSITE	PS00657. FORK_HEAD_1. 1 hit. PS00658. FORK_HEAD_2. 1 hit. PS50039. FORK_HEAD_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	9315.
SOURCE	Search...

Entry information

Entry name

FOXF2_HUMAN

Accession

Primary (citable) accession number: Q12947
Secondary accession number(s): Q9UQ85

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 30, 2000
Last modified:	June 16, 2009
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)